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Antibiotic resistance pattern and virulence genes content in avian pathogenic Escherichia coli (APEC) from broiler chickens in Chitwan, Nepal.
BMC Vet Res. 2018 Mar 27; 14(1):113.BV

Abstract

BACKGROUND

Avian pathogenic Escherichia coli (APEC) are causative agent of extraintestinal infections, collectively known as colibacillosis, which results significant losses in poultry industries. The extraintestinal survival of E. coli is facilitated by numerous virulence factors which are coded by virulence genes. This study was conducted to find out the pattern of antibiotic resistance and virulence genes content in the APEC strains isolated from broiler chickens at National Avian Disease Investigation Laboratory and Veterinary Teaching Hospital, Rampur, Chitwan, Nepal.

RESULTS

A total of 50 E. coli strains were isolated from 50 colibacillosis suspected broiler chickens. Out of 50 isolates of E. coli, 47 (94%) showed resistant to three or more antimicrobials. The highest levels (22%) of multidrug-resistant E. coli were observed for five different types of antimicrobials. Antibiogram profiles of 50 E. coli strains showed the maximum resistance to ampicillin (98%), followed by co-trimoxazole (90%), and doxycycline (62%). The highest intermediate resistance was shown by colistin (50%) and the highest sensitivity was against amikacin (84%), followed by nitrofurantoin (55%). Based on the genetic criteria, 45 (90%) E. coli isolates were considered as pathogenic (APEC) which contained more than five virulence genes. Out of total APEC genes detected, we found the combination of iss, iucD, hlyF, ompT, iroN, and iutA genes were mostly associated with the APEC and additionally, to some lesser extent irp2, papC, Cva/cvi, and tsh genes showed the critical role for virulent traits of APEC strains.

CONCLUSION

In this study, high prevalent of antimicrobial resistant pattern was found with avian pathogenic E. coli strains isolated from broiler chickens. To our knowledge, this is the first molecular analysis which confirmed the prevalence of APEC strains in poultry sector in Nepal. These finding suggest the need of surveillance and intervention system to control misuse of antibiotics and APEC outbreak in the poultry farm.

Authors+Show Affiliations

Department of Drug Administration, Government of Nepal, Bijulibazar, Kathmandu, Nepal.Center for Biotechnology, Agriculture and Forestry University, Rampur, Chitwan, Nepal.Center for Biotechnology, Agriculture and Forestry University, Rampur, Chitwan, Nepal.Department of Veterinary Microbiology and Parasitology, Agriculture and Forestry University, Rampur, Chitwan, Nepal.Department of Veterinary Science & Animal Husbandry, Himalayan College of Agriculture Sciences and Technology, Kathmandu, Nepal.Department of Microbiology, National College, Kathmandu, Nepal.Department of Microbiology, Balkumari College, Bharatpur, Chitwan, Nepal.Department of Microbiology, Prithu Technical College, Institute of Agriculture and Animal Science, Tribhuvan University, Lamahi, Dang, Nepal. dhirajchaudhary2042@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29587768

Citation

Subedi, Manita, et al. "Antibiotic Resistance Pattern and Virulence Genes Content in Avian Pathogenic Escherichia Coli (APEC) From Broiler Chickens in Chitwan, Nepal." BMC Veterinary Research, vol. 14, no. 1, 2018, p. 113.
Subedi M, Luitel H, Devkota B, et al. Antibiotic resistance pattern and virulence genes content in avian pathogenic Escherichia coli (APEC) from broiler chickens in Chitwan, Nepal. BMC Vet Res. 2018;14(1):113.
Subedi, M., Luitel, H., Devkota, B., Bhattarai, R. K., Phuyal, S., Panthi, P., Shrestha, A., & Chaudhary, D. K. (2018). Antibiotic resistance pattern and virulence genes content in avian pathogenic Escherichia coli (APEC) from broiler chickens in Chitwan, Nepal. BMC Veterinary Research, 14(1), 113. https://doi.org/10.1186/s12917-018-1442-z
Subedi M, et al. Antibiotic Resistance Pattern and Virulence Genes Content in Avian Pathogenic Escherichia Coli (APEC) From Broiler Chickens in Chitwan, Nepal. BMC Vet Res. 2018 Mar 27;14(1):113. PubMed PMID: 29587768.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibiotic resistance pattern and virulence genes content in avian pathogenic Escherichia coli (APEC) from broiler chickens in Chitwan, Nepal. AU - Subedi,Manita, AU - Luitel,Himal, AU - Devkota,Bhuminanda, AU - Bhattarai,Rebanta Kumar, AU - Phuyal,Sarita, AU - Panthi,Prabhat, AU - Shrestha,Anil, AU - Chaudhary,Dhiraj Kumar, Y1 - 2018/03/27/ PY - 2017/11/01/received PY - 2018/03/22/accepted PY - 2018/3/29/entrez PY - 2018/3/29/pubmed PY - 2018/8/31/medline KW - Antimicrobial resistance KW - Avian pathogenic Escherichia coli (APEC) KW - Broiler chicken KW - PCR KW - Virulence gene SP - 113 EP - 113 JF - BMC veterinary research JO - BMC Vet. Res. VL - 14 IS - 1 N2 - BACKGROUND: Avian pathogenic Escherichia coli (APEC) are causative agent of extraintestinal infections, collectively known as colibacillosis, which results significant losses in poultry industries. The extraintestinal survival of E. coli is facilitated by numerous virulence factors which are coded by virulence genes. This study was conducted to find out the pattern of antibiotic resistance and virulence genes content in the APEC strains isolated from broiler chickens at National Avian Disease Investigation Laboratory and Veterinary Teaching Hospital, Rampur, Chitwan, Nepal. RESULTS: A total of 50 E. coli strains were isolated from 50 colibacillosis suspected broiler chickens. Out of 50 isolates of E. coli, 47 (94%) showed resistant to three or more antimicrobials. The highest levels (22%) of multidrug-resistant E. coli were observed for five different types of antimicrobials. Antibiogram profiles of 50 E. coli strains showed the maximum resistance to ampicillin (98%), followed by co-trimoxazole (90%), and doxycycline (62%). The highest intermediate resistance was shown by colistin (50%) and the highest sensitivity was against amikacin (84%), followed by nitrofurantoin (55%). Based on the genetic criteria, 45 (90%) E. coli isolates were considered as pathogenic (APEC) which contained more than five virulence genes. Out of total APEC genes detected, we found the combination of iss, iucD, hlyF, ompT, iroN, and iutA genes were mostly associated with the APEC and additionally, to some lesser extent irp2, papC, Cva/cvi, and tsh genes showed the critical role for virulent traits of APEC strains. CONCLUSION: In this study, high prevalent of antimicrobial resistant pattern was found with avian pathogenic E. coli strains isolated from broiler chickens. To our knowledge, this is the first molecular analysis which confirmed the prevalence of APEC strains in poultry sector in Nepal. These finding suggest the need of surveillance and intervention system to control misuse of antibiotics and APEC outbreak in the poultry farm. SN - 1746-6148 UR - https://www.unboundmedicine.com/medline/citation/29587768/Antibiotic_resistance_pattern_and_virulence_genes_content_in_avian_pathogenic_Escherichia_coli__APEC__from_broiler_chickens_in_Chitwan_Nepal_ L2 - https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-018-1442-z DB - PRIME DP - Unbound Medicine ER -