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Impedimetric detection of bacteria by using a microfluidic chip and silver nanoparticle based signal enhancement.
Mikrochim Acta. 2018 02 19; 185(3):184.MA

Abstract

The authors describe a method that can significantly improve the performance of impedimetric detection of bacteria. A multifunctional microfluidic chip was designed consisting of interdigitated microelectrodes and a micro-mixing zone with a Tesla structure. This maximizes the coating of bacterial surfaces with nanoparticles and results in improved impedimetric detection. The method was applied to the detection of Escherichia coli O157:H7 (E. coli). Silver enhancement was accomplished by coating E.coli with the cationic polymer diallyldimethylammonium chloride (PDDA) to form positively charged E. coli/PDDA complexes. Then, gold nanoparticles (AuNPs) were added, and the resulting E. coli/PDDA/AuNPs complexes were collected at interdigitated electrodes via positive dielectrophoresis (pDEP). A silver adduct was then formed on the E. coli/PDDA/AuNP complexes by using silver enhancement solutions and by using the AuNPs as catalysts. The combination of pDEP based capture and of using silver adducts reduces impedance by increasing the conductivity of the solution and the double layer capacitance around the microelectrodes. Impedance decreases linearly in the 2 × 103-2 × 105 cfu·mL-1 E. coli concentration range, with a 500 cfu·mL-1 detection limit. Egg shell wash samples and tap water spiked with E. coli were successfully used for validation, and this demonstrates the practical application of this method. Graphical abstract Schematic representation of the AuNP@Ag enhancement method integrated with multifunctional microfluidic chip platform for impedimetric quantitation of bacteria. The method significantly improves the performance of impedimetric detection of bacteria.

Authors+Show Affiliations

College of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044, China. Key Principle Laboratory of Fundamental Science of Micro/Nano-Device and System Technology, Chongqing, 400044, China. National Center for International Research of Micro/Nano-System and New Material Technology, Chongqing, 400044, China.College of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044, China. xuyibbd@sina.com. Key Principle Laboratory of Fundamental Science of Micro/Nano-Device and System Technology, Chongqing, 400044, China. xuyibbd@sina.com. National Center for International Research of Micro/Nano-System and New Material Technology, Chongqing, 400044, China. xuyibbd@sina.com.Department of Agricultural and Biological Engineering, Bindley Bioscience Center, Purdue University, West Lafayette, IN, 47907, USA.Department of Agricultural and Biological Engineering, Bindley Bioscience Center, Purdue University, West Lafayette, IN, 47907, USA.Department of Agricultural and Biological Engineering, Bindley Bioscience Center, Purdue University, West Lafayette, IN, 47907, USA.College of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044, China. Key Principle Laboratory of Fundamental Science of Micro/Nano-Device and System Technology, Chongqing, 400044, China. National Center for International Research of Micro/Nano-System and New Material Technology, Chongqing, 400044, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29594583

Citation

Wang, Renjie, et al. "Impedimetric Detection of Bacteria By Using a Microfluidic Chip and Silver Nanoparticle Based Signal Enhancement." Mikrochimica Acta, vol. 185, no. 3, 2018, p. 184.
Wang R, Xu Y, Sors T, et al. Impedimetric detection of bacteria by using a microfluidic chip and silver nanoparticle based signal enhancement. Mikrochim Acta. 2018;185(3):184.
Wang, R., Xu, Y., Sors, T., Irudayaraj, J., Ren, W., & Wang, R. (2018). Impedimetric detection of bacteria by using a microfluidic chip and silver nanoparticle based signal enhancement. Mikrochimica Acta, 185(3), 184. https://doi.org/10.1007/s00604-017-2645-x
Wang R, et al. Impedimetric Detection of Bacteria By Using a Microfluidic Chip and Silver Nanoparticle Based Signal Enhancement. Mikrochim Acta. 2018 02 19;185(3):184. PubMed PMID: 29594583.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Impedimetric detection of bacteria by using a microfluidic chip and silver nanoparticle based signal enhancement. AU - Wang,Renjie, AU - Xu,Yi, AU - Sors,Thomas, AU - Irudayaraj,Joseph, AU - Ren,Wen, AU - Wang,Rong, Y1 - 2018/02/19/ PY - 2017/09/10/received PY - 2017/12/28/accepted PY - 2018/3/30/entrez PY - 2018/3/30/pubmed PY - 2019/1/31/medline KW - Bacterial detection KW - Electrochemical impedance spectroscopy KW - Escherichia coli O157:H7 KW - Gold nanoparticles KW - Interdigitated microelectrodes KW - Positive dielectrophoresis KW - Silver enhancement KW - Tesla structure SP - 184 EP - 184 JF - Mikrochimica acta JO - Mikrochim Acta VL - 185 IS - 3 N2 - The authors describe a method that can significantly improve the performance of impedimetric detection of bacteria. A multifunctional microfluidic chip was designed consisting of interdigitated microelectrodes and a micro-mixing zone with a Tesla structure. This maximizes the coating of bacterial surfaces with nanoparticles and results in improved impedimetric detection. The method was applied to the detection of Escherichia coli O157:H7 (E. coli). Silver enhancement was accomplished by coating E.coli with the cationic polymer diallyldimethylammonium chloride (PDDA) to form positively charged E. coli/PDDA complexes. Then, gold nanoparticles (AuNPs) were added, and the resulting E. coli/PDDA/AuNPs complexes were collected at interdigitated electrodes via positive dielectrophoresis (pDEP). A silver adduct was then formed on the E. coli/PDDA/AuNP complexes by using silver enhancement solutions and by using the AuNPs as catalysts. The combination of pDEP based capture and of using silver adducts reduces impedance by increasing the conductivity of the solution and the double layer capacitance around the microelectrodes. Impedance decreases linearly in the 2 × 103-2 × 105 cfu·mL-1 E. coli concentration range, with a 500 cfu·mL-1 detection limit. Egg shell wash samples and tap water spiked with E. coli were successfully used for validation, and this demonstrates the practical application of this method. Graphical abstract Schematic representation of the AuNP@Ag enhancement method integrated with multifunctional microfluidic chip platform for impedimetric quantitation of bacteria. The method significantly improves the performance of impedimetric detection of bacteria. SN - 1436-5073 UR - https://www.unboundmedicine.com/medline/citation/29594583/Impedimetric_detection_of_bacteria_by_using_a_microfluidic_chip_and_silver_nanoparticle_based_signal_enhancement_ L2 - https://scite.ai/reports/29594583 DB - PRIME DP - Unbound Medicine ER -