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An aptamer-based colorimetric lead(II) assay based on the use of gold nanoparticles modified with dsDNA and exonuclease I.
Mikrochim Acta. 2018 02 01; 185(2):151.MA

Abstract

The authors describe a colorimetric method for the sensitive and selective detection of Pb(II). It is based on the use exonuclease I (Exo I), a Pb(II)-binding aptamer bound to gold nanoparticles (AuNPs), and a DNA strand that complementary to the aptamer. In the absence of Pb(II), the dsDNA on the AuNPs prevents aggregation of the AuNPs in the presence of NaCl. In the presence of Pb(II), however, the aptamer binds Pb(II) and complementary strand is released and digested by Exo I. As a result, the solution of AuNPs undergoes a color change from red to purple if salt is added to the sample. The assay is selective for Pb(II) and has a limit of detection as low as 2.4 nM. It was successfully applied to the determination of Pb(II) in spiked tap water. Graphical abstract Schematic presentation of the aptamer based method for Pb2+ detection via salt-induced aggregation of gold nanoparticles and colorimetric quantitation.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Shahdordizadeh M
Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, 91778-99191, Iran.
Yazdian-Robati R
Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, 91778-99191, Iran.
Ansari N
Department of Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Science, Mashhad, 91778-99191, Iran.
Ramezani M
Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, 91778-99191, Iran.
Abnous K
Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, 91778-99191, Iran. abnouskh@mums.ac.ir.
Taghdisi SM
Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, 91778-99191, Iran. taghdisihm@mums.ac.ir.

MeSH

Aptamers, NucleotideBiosensing TechniquesColorimetryDNADNA Repair EnzymesExodeoxyribonucleasesGoldHumansLeadMetal NanoparticlesWater Pollutants, Chemical

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29594698

Citation

Shahdordizadeh, Mahin, et al. "An Aptamer-based Colorimetric lead(II) Assay Based On the Use of Gold Nanoparticles Modified With dsDNA and Exonuclease I." Mikrochimica Acta, vol. 185, no. 2, 2018, p. 151.
Shahdordizadeh M, Yazdian-Robati R, Ansari N, et al. An aptamer-based colorimetric lead(II) assay based on the use of gold nanoparticles modified with dsDNA and exonuclease I. Mikrochim Acta. 2018;185(2):151.
Shahdordizadeh, M., Yazdian-Robati, R., Ansari, N., Ramezani, M., Abnous, K., & Taghdisi, S. M. (2018). An aptamer-based colorimetric lead(II) assay based on the use of gold nanoparticles modified with dsDNA and exonuclease I. Mikrochimica Acta, 185(2), 151. https://doi.org/10.1007/s00604-018-2699-4
Shahdordizadeh M, et al. An Aptamer-based Colorimetric lead(II) Assay Based On the Use of Gold Nanoparticles Modified With dsDNA and Exonuclease I. Mikrochim Acta. 2018 02 1;185(2):151. PubMed PMID: 29594698.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An aptamer-based colorimetric lead(II) assay based on the use of gold nanoparticles modified with dsDNA and exonuclease I. AU - Shahdordizadeh,Mahin, AU - Yazdian-Robati,Rezvan, AU - Ansari,Najmeh, AU - Ramezani,Mohammad, AU - Abnous,Khalil, AU - Taghdisi,Seyed Mohammad, Y1 - 2018/02/01/ PY - 2017/11/03/received PY - 2018/01/20/accepted PY - 2018/3/30/entrez PY - 2018/3/30/pubmed PY - 2019/2/12/medline KW - Color change KW - Enzyme KW - Salt-induced aggregation KW - Tap water SP - 151 EP - 151 JF - Mikrochimica acta JO - Mikrochim Acta VL - 185 IS - 2 N2 - The authors describe a colorimetric method for the sensitive and selective detection of Pb(II). It is based on the use exonuclease I (Exo I), a Pb(II)-binding aptamer bound to gold nanoparticles (AuNPs), and a DNA strand that complementary to the aptamer. In the absence of Pb(II), the dsDNA on the AuNPs prevents aggregation of the AuNPs in the presence of NaCl. In the presence of Pb(II), however, the aptamer binds Pb(II) and complementary strand is released and digested by Exo I. As a result, the solution of AuNPs undergoes a color change from red to purple if salt is added to the sample. The assay is selective for Pb(II) and has a limit of detection as low as 2.4 nM. It was successfully applied to the determination of Pb(II) in spiked tap water. Graphical abstract Schematic presentation of the aptamer based method for Pb2+ detection via salt-induced aggregation of gold nanoparticles and colorimetric quantitation. SN - 1436-5073 UR - https://www.unboundmedicine.com/medline/citation/29594698/An_aptamer_based_colorimetric_lead_II__assay_based_on_the_use_of_gold_nanoparticles_modified_with_dsDNA_and_exonuclease_I_ DB - PRIME DP - Unbound Medicine ER -