Tags

Type your tag names separated by a space and hit enter

Investigating the possible mechanisms involved in adenosine preconditioning-induced cardioprotection in rats.
Cardiovasc Ther. 2018 Jun; 36(3):e12328.CT

Abstract

BACKGROUND

Adenosine is a breakdown product of adenosine triphosphate and plays an important role in pharmacological preconditioning. The cardioprotective effects of adenosine preconditioning are well established. However, the possible mechanisms need to be explored.

AIM

This study was aimed to investigate the possible mechanisms involved in adenosine preconditioning-induced cardioprotection in rats.

METHODS

Rat heart was isolated and perfused on Langendorff apparatus. Global ischemia for 30 minutes followed by reperfusion for 120 minutes was employed to produce myocardial injury. Myocardial injury was assessed by measuring myocardial infarct size, release of lactate dehydrogenase (LDH) and creatine kinase (CK) in the coronary effluent and hemodynamic parameters including left ventricular developed pressure (LVDP), dp/dtmax, and dp/dtmin . Serum nitrite levels were measured as an index of nitric oxide release in blood.

RESULTS

Adenosine (4 mg/kg) preconditioning significantly decreased ischemia-reperfusion-induced increase in LDH, CK release, infarct size, improved LVDP, dp/dtmax and dp/dtmin, and increased serum nitrite levels. Pretreatment with L-NAME, a specific NOS inhibitor, (5 mg/kg) and montelukast, leukotriene receptor antagonist, (10 mg/kg) significantly abrogated the cardioprotective effect of adenosine preconditioning. However, seratrodast, thromboxane A2 antagonist, (15 mg/kg) had no effect on adenosine-induced cardioprotection. Sodium nitroprusside (SNP) preconditioning also produced cardioprotective effects. However, caffeine (20 mg/kg) (adenosine receptor blocker) and seratrodast (15 mg/kg) had no effect on SNP-induced cardioprotection. Administration of montelukast abrogated the cardioprotective effects of SNP preconditioning-induced cardioprotection.

CONCLUSION

Adenosine preconditioning may increase the release of nitric oxide, which in turn may increase the release of cysteinyl leukotrienes to confer cardioprotection.

Authors+Show Affiliations

Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala, India.Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala, India.Laboratory of Experimental Cardiology, Federal State Budgetary Scientific Institution, Research Institute for Cardiology, Tomsk, Russia.Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala, India.Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29604187

Citation

Singh, Lovedeep, et al. "Investigating the Possible Mechanisms Involved in Adenosine Preconditioning-induced Cardioprotection in Rats." Cardiovascular Therapeutics, vol. 36, no. 3, 2018, pp. e12328.
Singh L, Virdi JK, Maslov LN, et al. Investigating the possible mechanisms involved in adenosine preconditioning-induced cardioprotection in rats. Cardiovasc Ther. 2018;36(3):e12328.
Singh, L., Virdi, J. K., Maslov, L. N., Singh, N., & Jaggi, A. S. (2018). Investigating the possible mechanisms involved in adenosine preconditioning-induced cardioprotection in rats. Cardiovascular Therapeutics, 36(3), e12328. https://doi.org/10.1111/1755-5922.12328
Singh L, et al. Investigating the Possible Mechanisms Involved in Adenosine Preconditioning-induced Cardioprotection in Rats. Cardiovasc Ther. 2018;36(3):e12328. PubMed PMID: 29604187.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Investigating the possible mechanisms involved in adenosine preconditioning-induced cardioprotection in rats. AU - Singh,Lovedeep, AU - Virdi,Jasleen K, AU - Maslov,Leonid N, AU - Singh,Nirmal, AU - Jaggi,Amteshwar S, Y1 - 2018/04/19/ PY - 2017/12/05/received PY - 2018/02/23/revised PY - 2018/03/24/accepted PY - 2018/4/1/pubmed PY - 2018/9/28/medline PY - 2018/4/1/entrez KW - adenosine KW - cardioprotection KW - leukotrienes KW - nitric oxide SP - e12328 EP - e12328 JF - Cardiovascular therapeutics JO - Cardiovasc Ther VL - 36 IS - 3 N2 - BACKGROUND: Adenosine is a breakdown product of adenosine triphosphate and plays an important role in pharmacological preconditioning. The cardioprotective effects of adenosine preconditioning are well established. However, the possible mechanisms need to be explored. AIM: This study was aimed to investigate the possible mechanisms involved in adenosine preconditioning-induced cardioprotection in rats. METHODS: Rat heart was isolated and perfused on Langendorff apparatus. Global ischemia for 30 minutes followed by reperfusion for 120 minutes was employed to produce myocardial injury. Myocardial injury was assessed by measuring myocardial infarct size, release of lactate dehydrogenase (LDH) and creatine kinase (CK) in the coronary effluent and hemodynamic parameters including left ventricular developed pressure (LVDP), dp/dtmax, and dp/dtmin . Serum nitrite levels were measured as an index of nitric oxide release in blood. RESULTS: Adenosine (4 mg/kg) preconditioning significantly decreased ischemia-reperfusion-induced increase in LDH, CK release, infarct size, improved LVDP, dp/dtmax and dp/dtmin, and increased serum nitrite levels. Pretreatment with L-NAME, a specific NOS inhibitor, (5 mg/kg) and montelukast, leukotriene receptor antagonist, (10 mg/kg) significantly abrogated the cardioprotective effect of adenosine preconditioning. However, seratrodast, thromboxane A2 antagonist, (15 mg/kg) had no effect on adenosine-induced cardioprotection. Sodium nitroprusside (SNP) preconditioning also produced cardioprotective effects. However, caffeine (20 mg/kg) (adenosine receptor blocker) and seratrodast (15 mg/kg) had no effect on SNP-induced cardioprotection. Administration of montelukast abrogated the cardioprotective effects of SNP preconditioning-induced cardioprotection. CONCLUSION: Adenosine preconditioning may increase the release of nitric oxide, which in turn may increase the release of cysteinyl leukotrienes to confer cardioprotection. SN - 1755-5922 UR - https://www.unboundmedicine.com/medline/citation/29604187/Investigating_the_possible_mechanisms_involved_in_adenosine_preconditioning_induced_cardioprotection_in_rats_ L2 - https://doi.org/10.1111/1755-5922.12328 DB - PRIME DP - Unbound Medicine ER -