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A two dose immunization with an inactivated reassortant H5N2 virus protects chickens against lethal challenge with homologous 2.3.2.1 clade and heterologous 2.2 clade highly pathogenic avian influenza H5N1 viruses.
Vet Microbiol. 2018 Apr; 217:149-157.VM

Abstract

The present study was aimed at generating a reassortant vaccine candidate virus with clade 2.3.2.1 Hemagglutinin (HA) and its evaluation in a challenge study for protection against homologous (2.3.2.1 clade) and heterologous (2.2 clade) highly pathogenic avian influenza (HPAI) H5N1 viruses. Plasmid-based reverse genetics technique was used to rescue a 5 + 3 reassortant H5N2 strain containing the modified HA of H5N1 (clade 2.3.2.1), the Neuraminidase (NA) of H9N2, the Matrix (M) of H5N1 and the internal genes of A/WSN/33 H1N1. In addition, another 6 + 2 reassortant virus containing modified HA from H5N1 (clade 2.3.2.1), the NA from H9N2 and the internal genes of A/WSN/33 H1N1 was also rescued. The 5 + 3 reassortant H5N2 virus could grow to a higher titer in both MDCK cells and chicken eggs compared to the 6 + 2 reassortant H5N2 virus. The vaccine containing the inactivated 5 + 3 reassortant H5N2 virus was used in a two-dose immunization regime which protected specific pathogen free (SPF) chickens against two repeated challenges with homologous 2.3.2.1 clade and heterologous 2.2 clade HPAI H5N1 viruses. The 5 + 3 reassortant H5N2 virus based on clade 2.3.2.1 generated in this study can be effective in protecting chickens in the case of an outbreak caused by antigenically different clade 2.2 HPAI H5N1 viruses and opens the way to explore its applicability as potential vaccine candidate especially in the Asian countries reporting these clades frequently. The study also indicates that sequential immunization can broaden protection level against antigenically diverse strains of H5N1 viruses.

Authors+Show Affiliations

ICAR- National Institute of High Security Animal Diseases, Bhopal, Madhya Pradesh, India; Immunology Section, ICAR - Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India.ICAR- National Institute of High Security Animal Diseases, Bhopal, Madhya Pradesh, India.ICAR- National Institute of High Security Animal Diseases, Bhopal, Madhya Pradesh, India.ICAR- National Institute of High Security Animal Diseases, Bhopal, Madhya Pradesh, India.ICAR- National Institute of High Security Animal Diseases, Bhopal, Madhya Pradesh, India.ICAR- National Institute of High Security Animal Diseases, Bhopal, Madhya Pradesh, India.ICAR- National Institute of High Security Animal Diseases, Bhopal, Madhya Pradesh, India.ICAR- National Institute of High Security Animal Diseases, Bhopal, Madhya Pradesh, India.ICAR- National Institute of High Security Animal Diseases, Bhopal, Madhya Pradesh, India.ICAR- National Institute of High Security Animal Diseases, Bhopal, Madhya Pradesh, India. Electronic address: sandeep.bhatia1@icar.gov.in.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29615248

Citation

Bhat, Sushant, et al. "A Two Dose Immunization With an Inactivated Reassortant H5N2 Virus Protects Chickens Against Lethal Challenge With Homologous 2.3.2.1 Clade and Heterologous 2.2 Clade Highly Pathogenic Avian Influenza H5N1 Viruses." Veterinary Microbiology, vol. 217, 2018, pp. 149-157.
Bhat S, Sood R, Shukla S, et al. A two dose immunization with an inactivated reassortant H5N2 virus protects chickens against lethal challenge with homologous 2.3.2.1 clade and heterologous 2.2 clade highly pathogenic avian influenza H5N1 viruses. Vet Microbiol. 2018;217:149-157.
Bhat, S., Sood, R., Shukla, S., Khandia, R., Pateriya, A. K., Kumar, N., Singh, V. K., Kalaiyarasu, S., Kumar, M., & Bhatia, S. (2018). A two dose immunization with an inactivated reassortant H5N2 virus protects chickens against lethal challenge with homologous 2.3.2.1 clade and heterologous 2.2 clade highly pathogenic avian influenza H5N1 viruses. Veterinary Microbiology, 217, 149-157. https://doi.org/10.1016/j.vetmic.2018.03.004
Bhat S, et al. A Two Dose Immunization With an Inactivated Reassortant H5N2 Virus Protects Chickens Against Lethal Challenge With Homologous 2.3.2.1 Clade and Heterologous 2.2 Clade Highly Pathogenic Avian Influenza H5N1 Viruses. Vet Microbiol. 2018;217:149-157. PubMed PMID: 29615248.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A two dose immunization with an inactivated reassortant H5N2 virus protects chickens against lethal challenge with homologous 2.3.2.1 clade and heterologous 2.2 clade highly pathogenic avian influenza H5N1 viruses. AU - Bhat,Sushant, AU - Sood,Richa, AU - Shukla,Shweta, AU - Khandia,Rekha, AU - Pateriya,Atul Kumar, AU - Kumar,Naveen, AU - Singh,Vikas Kumar, AU - Kalaiyarasu,Semmannan, AU - Kumar,Manoj, AU - Bhatia,Sandeep, Y1 - 2018/03/13/ PY - 2017/12/03/received PY - 2018/03/08/revised PY - 2018/03/08/accepted PY - 2018/4/5/entrez PY - 2018/4/5/pubmed PY - 2018/11/21/medline KW - 2.3.2.1 Clade KW - Antibody titer KW - Avian influenza KW - HPAI H5N1 KW - High growth KW - Reverse genetics KW - Vaccine KW - Virus shedding SP - 149 EP - 157 JF - Veterinary microbiology JO - Vet. Microbiol. VL - 217 N2 - The present study was aimed at generating a reassortant vaccine candidate virus with clade 2.3.2.1 Hemagglutinin (HA) and its evaluation in a challenge study for protection against homologous (2.3.2.1 clade) and heterologous (2.2 clade) highly pathogenic avian influenza (HPAI) H5N1 viruses. Plasmid-based reverse genetics technique was used to rescue a 5 + 3 reassortant H5N2 strain containing the modified HA of H5N1 (clade 2.3.2.1), the Neuraminidase (NA) of H9N2, the Matrix (M) of H5N1 and the internal genes of A/WSN/33 H1N1. In addition, another 6 + 2 reassortant virus containing modified HA from H5N1 (clade 2.3.2.1), the NA from H9N2 and the internal genes of A/WSN/33 H1N1 was also rescued. The 5 + 3 reassortant H5N2 virus could grow to a higher titer in both MDCK cells and chicken eggs compared to the 6 + 2 reassortant H5N2 virus. The vaccine containing the inactivated 5 + 3 reassortant H5N2 virus was used in a two-dose immunization regime which protected specific pathogen free (SPF) chickens against two repeated challenges with homologous 2.3.2.1 clade and heterologous 2.2 clade HPAI H5N1 viruses. The 5 + 3 reassortant H5N2 virus based on clade 2.3.2.1 generated in this study can be effective in protecting chickens in the case of an outbreak caused by antigenically different clade 2.2 HPAI H5N1 viruses and opens the way to explore its applicability as potential vaccine candidate especially in the Asian countries reporting these clades frequently. The study also indicates that sequential immunization can broaden protection level against antigenically diverse strains of H5N1 viruses. SN - 1873-2542 UR - https://www.unboundmedicine.com/medline/citation/29615248/A_two_dose_immunization_with_an_inactivated_reassortant_H5N2_virus_protects_chickens_against_lethal_challenge_with_homologous_2_3_2_1_clade_and_heterologous_2_2_clade_highly_pathogenic_avian_influenza_H5N1_viruses_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(17)31284-1 DB - PRIME DP - Unbound Medicine ER -