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Molecular Cytogenetic Characterization of New Wheat-Dasypyrum breviaristatum Introgression Lines for Improving Grain Quality of Wheat.
Front Plant Sci. 2018; 9:365.FP

Abstract

As an important relative of wheat (Triticum aestivum L), Dasypyrum breviaristatum contains novel high molecular weight glutenin subunits (HMW-GSs) encoded by Glu-1Vb genes. We identified new wheat-D. breviaristatum chromosome introgression lines including chromosomes 1Vb and 1VbL.5VbL by fluorescence in situ hybridization (FISH) combined with molecular markers. We found that chromosome changes occurred in the wheat-D. breviaristatum introgression lines and particularly induced the deletion of 5BS terminal repeats and formation of a new type of 5B-7B reciprocal translocation. The results imply that the D. breviaristatum chromosome 1Vb may contain genes which induce chromosomal recombination in wheat background. Ten putative high molecular weight glutenin subunit (HMW-GS) genes from D. breviaristatum and wheat-D. breviaristatum introgression lines were isolated. The lengths of the HMW-GS genes in Dasypyrum were significantly shorter than typical HMW-GS of common wheat. A new y-type HMW-GS gene, named Glu-Vb1y, was characterized in wheat-D. breviaristatum 1Vb introgression lines. The new wheat-D. breviaristatum germplasm displayed reduced plant height, increased tillers and superior grain protein and gluten contents, improved gluten performance index. The results showed considerable potential for utilization of D. breviaristatum chromosome 1Vb segments in future wheat improvement.

Authors+Show Affiliations

School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China. Center of Informational Biology, University of Electronic Science and Technology of China, Chengdu, China.School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China. Center of Informational Biology, University of Electronic Science and Technology of China, Chengdu, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29616071

Citation

Wang, Hongjin, et al. "Molecular Cytogenetic Characterization of New Wheat-Dasypyrum Breviaristatum Introgression Lines for Improving Grain Quality of Wheat." Frontiers in Plant Science, vol. 9, 2018, p. 365.
Wang H, Zhang H, Li B, et al. Molecular Cytogenetic Characterization of New Wheat-Dasypyrum breviaristatum Introgression Lines for Improving Grain Quality of Wheat. Front Plant Sci. 2018;9:365.
Wang, H., Zhang, H., Li, B., Yu, Z., Li, G., Zhang, J., & Yang, Z. (2018). Molecular Cytogenetic Characterization of New Wheat-Dasypyrum breviaristatum Introgression Lines for Improving Grain Quality of Wheat. Frontiers in Plant Science, 9, 365. https://doi.org/10.3389/fpls.2018.00365
Wang H, et al. Molecular Cytogenetic Characterization of New Wheat-Dasypyrum Breviaristatum Introgression Lines for Improving Grain Quality of Wheat. Front Plant Sci. 2018;9:365. PubMed PMID: 29616071.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular Cytogenetic Characterization of New Wheat-Dasypyrum breviaristatum Introgression Lines for Improving Grain Quality of Wheat. AU - Wang,Hongjin, AU - Zhang,Hongjun, AU - Li,Bin, AU - Yu,Zhihui, AU - Li,Guangrong, AU - Zhang,Jie, AU - Yang,Zujun, Y1 - 2018/03/19/ PY - 2017/10/26/received PY - 2018/03/05/accepted PY - 2018/4/5/entrez PY - 2018/4/5/pubmed PY - 2018/4/5/medline KW - Dasypyrum KW - FISH KW - glutenin KW - grain quality KW - wheat SP - 365 EP - 365 JF - Frontiers in plant science JO - Front Plant Sci VL - 9 N2 - As an important relative of wheat (Triticum aestivum L), Dasypyrum breviaristatum contains novel high molecular weight glutenin subunits (HMW-GSs) encoded by Glu-1Vb genes. We identified new wheat-D. breviaristatum chromosome introgression lines including chromosomes 1Vb and 1VbL.5VbL by fluorescence in situ hybridization (FISH) combined with molecular markers. We found that chromosome changes occurred in the wheat-D. breviaristatum introgression lines and particularly induced the deletion of 5BS terminal repeats and formation of a new type of 5B-7B reciprocal translocation. The results imply that the D. breviaristatum chromosome 1Vb may contain genes which induce chromosomal recombination in wheat background. Ten putative high molecular weight glutenin subunit (HMW-GS) genes from D. breviaristatum and wheat-D. breviaristatum introgression lines were isolated. The lengths of the HMW-GS genes in Dasypyrum were significantly shorter than typical HMW-GS of common wheat. A new y-type HMW-GS gene, named Glu-Vb1y, was characterized in wheat-D. breviaristatum 1Vb introgression lines. The new wheat-D. breviaristatum germplasm displayed reduced plant height, increased tillers and superior grain protein and gluten contents, improved gluten performance index. The results showed considerable potential for utilization of D. breviaristatum chromosome 1Vb segments in future wheat improvement. SN - 1664-462X UR - https://www.unboundmedicine.com/medline/citation/29616071/Molecular_Cytogenetic_Characterization_of_New_Wheat_Dasypyrum_breviaristatum_Introgression_Lines_for_Improving_Grain_Quality_of_Wheat_ L2 - https://doi.org/10.3389/fpls.2018.00365 DB - PRIME DP - Unbound Medicine ER -
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