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Differential Expression of P450 Genes and nAChR Subunits Associated With Imidacloprid Resistance in Laodelphax striatellus (Hemiptera: Delphacidae).
J Econ Entomol. 2018 05 28; 111(3):1382-1387.JE

Abstract

Imidacloprid is a key insecticide used for controlling sucking insect pests, including the small brown planthopper (Laodelphax striatellus, Fallén) (Hemiptera: Delphacidae), an important agricultural pest of rice. A strain of L. striatellus (YN-ILR) developed 21-fold resistance when selected with imidacloprid on a susceptible YN strain. An in vitro study on piperonyl butoxide synergism indicated that enhanced detoxification mediated by cytochrome P450s contributed to imidacloprid resistance to some extent, and multiple P450 genes showed altered expression in the imidacloprid-resistant YN-ILR strain compared with the susceptible YN strain (CYP425B1-CYP6BD10 had 1.51- to 11.45-fold higher expression, CYP4CE2-CYP4DD1V2 had 0.12- to 0.57-fold lower expression). While there were no mutations in target nicotinic acetylcholine receptor (nAChR) genes, subunits of Lsα1, Lsβ1, and Lsβ3 in the YN-ILR strain showed 3.86-, 4.39-, and 2.59-fold higher expression and Lsa8 displayed 0.38-fold lower expression than the YN strain. Moreover, 21-fold moderate imidacloprid resistance in individuals of L. striatellus did not produce a fitness cost. The findings suggest that L. striatellus has the capacity to develop resistance to imidacloprid through P450 detoxification and potential target nAChR expression changes, and moderate imidacloprid resistance was not associated with a fitness cost.

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Authors+Show Affiliations

Zhang Y
Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Nanjing, China.
Liu B
Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Nanjing, China.
Zhang Z
Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Nanjing, China.
Wang L
Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Nanjing, China.
Guo H
Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Nanjing, China.
Li Z
Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai, China.
He P
State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang, China.
Liu Z
Key Laboratory of Monitoring and Management of Plant Disease and Insects, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, China.
Fang J
Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Nanjing, China.

MeSH

AnimalsCytochrome P-450 Enzyme SystemFemaleGene ExpressionHemipteraInsect ProteinsInsecticide ResistanceInsecticidesMaleNeonicotinoidsNitro CompoundsNymphReceptors, Nicotinic

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29617902

Citation

Zhang, Yueliang, et al. "Differential Expression of P450 Genes and nAChR Subunits Associated With Imidacloprid Resistance in Laodelphax Striatellus (Hemiptera: Delphacidae)." Journal of Economic Entomology, vol. 111, no. 3, 2018, pp. 1382-1387.
Zhang Y, Liu B, Zhang Z, et al. Differential Expression of P450 Genes and nAChR Subunits Associated With Imidacloprid Resistance in Laodelphax striatellus (Hemiptera: Delphacidae). J Econ Entomol. 2018;111(3):1382-1387.
Zhang, Y., Liu, B., Zhang, Z., Wang, L., Guo, H., Li, Z., He, P., Liu, Z., & Fang, J. (2018). Differential Expression of P450 Genes and nAChR Subunits Associated With Imidacloprid Resistance in Laodelphax striatellus (Hemiptera: Delphacidae). Journal of Economic Entomology, 111(3), 1382-1387. https://doi.org/10.1093/jee/toy051
Zhang Y, et al. Differential Expression of P450 Genes and nAChR Subunits Associated With Imidacloprid Resistance in Laodelphax Striatellus (Hemiptera: Delphacidae). J Econ Entomol. 2018 05 28;111(3):1382-1387. PubMed PMID: 29617902.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential Expression of P450 Genes and nAChR Subunits Associated With Imidacloprid Resistance in Laodelphax striatellus (Hemiptera: Delphacidae). AU - Zhang,Yueliang, AU - Liu,Baosheng, AU - Zhang,Zhichun, AU - Wang,Lihua, AU - Guo,Huifang, AU - Li,Zhong, AU - He,Peng, AU - Liu,Zewen, AU - Fang,Jichao, PY - 2017/09/25/received PY - 2018/4/5/pubmed PY - 2018/12/13/medline PY - 2018/4/5/entrez SP - 1382 EP - 1387 JF - Journal of economic entomology JO - J Econ Entomol VL - 111 IS - 3 N2 - Imidacloprid is a key insecticide used for controlling sucking insect pests, including the small brown planthopper (Laodelphax striatellus, Fallén) (Hemiptera: Delphacidae), an important agricultural pest of rice. A strain of L. striatellus (YN-ILR) developed 21-fold resistance when selected with imidacloprid on a susceptible YN strain. An in vitro study on piperonyl butoxide synergism indicated that enhanced detoxification mediated by cytochrome P450s contributed to imidacloprid resistance to some extent, and multiple P450 genes showed altered expression in the imidacloprid-resistant YN-ILR strain compared with the susceptible YN strain (CYP425B1-CYP6BD10 had 1.51- to 11.45-fold higher expression, CYP4CE2-CYP4DD1V2 had 0.12- to 0.57-fold lower expression). While there were no mutations in target nicotinic acetylcholine receptor (nAChR) genes, subunits of Lsα1, Lsβ1, and Lsβ3 in the YN-ILR strain showed 3.86-, 4.39-, and 2.59-fold higher expression and Lsa8 displayed 0.38-fold lower expression than the YN strain. Moreover, 21-fold moderate imidacloprid resistance in individuals of L. striatellus did not produce a fitness cost. The findings suggest that L. striatellus has the capacity to develop resistance to imidacloprid through P450 detoxification and potential target nAChR expression changes, and moderate imidacloprid resistance was not associated with a fitness cost. SN - 1938-291X UR - https://www.unboundmedicine.com/medline/citation/29617902/Differential_Expression_of_P450_Genes_and_nAChR_Subunits_Associated_With_Imidacloprid_Resistance_in_Laodelphax_striatellus__Hemiptera:_Delphacidae__ DB - PRIME DP - Unbound Medicine ER -