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Effects of single administration of apomorphine on memory and monoamine metabolism: A dose related study.
Pak J Pharm Sci 2018; 31(2):439-445PJ

Abstract

In the present study, we have monitored dose dependent effects of apomorphine on learning and memory. Behavioral sensitization and craving, which develop upon repeated treatment with dopamine receptor agonist apomorphine, are major limitations of the therapeutic use of apomorphine in Parkinson's patients. Effects of single (intraperitoneal) injection of apomorphine at different doses (i.e., 0.5, 1.0, & 2.0 mg/ml/kg) on locomotion in a familiar environment (Skinner's box) and memory in Morris water maze were investigated. Results show significantly enhanced activity in Skinner's box in a dose dependant manner. Low dose (0.5 mg/ml/kg) of apomorphine impaired both short- as well as long-term memory while both high and moderate doses of the drug (1.0, & 2.0 mg/ml/kg) enhanced the cognitive profile in rats. However, the memory-enhancing effects of apomorphine at moderate (1.0 mg/ml/kg) dose were more pronounced as compared to high (2.0 mg/ml/kg) dose of the drug. Rats were decapitated on day 2. Whole brains of rats were collected and stored at -70°C. Biogenic amines (i.e., 5-Hydroxytryptamine; 5-HT and dopamine) and metabolites (i.e., Dihydroxyphenylacetic acid; DOPAC, Homovanillic acid; HVA & 5-Hydroxyindoleacetic acid; 5HIAA) were estimated by reverse phase High Performance Liquid Chromatography with electrochemical detector (HPLC-EC). Both low (0.5mg/ml/kg) as well as moderate (1.0mg/ml/kg) dose of apomorphine increased levels of dopamine, DOPAC, HVA, 5-HT and 5-HIAA. Whereas, high (4.0 mg/kg) dose of apomorphine increased levels of dopamine, DOPAC and HVA, while decreased 5-HT and 5-HIAA levels. Results would be helpful in elucidating memory enhancing effects of apomorphine at different doses and its implication for extending therapeutics in cognitive disorders.

Authors+Show Affiliations

Neurochemistry and Biochemical Neuropharmacology Research Unit, Department of Biochemistry, University of Karachi, Karachi, Pakistan.Neurochemistry and Biochemical Neuropharmacology Research Unit, Department of Biochemistry, University of Karachi, Karachi, Pakistan.Neurochemistry and Biochemical Neuropharmacology Research Unit, Department of Biochemistry, University of Karachi, Karachi, Pakistan / Neuroscience Research Laboratory, Dr. Panjwani Center for Molecular Medicine and Drug Research, University of Karachi, Karachi, Pakistan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29618432

Citation

Ikram, Huma, et al. "Effects of Single Administration of Apomorphine On Memory and Monoamine Metabolism: a Dose Related Study." Pakistan Journal of Pharmaceutical Sciences, vol. 31, no. 2, 2018, pp. 439-445.
Ikram H, Zakir R, Haleem DJ. Effects of single administration of apomorphine on memory and monoamine metabolism: A dose related study. Pak J Pharm Sci. 2018;31(2):439-445.
Ikram, H., Zakir, R., & Haleem, D. J. (2018). Effects of single administration of apomorphine on memory and monoamine metabolism: A dose related study. Pakistan Journal of Pharmaceutical Sciences, 31(2), pp. 439-445.
Ikram H, Zakir R, Haleem DJ. Effects of Single Administration of Apomorphine On Memory and Monoamine Metabolism: a Dose Related Study. Pak J Pharm Sci. 2018;31(2):439-445. PubMed PMID: 29618432.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of single administration of apomorphine on memory and monoamine metabolism: A dose related study. AU - Ikram,Huma, AU - Zakir,Rumaisa, AU - Haleem,Darakhshan Jabeen, PY - 2018/4/6/entrez PY - 2018/4/6/pubmed PY - 2019/8/3/medline SP - 439 EP - 445 JF - Pakistan journal of pharmaceutical sciences JO - Pak J Pharm Sci VL - 31 IS - 2 N2 - In the present study, we have monitored dose dependent effects of apomorphine on learning and memory. Behavioral sensitization and craving, which develop upon repeated treatment with dopamine receptor agonist apomorphine, are major limitations of the therapeutic use of apomorphine in Parkinson's patients. Effects of single (intraperitoneal) injection of apomorphine at different doses (i.e., 0.5, 1.0, & 2.0 mg/ml/kg) on locomotion in a familiar environment (Skinner's box) and memory in Morris water maze were investigated. Results show significantly enhanced activity in Skinner's box in a dose dependant manner. Low dose (0.5 mg/ml/kg) of apomorphine impaired both short- as well as long-term memory while both high and moderate doses of the drug (1.0, & 2.0 mg/ml/kg) enhanced the cognitive profile in rats. However, the memory-enhancing effects of apomorphine at moderate (1.0 mg/ml/kg) dose were more pronounced as compared to high (2.0 mg/ml/kg) dose of the drug. Rats were decapitated on day 2. Whole brains of rats were collected and stored at -70°C. Biogenic amines (i.e., 5-Hydroxytryptamine; 5-HT and dopamine) and metabolites (i.e., Dihydroxyphenylacetic acid; DOPAC, Homovanillic acid; HVA & 5-Hydroxyindoleacetic acid; 5HIAA) were estimated by reverse phase High Performance Liquid Chromatography with electrochemical detector (HPLC-EC). Both low (0.5mg/ml/kg) as well as moderate (1.0mg/ml/kg) dose of apomorphine increased levels of dopamine, DOPAC, HVA, 5-HT and 5-HIAA. Whereas, high (4.0 mg/kg) dose of apomorphine increased levels of dopamine, DOPAC and HVA, while decreased 5-HT and 5-HIAA levels. Results would be helpful in elucidating memory enhancing effects of apomorphine at different doses and its implication for extending therapeutics in cognitive disorders. SN - 1011-601X UR - https://www.unboundmedicine.com/medline/citation/29618432/Effects_of_single_administration_of_apomorphine_on_memory_and_monoamine_metabolism:_A_dose_related_study_ L2 - https://medlineplus.gov/memory.html DB - PRIME DP - Unbound Medicine ER -