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Enhanced protection in mice induced by immunization with inactivated whole viruses compare to spike protein of middle east respiratory syndrome coronavirus.
Emerg Microbes Infect. 2018 Apr 04; 7(1):60.EM

Abstract

The persistent public health threat of infection with Middle East respiratory syndrome coronavirus (MERS-CoV) highlights the need for an effective and safe MERS-CoV vaccine. In this study, we prepared and vaccinated mice with either a Spike (S) protein or inactivated whole MERS-CoV (IV) with a combined adjuvant (alum+CpG) as a vaccine formulation. Similar levels of the anti-S protein IgG response and neutralizing activity were induced by both the S protein and IV vaccines. In addition, immune responses against three other structural proteins, the envelope (E), membrane (M), and nucleocapsid (N) proteins, were also detected in sera of mice that received IV. No antigen-specific T-cell immunity was detected after vaccination based on the interferon-γ ELISpot assay. Mice were transduced with Ad5-hDPP4 after the final immunization and were then challenged with MERS-CoV (1 × 105 plaque-forming units). Compared with the control group (adjuvant alone), mice immunized with the S protein or IV showed slightly lower pathological damage in the lung, as well as reduced antigen expression and lung virus titers. Mice that received IV formulations also showed increased protective immunity (almost no live virus was isolated from the lung). In conclusion, our data indicate that immunization with our IV formulation induced enhanced protection in mice compared to immunization with the S protein against MERS-CoV, which should be further tested in camels and clinical trials.

Authors+Show Affiliations

MOH Key Laboratory of Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.MOH Key Laboratory of Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China. Department of Pathogenic Biology, Hebei Medical University, Shijiazhuang, Heibei Province, 050017, China.Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS) & Comparative Medicine Center, Peking Union Medical Collage (PUMC), MOH Key Laboratory of Human Disease Comparative Medicine, Beijing, 100021, China.MOH Key Laboratory of Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.MOH Key Laboratory of Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.MOH Key Laboratory of Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS) & Comparative Medicine Center, Peking Union Medical Collage (PUMC), MOH Key Laboratory of Human Disease Comparative Medicine, Beijing, 100021, China.MOH Key Laboratory of Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS) & Comparative Medicine Center, Peking Union Medical Collage (PUMC), MOH Key Laboratory of Human Disease Comparative Medicine, Beijing, 100021, China.MOH Key Laboratory of Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China. tanwj28@163.com.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

29618723

Citation

Deng, Yao, et al. "Enhanced Protection in Mice Induced By Immunization With Inactivated Whole Viruses Compare to Spike Protein of Middle East Respiratory Syndrome Coronavirus." Emerging Microbes & Infections, vol. 7, no. 1, 2018, p. 60.
Deng Y, Lan J, Bao L, et al. Enhanced protection in mice induced by immunization with inactivated whole viruses compare to spike protein of middle east respiratory syndrome coronavirus. Emerg Microbes Infect. 2018;7(1):60.
Deng, Y., Lan, J., Bao, L., Huang, B., Ye, F., Chen, Y., Yao, Y., Wang, W., Qin, C., & Tan, W. (2018). Enhanced protection in mice induced by immunization with inactivated whole viruses compare to spike protein of middle east respiratory syndrome coronavirus. Emerging Microbes & Infections, 7(1), 60. https://doi.org/10.1038/s41426-018-0056-7
Deng Y, et al. Enhanced Protection in Mice Induced By Immunization With Inactivated Whole Viruses Compare to Spike Protein of Middle East Respiratory Syndrome Coronavirus. Emerg Microbes Infect. 2018 Apr 4;7(1):60. PubMed PMID: 29618723.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enhanced protection in mice induced by immunization with inactivated whole viruses compare to spike protein of middle east respiratory syndrome coronavirus. AU - Deng,Yao, AU - Lan,Jiaming, AU - Bao,Linlin, AU - Huang,Baoying, AU - Ye,Fei, AU - Chen,Yingzhu, AU - Yao,Yanfeng, AU - Wang,Wenling, AU - Qin,Chuan, AU - Tan,Wenjie, Y1 - 2018/04/04/ PY - 2017/09/01/received PY - 2018/02/11/accepted PY - 2018/01/29/revised PY - 2018/4/6/entrez PY - 2018/4/6/pubmed PY - 2018/11/16/medline SP - 60 EP - 60 JF - Emerging microbes & infections JO - Emerg Microbes Infect VL - 7 IS - 1 N2 - The persistent public health threat of infection with Middle East respiratory syndrome coronavirus (MERS-CoV) highlights the need for an effective and safe MERS-CoV vaccine. In this study, we prepared and vaccinated mice with either a Spike (S) protein or inactivated whole MERS-CoV (IV) with a combined adjuvant (alum+CpG) as a vaccine formulation. Similar levels of the anti-S protein IgG response and neutralizing activity were induced by both the S protein and IV vaccines. In addition, immune responses against three other structural proteins, the envelope (E), membrane (M), and nucleocapsid (N) proteins, were also detected in sera of mice that received IV. No antigen-specific T-cell immunity was detected after vaccination based on the interferon-γ ELISpot assay. Mice were transduced with Ad5-hDPP4 after the final immunization and were then challenged with MERS-CoV (1 × 105 plaque-forming units). Compared with the control group (adjuvant alone), mice immunized with the S protein or IV showed slightly lower pathological damage in the lung, as well as reduced antigen expression and lung virus titers. Mice that received IV formulations also showed increased protective immunity (almost no live virus was isolated from the lung). In conclusion, our data indicate that immunization with our IV formulation induced enhanced protection in mice compared to immunization with the S protein against MERS-CoV, which should be further tested in camels and clinical trials. SN - 2222-1751 UR - https://www.unboundmedicine.com/medline/citation/29618723/Enhanced_protection_in_mice_induced_by_immunization_with_inactivated_whole_viruses_compare_to_spike_protein_of_middle_east_respiratory_syndrome_coronavirus_ L2 - https://www.tandfonline.com/doi/full/10.1038/s41426-018-0056-7 DB - PRIME DP - Unbound Medicine ER -