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Poligoni Multiflori Radix enhances osteoblast formation and reduces osteoclast differentiation.
Int J Mol Med. 2018 Jul; 42(1):331-345.IJ

Abstract

Poligoni Multiflori Radix (PMR) is a traditional Korean medicinal herb that is known to have various pharmacological effects, including antihyperlipidemic, anticancer, and anti‑inflammatory effects. However, the effects of PMR on bone metabolism have not been elucidated to date. The present study aimed to investigate the in vitro and in vivo effect of PMR water extract on the regulation of osteoblast and osteoclast activity. Effects of PMR water extract on receptor activator of nuclear factor‑kB ligand (RANKL)‑induced osteoclast differentiation and survival of mouse bone marrow macrophages (BMMs) obtained from femurs were investigated by tartrate‑acid resistant acid phosphatase (TRAP)‑positive cells and XTT assay. Expression of osteoclast‑related genes was assayed by western blot analysis and reverse transcription‑quantitative polymerase chain reaction. Additionally, the effects of PMR water extract on osteoblastic proliferation and differentiation were investigated by alkaline phosphatase (ALP) activity assay, alizarin red staining, and levels of mRNA encoding known osteoblast markers. Furthermore, the effects of PMR water extract on lipopolysaccharide (LPS)‑induced bone loss were examined in a mouse model. PMR inhibited RANKL‑induced osteoclast differentiation of BMMs in a dose‑dependent manner without significant cytotoxicity, and suppressed expression of the main osteoclast differentiation markers Fos proto‑oncogene and nuclear factor of activated T‑cell. In addition, PMR decreased the mRNA expression levels of NFATc1 target genes, including TRAP, osteoclast‑associated receptor, ATPase H+ transporting, lysosomal 38 kDa V0 subunit d2, and Cathepsin K. These inhibitory effects were mediated by the p38 and extracellular signal‑regulated kinase/nuclear factor‑κB pathway. Simultaneously, PMR enhanced the differentiation of primary osteoblasts, and increased the mRNA expression of runt‑related transcription factor 2, ALP, osterix, and osteocalcin. Notably, PMR improved LPS‑induced trabecular bone loss in mice. Collectively, the present findings demonstrated that PMR may regulate bone remodeling by reducing osteoclast differentiation and stimulating osteoblast formation. These results suggest that PMR may be used for the treatment of bone diseases, such as osteoporosis and rheumatoid arthritis.

Authors+Show Affiliations

Department of Herbal Medicine, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Wonkwang University, Iksan, Jeonbuk 54538, Republic of Korea.Department of Herbal Medicine, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Wonkwang University, Iksan, Jeonbuk 54538, Republic of Korea.Department of Herbal Medicine, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Wonkwang University, Iksan, Jeonbuk 54538, Republic of Korea.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29620250

Citation

Han, Sang-Yong, et al. "Poligoni Multiflori Radix Enhances Osteoblast Formation and Reduces Osteoclast Differentiation." International Journal of Molecular Medicine, vol. 42, no. 1, 2018, pp. 331-345.
Han SY, Lee KH, Kim YK. Poligoni Multiflori Radix enhances osteoblast formation and reduces osteoclast differentiation. Int J Mol Med. 2018;42(1):331-345.
Han, S. Y., Lee, K. H., & Kim, Y. K. (2018). Poligoni Multiflori Radix enhances osteoblast formation and reduces osteoclast differentiation. International Journal of Molecular Medicine, 42(1), 331-345. https://doi.org/10.3892/ijmm.2018.3603
Han SY, Lee KH, Kim YK. Poligoni Multiflori Radix Enhances Osteoblast Formation and Reduces Osteoclast Differentiation. Int J Mol Med. 2018;42(1):331-345. PubMed PMID: 29620250.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Poligoni Multiflori Radix enhances osteoblast formation and reduces osteoclast differentiation. AU - Han,Sang-Yong, AU - Lee,Kyung-Hee, AU - Kim,Yun-Kyung, Y1 - 2018/03/30/ PY - 2017/10/18/received PY - 2018/03/22/accepted PY - 2018/4/6/pubmed PY - 2018/9/25/medline PY - 2018/4/6/entrez SP - 331 EP - 345 JF - International journal of molecular medicine JO - Int J Mol Med VL - 42 IS - 1 N2 - Poligoni Multiflori Radix (PMR) is a traditional Korean medicinal herb that is known to have various pharmacological effects, including antihyperlipidemic, anticancer, and anti‑inflammatory effects. However, the effects of PMR on bone metabolism have not been elucidated to date. The present study aimed to investigate the in vitro and in vivo effect of PMR water extract on the regulation of osteoblast and osteoclast activity. Effects of PMR water extract on receptor activator of nuclear factor‑kB ligand (RANKL)‑induced osteoclast differentiation and survival of mouse bone marrow macrophages (BMMs) obtained from femurs were investigated by tartrate‑acid resistant acid phosphatase (TRAP)‑positive cells and XTT assay. Expression of osteoclast‑related genes was assayed by western blot analysis and reverse transcription‑quantitative polymerase chain reaction. Additionally, the effects of PMR water extract on osteoblastic proliferation and differentiation were investigated by alkaline phosphatase (ALP) activity assay, alizarin red staining, and levels of mRNA encoding known osteoblast markers. Furthermore, the effects of PMR water extract on lipopolysaccharide (LPS)‑induced bone loss were examined in a mouse model. PMR inhibited RANKL‑induced osteoclast differentiation of BMMs in a dose‑dependent manner without significant cytotoxicity, and suppressed expression of the main osteoclast differentiation markers Fos proto‑oncogene and nuclear factor of activated T‑cell. In addition, PMR decreased the mRNA expression levels of NFATc1 target genes, including TRAP, osteoclast‑associated receptor, ATPase H+ transporting, lysosomal 38 kDa V0 subunit d2, and Cathepsin K. These inhibitory effects were mediated by the p38 and extracellular signal‑regulated kinase/nuclear factor‑κB pathway. Simultaneously, PMR enhanced the differentiation of primary osteoblasts, and increased the mRNA expression of runt‑related transcription factor 2, ALP, osterix, and osteocalcin. Notably, PMR improved LPS‑induced trabecular bone loss in mice. Collectively, the present findings demonstrated that PMR may regulate bone remodeling by reducing osteoclast differentiation and stimulating osteoblast formation. These results suggest that PMR may be used for the treatment of bone diseases, such as osteoporosis and rheumatoid arthritis. SN - 1791-244X UR - https://www.unboundmedicine.com/medline/citation/29620250/Poligoni_Multiflori_Radix_enhances_osteoblast_formation_and_reduces_osteoclast_differentiation_ L2 - http://www.spandidos-publications.com/ijmm/42/1/331 DB - PRIME DP - Unbound Medicine ER -