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Genetic characterisation of Staphylococcus aureus isolated from milk and nasal samples of healthy cows in Tunisia: First report of ST97-t267-agrI-SCCmecV MRSA of bovine origin in Tunisia.
J Glob Antimicrob Resist. 2018 09; 14:161-165.JG

Abstract

OBJECTIVES

This study aimed to screen for and characterise methicillin-susceptible Staphylococcus aureus (MSSA) and methicillin-resistant S. aureus (MRSA) in nasal swabs and milk from healthy cows from different regions in Tunisia.

METHODS

A total of 141 Staphylococcus spp. isolates were recovered from milk and nasal samples of cows. S. aureus isolates were further characterised by determining their antimicrobial susceptibilities, genes encoding antimicrobial resistance and virulence factors, biofilm production, agr type and PFGE. spa and SCCmec typing and MLST were also performed for the MRSA isolate.

RESULTS

Twenty-seven isolates (19.1%) were identified as S. aureus, of which 26 were MSSA and 1 was MRSA. The MSSA isolates were resistant to penicillin (73.1%), fusidic acid (61.5%), clindamycin (34.6%) and erythromycin (34.6%). The MRSA isolate, from a milk sample, was resistant to cefoxitin, penicillin, fusidic acid, amikacin and clindamycin. Twenty-five isolates (92.6%) had at least one enterotoxin gene. Only four isolates (14.8%) were positive for the tsst-1 gene. Genes encoding the exfoliative toxins D and A were detected in 9 (33.3%) and 6 (22.2%) isolates, respectively. The single MRSA isolate and 22 MSSA isolates were biofilm-producers on Congo red agar plates. Twelve pulsotypes were identified amongst 25 MSSA isolates revealing the clonal diversity of these isolates; however, one MSSA isolate was identified as CC398. The MRSA isolate was PVL-negative and was typed as ST97-t267-agrI-SCCmecV.

CONCLUSION

Contamination of milk with S. aureus, especially enterotoxin- and TSST-1-positive strains, poses a potential public-health threat. This is the first report of MRSA of bovine origin in Tunisia.

Authors+Show Affiliations

Université de Tunis El Manar, Institut de la Recherche Vétérinaire de Tunisie, 20 rue Jebel Lakhdhar, Bab Saadoun, Tunis 1006, Tunisia; Université de la Manouba, Ecole Nationale de Médecine Vétérinaire de Sidi Thabet, Tunisia.Université de Tunis El Manar, Institut de la Recherche Vétérinaire de Tunisie, 20 rue Jebel Lakhdhar, Bab Saadoun, Tunis 1006, Tunisia. Electronic address: salahtoumi_mohamed@yahoo.com.Laboratory of Antimicrobial and Biocide Resistance, CIISA, Faculty of Veterinary Medicine, University of Lisbon, Avenida da Universidade Técnica, 1300-477 Lisbon, Portugal.Université de Tunis El Manar, Institut de la Recherche Vétérinaire de Tunisie, 20 rue Jebel Lakhdhar, Bab Saadoun, Tunis 1006, Tunisia.Université de la Manouba, Ecole Nationale de Médecine Vétérinaire de Sidi Thabet, Tunisia.Laboratory of Antimicrobial and Biocide Resistance, CIISA, Faculty of Veterinary Medicine, University of Lisbon, Avenida da Universidade Técnica, 1300-477 Lisbon, Portugal. Electronic address: cpomba@fmv.ulisboa.pt.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29621629

Citation

Khemiri, Monia, et al. "Genetic Characterisation of Staphylococcus Aureus Isolated From Milk and Nasal Samples of Healthy Cows in Tunisia: First Report of ST97-t267-agrI-SCCmecV MRSA of Bovine Origin in Tunisia." Journal of Global Antimicrobial Resistance, vol. 14, 2018, pp. 161-165.
Khemiri M, Abbassi MS, Couto N, et al. Genetic characterisation of Staphylococcus aureus isolated from milk and nasal samples of healthy cows in Tunisia: First report of ST97-t267-agrI-SCCmecV MRSA of bovine origin in Tunisia. J Glob Antimicrob Resist. 2018;14:161-165.
Khemiri, M., Abbassi, M. S., Couto, N., Mansouri, R., Hammami, S., & Pomba, C. (2018). Genetic characterisation of Staphylococcus aureus isolated from milk and nasal samples of healthy cows in Tunisia: First report of ST97-t267-agrI-SCCmecV MRSA of bovine origin in Tunisia. Journal of Global Antimicrobial Resistance, 14, 161-165. https://doi.org/10.1016/j.jgar.2018.03.013
Khemiri M, et al. Genetic Characterisation of Staphylococcus Aureus Isolated From Milk and Nasal Samples of Healthy Cows in Tunisia: First Report of ST97-t267-agrI-SCCmecV MRSA of Bovine Origin in Tunisia. J Glob Antimicrob Resist. 2018;14:161-165. PubMed PMID: 29621629.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic characterisation of Staphylococcus aureus isolated from milk and nasal samples of healthy cows in Tunisia: First report of ST97-t267-agrI-SCCmecV MRSA of bovine origin in Tunisia. AU - Khemiri,Monia, AU - Abbassi,Mohamed Salah, AU - Couto,Natacha, AU - Mansouri,Riadh, AU - Hammami,Salah, AU - Pomba,Constança, Y1 - 2018/04/03/ PY - 2017/05/24/received PY - 2018/01/13/revised PY - 2018/03/26/accepted PY - 2018/4/6/pubmed PY - 2019/9/29/medline PY - 2018/4/6/entrez KW - Antimicrobial resistance KW - ST97-t267-SCCmecV KW - Staphylococcus aureus KW - Virulotype SP - 161 EP - 165 JF - Journal of global antimicrobial resistance JO - J Glob Antimicrob Resist VL - 14 N2 - OBJECTIVES: This study aimed to screen for and characterise methicillin-susceptible Staphylococcus aureus (MSSA) and methicillin-resistant S. aureus (MRSA) in nasal swabs and milk from healthy cows from different regions in Tunisia. METHODS: A total of 141 Staphylococcus spp. isolates were recovered from milk and nasal samples of cows. S. aureus isolates were further characterised by determining their antimicrobial susceptibilities, genes encoding antimicrobial resistance and virulence factors, biofilm production, agr type and PFGE. spa and SCCmec typing and MLST were also performed for the MRSA isolate. RESULTS: Twenty-seven isolates (19.1%) were identified as S. aureus, of which 26 were MSSA and 1 was MRSA. The MSSA isolates were resistant to penicillin (73.1%), fusidic acid (61.5%), clindamycin (34.6%) and erythromycin (34.6%). The MRSA isolate, from a milk sample, was resistant to cefoxitin, penicillin, fusidic acid, amikacin and clindamycin. Twenty-five isolates (92.6%) had at least one enterotoxin gene. Only four isolates (14.8%) were positive for the tsst-1 gene. Genes encoding the exfoliative toxins D and A were detected in 9 (33.3%) and 6 (22.2%) isolates, respectively. The single MRSA isolate and 22 MSSA isolates were biofilm-producers on Congo red agar plates. Twelve pulsotypes were identified amongst 25 MSSA isolates revealing the clonal diversity of these isolates; however, one MSSA isolate was identified as CC398. The MRSA isolate was PVL-negative and was typed as ST97-t267-agrI-SCCmecV. CONCLUSION: Contamination of milk with S. aureus, especially enterotoxin- and TSST-1-positive strains, poses a potential public-health threat. This is the first report of MRSA of bovine origin in Tunisia. SN - 2213-7173 UR - https://www.unboundmedicine.com/medline/citation/29621629/Genetic_characterisation_of_Staphylococcus_aureus_isolated_from_milk_and_nasal_samples_of_healthy_cows_in_Tunisia:_First_report_of_ST97_t267_agrI_SCCmecV_MRSA_of_bovine_origin_in_Tunisia_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S2213-7165(18)30065-1 DB - PRIME DP - Unbound Medicine ER -