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Taurine Depletion Causes ipRGC Loss and Increases Light-Induced Photoreceptor Degeneration.
Invest Ophthalmol Vis Sci. 2018 Mar 01; 59(3):1396-1409.IO

Abstract

PURPOSE

To examine if light exposure exacerbates retinal neuronal loss induced by taurine depletion.

METHODS

Albino rats received β-alanine in the drinking water to induce taurine depletion. One month later, half of the animals were exposed to white light (3000 lux) continuously for 48 hours and the rest remained in normal environmental conditions. A control group of animals nontreated with β-alanine also was prepared, and half of them were exposed to light using the same protocol. All the animals were processed 2 months after the beginning of the experiment. Retinas were dissected as wholemounts and immunodetected with antibodies against Brn3a, melanopsin, S-opsin, and L-opsin to label different retinal populations: Brn3a+ retinal ganglion cells (RGCs) (image-forming RGCs), m+RGCs (non-image-forming RGCs), and S- and L/M-cones, respectively.

RESULTS

Light exposure did not affect the numbers of Brn3a+RGCs or m+RGCs but diminished the numbers of S- and L/M-cones and caused the appearance of rings devoid of cones, mainly in an "arciform" area in the superotemporal retina. Taurine depletion caused a diminution of all the studied populations, with m+RGCs the most affected, followed by S-cones. Light exposure under taurine depletion increased photoreceptor degeneration but did not seem to increase Brn3a+RGCs or m+RGCs loss.

CONCLUSIONS

Our results document that taurine is necessary for cell survival in the rat retina and even more under light-induced photoreceptor degeneration. Thus, taurine supplementation may help to prevent retinal degenerations, especially those that commence with S-cone degeneration or in which light may be an etiologic factor, such as inherited retinal degenerations, AMD, or glaucoma.

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Publisher Full Text (DOI)

Authors+Show Affiliations

García-Ayuso D
Departamento de Oftalmología, Facultad de Medicina, Universidad de Murcia, and Instituto Murciano de Investigación Biosanitaria Hospital Virgen de la Arrixaca (IMIB-Virgen de la Arrixaca), Murcia, Spain.
Di Pierdomenico J
Departamento de Oftalmología, Facultad de Medicina, Universidad de Murcia, and Instituto Murciano de Investigación Biosanitaria Hospital Virgen de la Arrixaca (IMIB-Virgen de la Arrixaca), Murcia, Spain.
Hadj-Said W
INSERM U968, Institut de la Vision, Paris, France. Sorbonne Universités, UPMC Université Paris 06, INSERM U968, CNRS UMR 7210, Institut de la Vision, Paris, France.
Marie M
INSERM U968, Institut de la Vision, Paris, France. Sorbonne Universités, UPMC Université Paris 06, INSERM U968, CNRS UMR 7210, Institut de la Vision, Paris, France.
Agudo-Barriuso M
Departamento de Oftalmología, Facultad de Medicina, Universidad de Murcia, and Instituto Murciano de Investigación Biosanitaria Hospital Virgen de la Arrixaca (IMIB-Virgen de la Arrixaca), Murcia, Spain.
Vidal-Sanz M
Departamento de Oftalmología, Facultad de Medicina, Universidad de Murcia, and Instituto Murciano de Investigación Biosanitaria Hospital Virgen de la Arrixaca (IMIB-Virgen de la Arrixaca), Murcia, Spain.
Picaud S
INSERM U968, Institut de la Vision, Paris, France. Sorbonne Universités, UPMC Université Paris 06, INSERM U968, CNRS UMR 7210, Institut de la Vision, Paris, France.
Villegas-Pérez MP
Departamento de Oftalmología, Facultad de Medicina, Universidad de Murcia, and Instituto Murciano de Investigación Biosanitaria Hospital Virgen de la Arrixaca (IMIB-Virgen de la Arrixaca), Murcia, Spain.

MeSH

AnimalsCell SurvivalDisease Models, AnimalLightPhotoreceptor Cells, VertebrateRatsRats, Sprague-DawleyRetinal DegenerationRetinal Ganglion CellsTaurinebeta-Alanine

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29625463

Citation

García-Ayuso, Diego, et al. "Taurine Depletion Causes ipRGC Loss and Increases Light-Induced Photoreceptor Degeneration." Investigative Ophthalmology & Visual Science, vol. 59, no. 3, 2018, pp. 1396-1409.
García-Ayuso D, Di Pierdomenico J, Hadj-Said W, et al. Taurine Depletion Causes ipRGC Loss and Increases Light-Induced Photoreceptor Degeneration. Invest Ophthalmol Vis Sci. 2018;59(3):1396-1409.
García-Ayuso, D., Di Pierdomenico, J., Hadj-Said, W., Marie, M., Agudo-Barriuso, M., Vidal-Sanz, M., Picaud, S., & Villegas-Pérez, M. P. (2018). Taurine Depletion Causes ipRGC Loss and Increases Light-Induced Photoreceptor Degeneration. Investigative Ophthalmology & Visual Science, 59(3), 1396-1409. https://doi.org/10.1167/iovs.17-23258
García-Ayuso D, et al. Taurine Depletion Causes ipRGC Loss and Increases Light-Induced Photoreceptor Degeneration. Invest Ophthalmol Vis Sci. 2018 Mar 1;59(3):1396-1409. PubMed PMID: 29625463.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Taurine Depletion Causes ipRGC Loss and Increases Light-Induced Photoreceptor Degeneration. AU - García-Ayuso,Diego, AU - Di Pierdomenico,Johnny, AU - Hadj-Said,Wahiba, AU - Marie,Mélanie, AU - Agudo-Barriuso,Marta, AU - Vidal-Sanz,Manuel, AU - Picaud,Serge, AU - Villegas-Pérez,María P, PY - 2018/4/7/entrez PY - 2018/4/7/pubmed PY - 2018/8/22/medline SP - 1396 EP - 1409 JF - Investigative ophthalmology & visual science JO - Invest Ophthalmol Vis Sci VL - 59 IS - 3 N2 - PURPOSE: To examine if light exposure exacerbates retinal neuronal loss induced by taurine depletion. METHODS: Albino rats received β-alanine in the drinking water to induce taurine depletion. One month later, half of the animals were exposed to white light (3000 lux) continuously for 48 hours and the rest remained in normal environmental conditions. A control group of animals nontreated with β-alanine also was prepared, and half of them were exposed to light using the same protocol. All the animals were processed 2 months after the beginning of the experiment. Retinas were dissected as wholemounts and immunodetected with antibodies against Brn3a, melanopsin, S-opsin, and L-opsin to label different retinal populations: Brn3a+ retinal ganglion cells (RGCs) (image-forming RGCs), m+RGCs (non-image-forming RGCs), and S- and L/M-cones, respectively. RESULTS: Light exposure did not affect the numbers of Brn3a+RGCs or m+RGCs but diminished the numbers of S- and L/M-cones and caused the appearance of rings devoid of cones, mainly in an "arciform" area in the superotemporal retina. Taurine depletion caused a diminution of all the studied populations, with m+RGCs the most affected, followed by S-cones. Light exposure under taurine depletion increased photoreceptor degeneration but did not seem to increase Brn3a+RGCs or m+RGCs loss. CONCLUSIONS: Our results document that taurine is necessary for cell survival in the rat retina and even more under light-induced photoreceptor degeneration. Thus, taurine supplementation may help to prevent retinal degenerations, especially those that commence with S-cone degeneration or in which light may be an etiologic factor, such as inherited retinal degenerations, AMD, or glaucoma. SN - 1552-5783 UR - https://www.unboundmedicine.com/medline/citation/29625463/Taurine_Depletion_Causes_ipRGC_Loss_and_Increases_Light_Induced_Photoreceptor_Degeneration_ DB - PRIME DP - Unbound Medicine ER -