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Differential responses to Cd stress induced by exogenous application of Cu, Zn or Ca in the medicinal plant Catharanthus roseus.
Ecotoxicol Environ Saf. 2018 Aug 15; 157:266-275.EE

Abstract

Cd(II) is one of the most widespread and toxic heavy metals and seriously threatens plant growth, furthermore negatively affecting human health. For survival from this metal stress, plants always fight with Cd(II) toxicity by themselves or using other external factors. The effects of second metals copper (Cu(II)), zinc (Zn(II)) and calcium (Ca(II)) on the Cd(II)-affected root morphology, Cd(II) translocation and metabolic responses in Catharanthus roseus were investigated under hydroponic conditions. We found that the Cd-stressed plants displayed the browning and rot root symptom, excess H2O2 content, lipid peroxidation and Cd(II) accumulation in plants. However, the supplement with second metals largely alleviated Cd-induced toxicity, including browning and rot roots, oxidative stress and internal Cd(II) accumulation. The amended effects at metabolic and transcriptional levels involved in different second metals share either common or divergent strategies. They commonly repressed Cd uptake and promoted Cd(II) translocation from roots to shoots with divergent mechanisms. High Zn(II) could activate MTs expression in roots, while Cu(II) or Ca(II) did not under Cd(II) stress condition. The presence of Ca(II) under Cd stress condition largely initiated occurrence of lateral roots. We then grouped a metabolic diagram integrating terpenoid indole alkaloid (TIA) accumulation and TIA pathway gene expression to elucidate the metabolic response of C. roseus to Cd(II) alone or combined with second metals. The treatment with 100 Cd(II) alone largely promoted accumulation of vinblastine, vindoline, catharanthine and loganin, whereas depressed or little changed the expression levels of genes detected here, compared to 0 Cd(II) control. In the presence of Cd(II), the supplement with second metals displayed specific effect on different alkaloid. Among them, the metal Ca(II) is especially beneficial for serpentine accumulation, Zn(II) mainly promoted tabersonine production. However, the addition of Cu(II) commonly depressed accumulation of most alkaloids detected here. Generally, we presented different mechanisms by which the second metals used to alleviate Cd (II) toxicity. This plant has potential application in phytoremediation of Cd(II), due to relatively substantial accumulation of biomass, as well as secondary metabolites TIAs used as pharmaceutical materials when facing Cd stress.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Chen Q
Center for Ecological Research, Northeast Forestry University, Harbin 150040, PR China.
Lu X
Center for Ecological Research, Northeast Forestry University, Harbin 150040, PR China.
Guo X
Key Laboratory of Plant Ecology, Northeast Forestry University, Harbin 150040, PR China.
Pan Y
Key Laboratory of Plant Ecology, Northeast Forestry University, Harbin 150040, PR China.
Yu B
Key Laboratory of Plant Ecology, Northeast Forestry University, Harbin 150040, PR China.
Tang Z
Key Laboratory of Plant Ecology, Northeast Forestry University, Harbin 150040, PR China. Electronic address: tangzh@nefu.edu.cn.
Guo Q
Center for Ecological Research, Northeast Forestry University, Harbin 150040, PR China. Electronic address: gqx@nefu.edu.cn.

MeSH

AlkaloidsBiodegradation, EnvironmentalCadmiumCalciumCatharanthusCopperDrug InteractionsMetallothioneinOxidative StressPlant RootsPlants, MedicinalSoilSoil PollutantsZinc

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29626640

Citation

Chen, Qi, et al. "Differential Responses to Cd Stress Induced By Exogenous Application of Cu, Zn or Ca in the Medicinal Plant Catharanthus Roseus." Ecotoxicology and Environmental Safety, vol. 157, 2018, pp. 266-275.
Chen Q, Lu X, Guo X, et al. Differential responses to Cd stress induced by exogenous application of Cu, Zn or Ca in the medicinal plant Catharanthus roseus. Ecotoxicol Environ Saf. 2018;157:266-275.
Chen, Q., Lu, X., Guo, X., Pan, Y., Yu, B., Tang, Z., & Guo, Q. (2018). Differential responses to Cd stress induced by exogenous application of Cu, Zn or Ca in the medicinal plant Catharanthus roseus. Ecotoxicology and Environmental Safety, 157, 266-275. https://doi.org/10.1016/j.ecoenv.2018.03.055
Chen Q, et al. Differential Responses to Cd Stress Induced By Exogenous Application of Cu, Zn or Ca in the Medicinal Plant Catharanthus Roseus. Ecotoxicol Environ Saf. 2018 Aug 15;157:266-275. PubMed PMID: 29626640.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential responses to Cd stress induced by exogenous application of Cu, Zn or Ca in the medicinal plant Catharanthus roseus. AU - Chen,Qi, AU - Lu,Xueyan, AU - Guo,Xiaorui, AU - Pan,Yajie, AU - Yu,Bofan, AU - Tang,Zhonghua, AU - Guo,Qingxi, Y1 - 2018/04/05/ PY - 2017/12/22/received PY - 2018/03/12/revised PY - 2018/03/23/accepted PY - 2018/4/8/pubmed PY - 2018/8/9/medline PY - 2018/4/8/entrez KW - Cadmium toxicity KW - Catharanthus roseus KW - TIA pathway gene KW - Terpenoid indole alkaloid SP - 266 EP - 275 JF - Ecotoxicology and environmental safety JO - Ecotoxicol Environ Saf VL - 157 N2 - Cd(II) is one of the most widespread and toxic heavy metals and seriously threatens plant growth, furthermore negatively affecting human health. For survival from this metal stress, plants always fight with Cd(II) toxicity by themselves or using other external factors. The effects of second metals copper (Cu(II)), zinc (Zn(II)) and calcium (Ca(II)) on the Cd(II)-affected root morphology, Cd(II) translocation and metabolic responses in Catharanthus roseus were investigated under hydroponic conditions. We found that the Cd-stressed plants displayed the browning and rot root symptom, excess H2O2 content, lipid peroxidation and Cd(II) accumulation in plants. However, the supplement with second metals largely alleviated Cd-induced toxicity, including browning and rot roots, oxidative stress and internal Cd(II) accumulation. The amended effects at metabolic and transcriptional levels involved in different second metals share either common or divergent strategies. They commonly repressed Cd uptake and promoted Cd(II) translocation from roots to shoots with divergent mechanisms. High Zn(II) could activate MTs expression in roots, while Cu(II) or Ca(II) did not under Cd(II) stress condition. The presence of Ca(II) under Cd stress condition largely initiated occurrence of lateral roots. We then grouped a metabolic diagram integrating terpenoid indole alkaloid (TIA) accumulation and TIA pathway gene expression to elucidate the metabolic response of C. roseus to Cd(II) alone or combined with second metals. The treatment with 100 Cd(II) alone largely promoted accumulation of vinblastine, vindoline, catharanthine and loganin, whereas depressed or little changed the expression levels of genes detected here, compared to 0 Cd(II) control. In the presence of Cd(II), the supplement with second metals displayed specific effect on different alkaloid. Among them, the metal Ca(II) is especially beneficial for serpentine accumulation, Zn(II) mainly promoted tabersonine production. However, the addition of Cu(II) commonly depressed accumulation of most alkaloids detected here. Generally, we presented different mechanisms by which the second metals used to alleviate Cd (II) toxicity. This plant has potential application in phytoremediation of Cd(II), due to relatively substantial accumulation of biomass, as well as secondary metabolites TIAs used as pharmaceutical materials when facing Cd stress. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/29626640/Differential_responses_to_Cd_stress_induced_by_exogenous_application_of_Cu_Zn_or_Ca_in_the_medicinal_plant_Catharanthus_roseus_ DB - PRIME DP - Unbound Medicine ER -