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Shifts in the relationship between mRNA and protein abundance of gill ion-transporters during smolt development and seawater acclimation in Atlantic salmon (Salmo salar).

Abstract

Smolting Atlantic salmon exhibit a seasonal increase in seawater tolerance that is associated with changes in the abundance of major gill ion-transporter transcripts and proteins. In the present study, we investigate how the transcript and protein abundance of specific ion-transporter isoforms relate to each other during smolt development and seawater acclimation, and how each correlates to seawater tolerance. We show that during smolt development both mRNA and protein abundance of gill Na+/K+-ATPase α1a subunit (NKAα1a) decreased but the decrease in the mRNA was five-times greater than that of the protein. Gill NKAα1b mRNA levels increased only slightly (1.5-fold) throughout development whereas protein abundance increased 30-fold at its peak. Gill Na+/K+/2Cl- co-transporter 1 (NKCC1) increased at the mRNA and protein level (5- and 12-fold) in smolts. The abundance of a gill ion-transporter's mRNA and protein changed in the same direction through development and after seawater transfer, but the changes were not always strongly correlated: NKAα1a (r = 0.768), NKAα1b (r = 0.40), and NKCC1 (r = 0.898). The maintenance of plasma chloride concentration correlated most strongly with the abundance of NKAα1a mRNA, and the ratio of NKAα1b to NKAα1a mRNA and protein. Growth performance after seawater transfer correlated most strongly with the abundance of NKAα1b protein and the ratio of NKAα1b to NKAα1a protein. Our results indicate that the abundance of ion-transporter mRNA and protein do not always correlate well and a decrease in the abundance of gill NKAα1a mRNA and increase in NKAα1b protein are strong predictors of seawater tolerance and growth performance after seawater transfer.

Authors+Show Affiliations

Department of Biology, University of Massachusetts Amherst, Amherst, MA 01003, USA; U.S. Geological Survey, Leetown Science Center, S. O. Conte Anadromous Fish Research Laboratory, Turners Falls, MA 01376, USA. Electronic address: achristensen@annamaria.edu.U.S. Geological Survey, Leetown Science Center, S. O. Conte Anadromous Fish Research Laboratory, Turners Falls, MA 01376, USA.Department of Biology, University of Massachusetts Amherst, Amherst, MA 01003, USA; U.S. Geological Survey, Leetown Science Center, S. O. Conte Anadromous Fish Research Laboratory, Turners Falls, MA 01376, USA.

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

29627325

Citation

Christensen, Arne K., et al. "Shifts in the Relationship Between mRNA and Protein Abundance of Gill Ion-transporters During Smolt Development and Seawater Acclimation in Atlantic Salmon (Salmo Salar)." Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, vol. 221, 2018, pp. 63-73.
Christensen AK, Regish AM, McCormick SD. Shifts in the relationship between mRNA and protein abundance of gill ion-transporters during smolt development and seawater acclimation in Atlantic salmon (Salmo salar). Comp Biochem Physiol, Part A Mol Integr Physiol. 2018;221:63-73.
Christensen, A. K., Regish, A. M., & McCormick, S. D. (2018). Shifts in the relationship between mRNA and protein abundance of gill ion-transporters during smolt development and seawater acclimation in Atlantic salmon (Salmo salar). Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, 221, pp. 63-73. doi:10.1016/j.cbpa.2018.03.020.
Christensen AK, Regish AM, McCormick SD. Shifts in the Relationship Between mRNA and Protein Abundance of Gill Ion-transporters During Smolt Development and Seawater Acclimation in Atlantic Salmon (Salmo Salar). Comp Biochem Physiol, Part A Mol Integr Physiol. 2018;221:63-73. PubMed PMID: 29627325.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Shifts in the relationship between mRNA and protein abundance of gill ion-transporters during smolt development and seawater acclimation in Atlantic salmon (Salmo salar). AU - Christensen,Arne K, AU - Regish,Amy M, AU - McCormick,Stephen D, Y1 - 2018/04/06/ PY - 2018/01/02/received PY - 2018/03/26/revised PY - 2018/03/28/accepted PY - 2018/4/9/pubmed PY - 2019/3/16/medline PY - 2018/4/9/entrez KW - Atlantic salmon KW - Growth KW - Ion-transporters KW - NKA KW - NKCC1 KW - Osmoregulation KW - Salmo salar SP - 63 EP - 73 JF - Comparative biochemistry and physiology. Part A, Molecular & integrative physiology JO - Comp. Biochem. Physiol., Part A Mol. Integr. Physiol. VL - 221 N2 - Smolting Atlantic salmon exhibit a seasonal increase in seawater tolerance that is associated with changes in the abundance of major gill ion-transporter transcripts and proteins. In the present study, we investigate how the transcript and protein abundance of specific ion-transporter isoforms relate to each other during smolt development and seawater acclimation, and how each correlates to seawater tolerance. We show that during smolt development both mRNA and protein abundance of gill Na+/K+-ATPase α1a subunit (NKAα1a) decreased but the decrease in the mRNA was five-times greater than that of the protein. Gill NKAα1b mRNA levels increased only slightly (1.5-fold) throughout development whereas protein abundance increased 30-fold at its peak. Gill Na+/K+/2Cl- co-transporter 1 (NKCC1) increased at the mRNA and protein level (5- and 12-fold) in smolts. The abundance of a gill ion-transporter's mRNA and protein changed in the same direction through development and after seawater transfer, but the changes were not always strongly correlated: NKAα1a (r = 0.768), NKAα1b (r = 0.40), and NKCC1 (r = 0.898). The maintenance of plasma chloride concentration correlated most strongly with the abundance of NKAα1a mRNA, and the ratio of NKAα1b to NKAα1a mRNA and protein. Growth performance after seawater transfer correlated most strongly with the abundance of NKAα1b protein and the ratio of NKAα1b to NKAα1a protein. Our results indicate that the abundance of ion-transporter mRNA and protein do not always correlate well and a decrease in the abundance of gill NKAα1a mRNA and increase in NKAα1b protein are strong predictors of seawater tolerance and growth performance after seawater transfer. SN - 1531-4332 UR - https://www.unboundmedicine.com/medline/citation/29627325/Shifts_in_the_relationship_between_mRNA_and_protein_abundance_of_gill_ion_transporters_during_smolt_development_and_seawater_acclimation_in_Atlantic_salmon__Salmo_salar__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1095-6433(18)30050-3 DB - PRIME DP - Unbound Medicine ER -