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Phytochemical characterization of polyphenolic compounds with HPLC-DAD-ESI-MS and evaluation of lipid-lowering capacity of aqueous extracts from Saharan plant Anabasis aretioides (Coss & Moq.) in normal and streptozotocin-induced diabetic rats.
J Integr Med. 2018 05; 16(3):185-191.JI

Abstract

OBJECTIVE

Anabasis aretioides (Coss & Moq.), a Saharan plant belonging to Chenopodiaceae family, is widely distributed in semi-desert areas from the Tafilalet region of Morocco. This plant is extensively used by local population against diabetes and cardiovascular disorders. The purpose of the study was to investigate the effect of the aqueous A. aretioides extract on lipid metabolism in normal and streptozotocin (STZ)-induced diabetic rats and to identify the polyphenolic compounds present. In addition, the in vitro antioxidant activity of the aqueous A. aretioides extract was also evaluated.

METHODS

The effect of an aerial part aqueous extract (APAE) of A. aretioides (5 mg/kg of lyophilized A. aretioides APAE) on plasma lipid profile was investigated in normal and STZ-induced diabetic rats (n = 6) after once daily oral administration for 15 days. The aqueous extract was tested for its 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity. Polyphenolic compounds in the extracts were definitively characterized by high-performance liquid chromatography-diode array detection-electrospray ionization-mass spectrometry.

RESULTS

In diabetic rats, oral administration of A. aretioides APAE provoked a significant decrease in both plasma cholesterol and triglyceride levels from the first to the second week (P < 0.01). A significant decrease on plasma triglyceride levels was also observed in normal rats (P < 0.01), where the reduction was 53%. In addition, the phytochemical analysis revealed the presence of 12 polyphenolic compounds. Moreover, according to the DPPH radical-scavenging activity, the aqueous extract showed an in vitro antioxidant activity.

CONCLUSION

Aqueous A. aretioides APAE exhibits lipid-lowering and in vitro antioxidant activities. Many polyphenols were present in this extract and these phytoconstituents may be involved in the pharmacological activity of this plant.

Authors+Show Affiliations

Team of Physiology & Endocrine Pharmacology, Faculty of Sciences and Techniques Errachidia, Moulay Ismail University, BP 509, Boutalamine, Errachidia 52000, Morocco.Team of Physiology & Endocrine Pharmacology, Faculty of Sciences and Techniques Errachidia, Moulay Ismail University, BP 509, Boutalamine, Errachidia 52000, Morocco; Division of Preventive Oncology, National Center for Tumor Diseases, Im Neuenheimer Feld 460, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 581, Heidelberg, Germany.Team of Physiology & Endocrine Pharmacology, Faculty of Sciences and Techniques Errachidia, Moulay Ismail University, BP 509, Boutalamine, Errachidia 52000, Morocco.Division of Preventive Oncology, National Center for Tumor Diseases, Im Neuenheimer Feld 460, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 581, Heidelberg, Germany.Division of Preventive Oncology, National Center for Tumor Diseases, Im Neuenheimer Feld 460, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 581, Heidelberg, Germany.Team of Physiology & Endocrine Pharmacology, Faculty of Sciences and Techniques Errachidia, Moulay Ismail University, BP 509, Boutalamine, Errachidia 52000, Morocco. Electronic address: mohamed.eddouks@laposte.net.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29631911

Citation

Farid, Omar, et al. "Phytochemical Characterization of Polyphenolic Compounds With HPLC-DAD-ESI-MS and Evaluation of Lipid-lowering Capacity of Aqueous Extracts From Saharan Plant Anabasis Aretioides (Coss & Moq.) in Normal and Streptozotocin-induced Diabetic Rats." Journal of Integrative Medicine, vol. 16, no. 3, 2018, pp. 185-191.
Farid O, Khallouki F, Akdad M, et al. Phytochemical characterization of polyphenolic compounds with HPLC-DAD-ESI-MS and evaluation of lipid-lowering capacity of aqueous extracts from Saharan plant Anabasis aretioides (Coss & Moq.) in normal and streptozotocin-induced diabetic rats. J Integr Med. 2018;16(3):185-191.
Farid, O., Khallouki, F., Akdad, M., Breuer, A., Owen, R. W., & Eddouks, M. (2018). Phytochemical characterization of polyphenolic compounds with HPLC-DAD-ESI-MS and evaluation of lipid-lowering capacity of aqueous extracts from Saharan plant Anabasis aretioides (Coss & Moq.) in normal and streptozotocin-induced diabetic rats. Journal of Integrative Medicine, 16(3), 185-191. https://doi.org/10.1016/j.joim.2018.03.003
Farid O, et al. Phytochemical Characterization of Polyphenolic Compounds With HPLC-DAD-ESI-MS and Evaluation of Lipid-lowering Capacity of Aqueous Extracts From Saharan Plant Anabasis Aretioides (Coss & Moq.) in Normal and Streptozotocin-induced Diabetic Rats. J Integr Med. 2018;16(3):185-191. PubMed PMID: 29631911.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phytochemical characterization of polyphenolic compounds with HPLC-DAD-ESI-MS and evaluation of lipid-lowering capacity of aqueous extracts from Saharan plant Anabasis aretioides (Coss & Moq.) in normal and streptozotocin-induced diabetic rats. AU - Farid,Omar, AU - Khallouki,Farid, AU - Akdad,Morad, AU - Breuer,Andrea, AU - Owen,Robert Wyn, AU - Eddouks,Mohamed, Y1 - 2018/03/28/ PY - 2017/07/26/received PY - 2017/12/11/accepted PY - 2018/4/11/pubmed PY - 2018/10/27/medline PY - 2018/4/11/entrez KW - 1,1-Diphenyl-2-picrylhydrazyl KW - Anabasis aretioides KW - Antioxidant activity KW - Lipid metabolism KW - Phytochemical analysis KW - Polyphenolic compounds SP - 185 EP - 191 JF - Journal of integrative medicine JO - J Integr Med VL - 16 IS - 3 N2 - OBJECTIVE: Anabasis aretioides (Coss & Moq.), a Saharan plant belonging to Chenopodiaceae family, is widely distributed in semi-desert areas from the Tafilalet region of Morocco. This plant is extensively used by local population against diabetes and cardiovascular disorders. The purpose of the study was to investigate the effect of the aqueous A. aretioides extract on lipid metabolism in normal and streptozotocin (STZ)-induced diabetic rats and to identify the polyphenolic compounds present. In addition, the in vitro antioxidant activity of the aqueous A. aretioides extract was also evaluated. METHODS: The effect of an aerial part aqueous extract (APAE) of A. aretioides (5 mg/kg of lyophilized A. aretioides APAE) on plasma lipid profile was investigated in normal and STZ-induced diabetic rats (n = 6) after once daily oral administration for 15 days. The aqueous extract was tested for its 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity. Polyphenolic compounds in the extracts were definitively characterized by high-performance liquid chromatography-diode array detection-electrospray ionization-mass spectrometry. RESULTS: In diabetic rats, oral administration of A. aretioides APAE provoked a significant decrease in both plasma cholesterol and triglyceride levels from the first to the second week (P < 0.01). A significant decrease on plasma triglyceride levels was also observed in normal rats (P < 0.01), where the reduction was 53%. In addition, the phytochemical analysis revealed the presence of 12 polyphenolic compounds. Moreover, according to the DPPH radical-scavenging activity, the aqueous extract showed an in vitro antioxidant activity. CONCLUSION: Aqueous A. aretioides APAE exhibits lipid-lowering and in vitro antioxidant activities. Many polyphenols were present in this extract and these phytoconstituents may be involved in the pharmacological activity of this plant. SN - 2095-4964 UR - https://www.unboundmedicine.com/medline/citation/29631911/Phytochemical_characterization_of_polyphenolic_compounds_with_HPLC_DAD_ESI_MS_and_evaluation_of_lipid_lowering_capacity_of_aqueous_extracts_from_Saharan_plant_Anabasis_aretioides__Coss_&_Moq___in_normal_and_streptozotocin_induced_diabetic_rats_ DB - PRIME DP - Unbound Medicine ER -