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Proliferation of prostate epithelia induced by IL-6 from stroma reacted with Trichomonas vaginalis.
Parasite Immunol 2018; 40(6):e12531PI

Abstract

Benign prostatic hyperplasia (BPH) is characterized by the proliferation of stromal and epithelial cell types in the prostate, and interactions between the two types of cells. We demonstrated previously that proliferation of prostate stromal cells was induced by BPH epithelial cells in response to Trichomonas vaginalis (Tv) infection via crosstalk with mast cells. In this study, we investigated whether IL-6 released by the proliferating stromal cells in turn induce the BPH epithelial cells to multiply. When culture supernatants of the proliferating prostate stromal cells were added to BPH epithelial cells, the latter multiplied, and expression of cyclin D1, FGF2 and Bcl-2 increased. Blocking the IL-6 signalling pathway with anti-IL-6R antibody or JAK1/2 inhibitor inhibited the proliferation of the BPH epithelial cells and reduced the expression of IL-6, IL-6R and STAT3. Also, epithelial-mesenchymal transition was detected in the proliferating BPH epithelial cells. In conclusion, IL-6 released from proliferating prostate stromal cells induced by BPH epithelial cells infected with Tv in turn induces multiplication of the BPH epithelial cells. This result provides first evidence that the inflammatory microenvironment of prostate stromal cells resulting from Tv infection induces the proliferation of prostate epithelial cells by stromal-epithelial interaction.

Authors+Show Affiliations

Department of Environmental Biology and Medical Parasitology, Hanyang University College of Medicine, Seoul, Korea. Department of Biomedical Science, Graduate School of Biomedical Science & Engineering, Seoul, Korea.Department of Environmental Biology and Medical Parasitology, Hanyang University College of Medicine, Seoul, Korea.Department of Biochemistry and Molecular Biology, Hanyang University College of Medicine, Seoul, Korea.Department of Internal Medicine, Hallym University Han River Seongshim Hospital, Seoul, Korea.Department of Environmental Biology and Medical Parasitology, Hanyang University College of Medicine, Seoul, Korea.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29633291

Citation

Kim, J-H, et al. "Proliferation of Prostate Epithelia Induced By IL-6 From Stroma Reacted With Trichomonas Vaginalis." Parasite Immunology, vol. 40, no. 6, 2018, pp. e12531.
Kim JH, Han IH, Kim YS, et al. Proliferation of prostate epithelia induced by IL-6 from stroma reacted with Trichomonas vaginalis. Parasite Immunol. 2018;40(6):e12531.
Kim, J. H., Han, I. H., Kim, Y. S., Noh, C. S., & Ryu, J. S. (2018). Proliferation of prostate epithelia induced by IL-6 from stroma reacted with Trichomonas vaginalis. Parasite Immunology, 40(6), pp. e12531. doi:10.1111/pim.12531.
Kim JH, et al. Proliferation of Prostate Epithelia Induced By IL-6 From Stroma Reacted With Trichomonas Vaginalis. Parasite Immunol. 2018;40(6):e12531. PubMed PMID: 29633291.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proliferation of prostate epithelia induced by IL-6 from stroma reacted with Trichomonas vaginalis. AU - Kim,J-H, AU - Han,I-H, AU - Kim,Y-S, AU - Noh,C-S, AU - Ryu,J-S, Y1 - 2018/04/26/ PY - 2018/01/03/received PY - 2018/03/27/accepted PY - 2018/4/11/pubmed PY - 2019/4/17/medline PY - 2018/4/11/entrez KW - cytokines KW - inflammation KW - prostatic hyperplasia KW - protozoan infections SP - e12531 EP - e12531 JF - Parasite immunology JO - Parasite Immunol. VL - 40 IS - 6 N2 - Benign prostatic hyperplasia (BPH) is characterized by the proliferation of stromal and epithelial cell types in the prostate, and interactions between the two types of cells. We demonstrated previously that proliferation of prostate stromal cells was induced by BPH epithelial cells in response to Trichomonas vaginalis (Tv) infection via crosstalk with mast cells. In this study, we investigated whether IL-6 released by the proliferating stromal cells in turn induce the BPH epithelial cells to multiply. When culture supernatants of the proliferating prostate stromal cells were added to BPH epithelial cells, the latter multiplied, and expression of cyclin D1, FGF2 and Bcl-2 increased. Blocking the IL-6 signalling pathway with anti-IL-6R antibody or JAK1/2 inhibitor inhibited the proliferation of the BPH epithelial cells and reduced the expression of IL-6, IL-6R and STAT3. Also, epithelial-mesenchymal transition was detected in the proliferating BPH epithelial cells. In conclusion, IL-6 released from proliferating prostate stromal cells induced by BPH epithelial cells infected with Tv in turn induces multiplication of the BPH epithelial cells. This result provides first evidence that the inflammatory microenvironment of prostate stromal cells resulting from Tv infection induces the proliferation of prostate epithelial cells by stromal-epithelial interaction. SN - 1365-3024 UR - https://www.unboundmedicine.com/medline/citation/29633291/Proliferation_of_prostate_epithelia_induced_by_IL_6_from_stroma_reacted_with_Trichomonas_vaginalis_ L2 - https://doi.org/10.1111/pim.12531 DB - PRIME DP - Unbound Medicine ER -