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Posttreatment Effects of Olea Europaea L. Leaf Extract on Carbon Tetrachloride-Induced Liver Injury and Oxidative Stress in Rats.
J Med Food. 2018 Sep; 21(9):899-904.JM

Abstract

The aim of this study is to examine the therapeutic effects of Olea europaea L. leaf extract on carbon tetrachloride (CCl4)-induced liver damage in rats. In the experiments, 3- to 4-month-old 28 male Sprague-Dawley rats were divided into four groups: control, O. europaea leaf extract, CCl4, and curative. The CCl4 and curative groups received CCl4 (0.2 mL/kg) intraperitoneally for 10 days to form hepatic injury. O. europaea (80 mg/kg) leaf extract was given orally to the curative group dissolved in distilled water the following 14 days. Hepatic and antioxidant enzyme levels, p53, caspase 3, lipid peroxidation marker malondialdehyde (MDA), and also DNA fragmentation levels were determined to establish oxidative stress in hepatic cell damage and its consequences. After formation of liver damage, oral administration of the O. europaea significantly reduced CCl4-induced elevations of serum alkaline phosphatase, aspartate aminotransferase and alanine aminotransferase levels (P < .001), MDA levels of both blood (P < .001) and liver tissues (P < .001), DNA fragmentation (P < .001), p53 (P < .001), and caspase 3 (P < .001) levels of liver tissues. Also this administration in curative group significantly increased CCl4-induced reductions of superoxide dismutase (SOD) (P < .001) and catalase (CAT) (P < .001) activity of blood samples and decreased SOD (P < .001) and CAT (P < .05) activity observed in liver tissue curative groups compared with CCl4 curative group. In CCl4 group, liver tissue samples exhibited remarkable damage because of CCl4 and reduction of these damages were observed in the curative group. Our results showed that O. europaea leaf extract was effective in reducing hepatic damage caused by CCl4 by reducing lipid peroxidation, regulating antioxidant enzymes, and minimizing DNA damage.

Authors+Show Affiliations

1 Department of Medical Laboratory, Vocational School of Health Services, Eskisehir Osmangazi University , Eskisehir, Turkey .2 Department of Medical Biology, Eskisehir Osmangazi University , Eskisehir, Turkey .3 Department of Medical Oncology, Faculty of Medicine, Eskisehir Osmangazi University , Eskisehir, Turkey .4 Department of Biotechnology and Molecular Biology, Aksaray University , Aksaray, Turkey .5 Department of Histology and Embryology, Faculty of Medicine, Eskisehir Osmangazi University , Eskisehir, Turkey .6 Department of Medical Biochemistry, Faculty of Medicine, Eskisehir Osmangazi University , Eskisehir, Turkey .7 Department of Biostatistics and Medical Informatics, Faculty of Medicine, Eskisehir Osmangazi University , Eskisehir, Turkey .2 Department of Medical Biology, Eskisehir Osmangazi University , Eskisehir, Turkey .8 Department of Nuclear Medicine, Faculty of Medicine, Eskisehir Osmangazi University , Eskisehir, Turkey .2 Department of Medical Biology, Eskisehir Osmangazi University , Eskisehir, Turkey .

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29648970

Citation

Ustuner, Derya, et al. "Posttreatment Effects of Olea Europaea L. Leaf Extract On Carbon Tetrachloride-Induced Liver Injury and Oxidative Stress in Rats." Journal of Medicinal Food, vol. 21, no. 9, 2018, pp. 899-904.
Ustuner D, Colak E, Dincer M, et al. Posttreatment Effects of Olea Europaea L. Leaf Extract on Carbon Tetrachloride-Induced Liver Injury and Oxidative Stress in Rats. J Med Food. 2018;21(9):899-904.
Ustuner, D., Colak, E., Dincer, M., Tekin, N., Burukoglu Donmez, D., Akyuz, F., Colak, E., Kolaç, U. K., Entok, E., & Ustuner, M. C. (2018). Posttreatment Effects of Olea Europaea L. Leaf Extract on Carbon Tetrachloride-Induced Liver Injury and Oxidative Stress in Rats. Journal of Medicinal Food, 21(9), 899-904. https://doi.org/10.1089/jmf.2017.0143
Ustuner D, et al. Posttreatment Effects of Olea Europaea L. Leaf Extract On Carbon Tetrachloride-Induced Liver Injury and Oxidative Stress in Rats. J Med Food. 2018;21(9):899-904. PubMed PMID: 29648970.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Posttreatment Effects of Olea Europaea L. Leaf Extract on Carbon Tetrachloride-Induced Liver Injury and Oxidative Stress in Rats. AU - Ustuner,Derya, AU - Colak,Emine, AU - Dincer,Murat, AU - Tekin,Neslihan, AU - Burukoglu Donmez,Dilek, AU - Akyuz,Fahrettin, AU - Colak,Ertugrul, AU - Kolaç,Umut Kerem, AU - Entok,Emre, AU - Ustuner,Mehmet Cengiz, Y1 - 2018/03/20/ PY - 2018/4/13/pubmed PY - 2018/12/15/medline PY - 2018/4/13/entrez KW - DNA fragmentation KW - antioxidants KW - liver damage KW - p53 SP - 899 EP - 904 JF - Journal of medicinal food JO - J Med Food VL - 21 IS - 9 N2 - The aim of this study is to examine the therapeutic effects of Olea europaea L. leaf extract on carbon tetrachloride (CCl4)-induced liver damage in rats. In the experiments, 3- to 4-month-old 28 male Sprague-Dawley rats were divided into four groups: control, O. europaea leaf extract, CCl4, and curative. The CCl4 and curative groups received CCl4 (0.2 mL/kg) intraperitoneally for 10 days to form hepatic injury. O. europaea (80 mg/kg) leaf extract was given orally to the curative group dissolved in distilled water the following 14 days. Hepatic and antioxidant enzyme levels, p53, caspase 3, lipid peroxidation marker malondialdehyde (MDA), and also DNA fragmentation levels were determined to establish oxidative stress in hepatic cell damage and its consequences. After formation of liver damage, oral administration of the O. europaea significantly reduced CCl4-induced elevations of serum alkaline phosphatase, aspartate aminotransferase and alanine aminotransferase levels (P < .001), MDA levels of both blood (P < .001) and liver tissues (P < .001), DNA fragmentation (P < .001), p53 (P < .001), and caspase 3 (P < .001) levels of liver tissues. Also this administration in curative group significantly increased CCl4-induced reductions of superoxide dismutase (SOD) (P < .001) and catalase (CAT) (P < .001) activity of blood samples and decreased SOD (P < .001) and CAT (P < .05) activity observed in liver tissue curative groups compared with CCl4 curative group. In CCl4 group, liver tissue samples exhibited remarkable damage because of CCl4 and reduction of these damages were observed in the curative group. Our results showed that O. europaea leaf extract was effective in reducing hepatic damage caused by CCl4 by reducing lipid peroxidation, regulating antioxidant enzymes, and minimizing DNA damage. SN - 1557-7600 UR - https://www.unboundmedicine.com/medline/citation/29648970/Posttreatment_Effects_of_Olea_Europaea_L__Leaf_Extract_on_Carbon_Tetrachloride_Induced_Liver_Injury_and_Oxidative_Stress_in_Rats_ L2 - https://www.liebertpub.com/doi/full/10.1089/jmf.2017.0143?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -