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In-field and in-vitro study of the moss Leptodictyum riparium as bioindicator of toxic metal pollution in the aquatic environment: Ultrastructural damage, oxidative stress and HSP70 induction.
PLoS One. 2018; 13(4):e0195717.Plos

Abstract

This study evaluates the effects of toxic metal pollution in the highly contaminated Sarno River (South Italy), by using the aquatic moss Leptodictyum riparium in bags at 3 representative sites of the river. Biological effects were assessed by metal bioaccumulation, ultrastructural changes, oxidative stress, as Reactive Oxygen Species (ROS) production and Glutathione S-transferase (GST) activity, as well as Heat Shock Proteins 70 (HSP70s) induction. The results showed that L. riparium is a valuable bioindicator for toxic metal pollution of water ecosystem, accumulating different amounts of toxic metals from the aquatic environment. Toxic metal pollution caused severe ultrastructural damage, as well as increased ROS production and induction of GST and HSP70s, in the samples exposed at the polluted sites. To assess the role and the effect of toxic metals on L. riparium, were also cultured in vitro with Cd, Cr, Cu, Fe, Ni, Pb, Zn at the same concentrations as measured at the 3 sites. Ultrastructure, ROS, GST, and HSP70s resulted severely affected by toxic metals. Based on our findings, we confirm L. riparium as a model organism in freshwater biomonitoring surveys, and GST and HSP70s as promising biomarkers of metal toxicity.

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Authors+Show Affiliations

Esposito S
Dipartimento di Biologia, University of Naples Federico II, Complesso Univ. Monte Sant'Angelo, Napoli, Italy.
Loppi S
Dipartimento di Scienze della Vita, University of Siena, Siena, Italy.
Monaci F
Dipartimento di Scienze della Vita, University of Siena, Siena, Italy.
Paoli L
Dipartimento di Scienze della Vita, University of Siena, Siena, Italy.
Vannini A
Dipartimento di Scienze della Vita, University of Siena, Siena, Italy.
Sorbo S
Ce.S.M.A, Section of Microscopy, University of Naples Federico II, Complesso Univ. Monte Sant'Angelo, Napoli, Italy.
Maresca V
Dipartimento di Biologia, University of Naples Federico II, Complesso Univ. Monte Sant'Angelo, Napoli, Italy.
Fusaro L
Dipartimento di Biologia Ambientale, Università Sapienza, Roma, Italy.
Asadi Karam E
Biology Department, Shahid Bahonar University of Kerman, Kerman, Iran.
Lentini M
Dipartimento di Biologia, University of Naples Federico II, Complesso Univ. Monte Sant'Angelo, Napoli, Italy.
De Lillo A
Dipartimento di Biologia, University of Naples Federico II, Complesso Univ. Monte Sant'Angelo, Napoli, Italy.
Conte B
Dipartimento di Biologia, University of Naples Federico II, Complesso Univ. Monte Sant'Angelo, Napoli, Italy.
Cianciullo P
Dipartimento di Scienze della Vita, University of Siena, Siena, Italy.
Basile A
Dipartimento di Biologia, University of Naples Federico II, Complesso Univ. Monte Sant'Angelo, Napoli, Italy.

MeSH

BryopsidaGlutathione TransferaseHSP70 Heat-Shock ProteinsItalyMetals, HeavyMicroscopy, Electron, TransmissionOxidative StressPlant ProteinsRiversSentinel SpeciesWater Pollutants, ChemicalWater Pollution, ChemicalWater Quality

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29649272

Citation

Esposito, Sergio, et al. "In-field and In-vitro Study of the Moss Leptodictyum Riparium as Bioindicator of Toxic Metal Pollution in the Aquatic Environment: Ultrastructural Damage, Oxidative Stress and HSP70 Induction." PloS One, vol. 13, no. 4, 2018, pp. e0195717.
Esposito S, Loppi S, Monaci F, et al. In-field and in-vitro study of the moss Leptodictyum riparium as bioindicator of toxic metal pollution in the aquatic environment: Ultrastructural damage, oxidative stress and HSP70 induction. PLoS One. 2018;13(4):e0195717.
Esposito, S., Loppi, S., Monaci, F., Paoli, L., Vannini, A., Sorbo, S., Maresca, V., Fusaro, L., Asadi Karam, E., Lentini, M., De Lillo, A., Conte, B., Cianciullo, P., & Basile, A. (2018). In-field and in-vitro study of the moss Leptodictyum riparium as bioindicator of toxic metal pollution in the aquatic environment: Ultrastructural damage, oxidative stress and HSP70 induction. PloS One, 13(4), e0195717. https://doi.org/10.1371/journal.pone.0195717
Esposito S, et al. In-field and In-vitro Study of the Moss Leptodictyum Riparium as Bioindicator of Toxic Metal Pollution in the Aquatic Environment: Ultrastructural Damage, Oxidative Stress and HSP70 Induction. PLoS One. 2018;13(4):e0195717. PubMed PMID: 29649272.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In-field and in-vitro study of the moss Leptodictyum riparium as bioindicator of toxic metal pollution in the aquatic environment: Ultrastructural damage, oxidative stress and HSP70 induction. AU - Esposito,Sergio, AU - Loppi,Stefano, AU - Monaci,Fabrizio, AU - Paoli,Luca, AU - Vannini,Andrea, AU - Sorbo,Sergio, AU - Maresca,Viviana, AU - Fusaro,Lina, AU - Asadi Karam,Elham, AU - Lentini,Marco, AU - De Lillo,Alessia, AU - Conte,Barbara, AU - Cianciullo,Piergiorgio, AU - Basile,Adriana, Y1 - 2018/04/12/ PY - 2017/12/21/received PY - 2018/03/28/accepted PY - 2018/4/13/entrez PY - 2018/4/13/pubmed PY - 2018/7/26/medline SP - e0195717 EP - e0195717 JF - PloS one JO - PLoS One VL - 13 IS - 4 N2 - This study evaluates the effects of toxic metal pollution in the highly contaminated Sarno River (South Italy), by using the aquatic moss Leptodictyum riparium in bags at 3 representative sites of the river. Biological effects were assessed by metal bioaccumulation, ultrastructural changes, oxidative stress, as Reactive Oxygen Species (ROS) production and Glutathione S-transferase (GST) activity, as well as Heat Shock Proteins 70 (HSP70s) induction. The results showed that L. riparium is a valuable bioindicator for toxic metal pollution of water ecosystem, accumulating different amounts of toxic metals from the aquatic environment. Toxic metal pollution caused severe ultrastructural damage, as well as increased ROS production and induction of GST and HSP70s, in the samples exposed at the polluted sites. To assess the role and the effect of toxic metals on L. riparium, were also cultured in vitro with Cd, Cr, Cu, Fe, Ni, Pb, Zn at the same concentrations as measured at the 3 sites. Ultrastructure, ROS, GST, and HSP70s resulted severely affected by toxic metals. Based on our findings, we confirm L. riparium as a model organism in freshwater biomonitoring surveys, and GST and HSP70s as promising biomarkers of metal toxicity. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/29649272/In_field_and_in_vitro_study_of_the_moss_Leptodictyum_riparium_as_bioindicator_of_toxic_metal_pollution_in_the_aquatic_environment:_Ultrastructural_damage_oxidative_stress_and_HSP70_induction_ DB - PRIME DP - Unbound Medicine ER -