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Roles of progesterone receptor membrane component 1 and membrane progestin receptor alpha in regulation of zebrafish oocyte maturation.
Gen Comp Endocrinol 2018; 263:51-61GC

Abstract

Although previous studies suggest membrane progesterone receptor alpha (mPRα/Paqr7) mediates 17, 20β-dihydroxy-4-pregnen-3-one (DHP) induction of oocyte maturation (OM) in zebrafish, critical information needed to establish mPRα as the receptor mediating OM is lacking. The relative potencies of progestins and specific mPRα agonists in inducing OM matched their relative binding affinities for zebrafish mPRα, supporting its role in OM. Microinjection of pertussis toxin blocked DHP induction of OM and the progestin-induced decrease in cyclic AMP levels, suggesting mPRα activates an inhibitory G protein (Gi). Microinjection of morpholino antisense oligonucleotides to zebrafish pgrmc1 blocked induction of OM by DHP which was accompanied by decreased levels of Pgrmc1 and mPRα on the oocyte plasma membranes. Similarly, treatment of denuded oocytes with a PGRMC1 inhibitor, AG205, blocked the gonadotropin-induced increase in plasma membrane mPRα levels and attenuated DHP induction of OM. Co-incubation with two inhibitors of epidermal growth factor Erbb2, ErbB2 inhibitor II and AG 879, prevented induction of OM by DHP, indicating the likely involvement of Erbb2 in mPRα-mediated signaling. Treatment with AG205 reversed the inhibitory effects of the Erbb2 inhibitors on OM and also inhibited insulin-like growth factor-1 induction of OM. Close associations between Pgrmc1 and mPRα, and between Pgrmc1 and Erbb2 were detected in zebrafish oocytes with in situ proximity ligation assays. The results suggest progestin induction of OM in zebrafish is mediated through an mPRα/Gi/Erbb2 signaling pathway that requires Pgrmc1 for expression of mPRα on oocyte membranes and that Pgrmc1 also is required for induction of OM through Erbb2.

Authors+Show Affiliations

The University of Texas at Austin, Marine Science Institute, 750 Channel View Drive, Port Aransas, TX 78373, USA.The University of Texas at Austin, Marine Science Institute, 750 Channel View Drive, Port Aransas, TX 78373, USA.The University of Texas at Austin, Marine Science Institute, 750 Channel View Drive, Port Aransas, TX 78373, USA.The University of Texas at Austin, Marine Science Institute, 750 Channel View Drive, Port Aransas, TX 78373, USA.East Carolina University, Department of Biology, Greenville, NC 27858, USA.The University of Texas at Austin, Marine Science Institute, 750 Channel View Drive, Port Aransas, TX 78373, USA.The University of Texas at Austin, Marine Science Institute, 750 Channel View Drive, Port Aransas, TX 78373, USA. Electronic address: peter.thomas@utexas.edu.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29649418

Citation

Aizen, Joseph, et al. "Roles of Progesterone Receptor Membrane Component 1 and Membrane Progestin Receptor Alpha in Regulation of Zebrafish Oocyte Maturation." General and Comparative Endocrinology, vol. 263, 2018, pp. 51-61.
Aizen J, Pang Y, Harris C, et al. Roles of progesterone receptor membrane component 1 and membrane progestin receptor alpha in regulation of zebrafish oocyte maturation. Gen Comp Endocrinol. 2018;263:51-61.
Aizen, J., Pang, Y., Harris, C., Converse, A., Zhu, Y., Aguirre, M. A., & Thomas, P. (2018). Roles of progesterone receptor membrane component 1 and membrane progestin receptor alpha in regulation of zebrafish oocyte maturation. General and Comparative Endocrinology, 263, pp. 51-61. doi:10.1016/j.ygcen.2018.04.009.
Aizen J, et al. Roles of Progesterone Receptor Membrane Component 1 and Membrane Progestin Receptor Alpha in Regulation of Zebrafish Oocyte Maturation. Gen Comp Endocrinol. 2018 07 1;263:51-61. PubMed PMID: 29649418.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Roles of progesterone receptor membrane component 1 and membrane progestin receptor alpha in regulation of zebrafish oocyte maturation. AU - Aizen,Joseph, AU - Pang,Yefei, AU - Harris,Caleb, AU - Converse,Aubrey, AU - Zhu,Yong, AU - Aguirre,Meagan A, AU - Thomas,Peter, Y1 - 2018/04/09/ PY - 2017/11/03/received PY - 2018/03/06/revised PY - 2018/04/07/accepted PY - 2018/4/13/pubmed PY - 2018/7/24/medline PY - 2018/4/13/entrez KW - AG205 KW - Adaptor protein KW - Erbb2 KW - IGF-1 KW - Oocyte maturation KW - Pgrmc1 KW - Zebrafish KW - mPRα SP - 51 EP - 61 JF - General and comparative endocrinology JO - Gen. Comp. Endocrinol. VL - 263 N2 - Although previous studies suggest membrane progesterone receptor alpha (mPRα/Paqr7) mediates 17, 20β-dihydroxy-4-pregnen-3-one (DHP) induction of oocyte maturation (OM) in zebrafish, critical information needed to establish mPRα as the receptor mediating OM is lacking. The relative potencies of progestins and specific mPRα agonists in inducing OM matched their relative binding affinities for zebrafish mPRα, supporting its role in OM. Microinjection of pertussis toxin blocked DHP induction of OM and the progestin-induced decrease in cyclic AMP levels, suggesting mPRα activates an inhibitory G protein (Gi). Microinjection of morpholino antisense oligonucleotides to zebrafish pgrmc1 blocked induction of OM by DHP which was accompanied by decreased levels of Pgrmc1 and mPRα on the oocyte plasma membranes. Similarly, treatment of denuded oocytes with a PGRMC1 inhibitor, AG205, blocked the gonadotropin-induced increase in plasma membrane mPRα levels and attenuated DHP induction of OM. Co-incubation with two inhibitors of epidermal growth factor Erbb2, ErbB2 inhibitor II and AG 879, prevented induction of OM by DHP, indicating the likely involvement of Erbb2 in mPRα-mediated signaling. Treatment with AG205 reversed the inhibitory effects of the Erbb2 inhibitors on OM and also inhibited insulin-like growth factor-1 induction of OM. Close associations between Pgrmc1 and mPRα, and between Pgrmc1 and Erbb2 were detected in zebrafish oocytes with in situ proximity ligation assays. The results suggest progestin induction of OM in zebrafish is mediated through an mPRα/Gi/Erbb2 signaling pathway that requires Pgrmc1 for expression of mPRα on oocyte membranes and that Pgrmc1 also is required for induction of OM through Erbb2. SN - 1095-6840 UR - https://www.unboundmedicine.com/medline/citation/29649418/Roles_of_progesterone_receptor_membrane_component_1_and_membrane_progestin_receptor_alpha_in_regulation_of_zebrafish_oocyte_maturation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0016-6480(17)30757-8 DB - PRIME DP - Unbound Medicine ER -