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Skeletal muscle stem cell characteristics and myonuclei content in patients with rheumatoid arthritis: a cross-sectional study.
Rheumatol Int. 2018 Jun; 38(6):1031-1041.RI

Abstract

To investigate satellite cells (SCs) and myonuclei characteristics in patients with rheumatoid arthritis (RA). Resting biopsies from m. vastus lateralis were obtained from thirteen RA patients and thirteen matched healthy controls (CON). Muscle biopsies were immunohistochemically stained and analyzed for fiber type specific content of SCs (Pax7+), proliferating SCs (Pax7+/MyoD+) and differentiating SCs (myogenin+). Furthermore, we quantified fiber type specific content of myonuclei and myofiber cross-sectional area (CSA). Finally, newly formed/regenerating fibers expressing neonatal MHC (nMHC+) were determined. The fiber type specific number of SCs did not differ between RA patients and CON, nor did the content of proliferating or differentiating SCs. In contrast, the content of myonuclei per fiber was higher in RA patients than CON for both type I (2.01 ± 0.41 vs. 1.42 ± 0.40 myonuclei/fiber, p < 0.01) and type II fibers (2.01 ± 0.41 vs. 1.37 ± 0.32 myonuclei/fiber, p < 0.01). No differences were observed in fiber composition, fiber type specific CSA or content of nMHC+ fibers. Our results indicate an increased propensity for myogenic differentiation of SC leading to an elevated myonuclear content in the skeletal muscle of RA patients. It is hypothesized that this could be a compensatory regulatory response related to the chronic inflammation in these patients.

Authors+Show Affiliations

Section for Sport Science, Department of Public Health, Aarhus University, Dalgas Avenue 6, 8000, Aarhus C, Denmark. rjb@outlook.dk.Research Laboratory for Biochemical Pathology, Department for Clinical Medicine, Aarhus University, Aarhus, Denmark.Section for Sport Science, Department of Public Health, Aarhus University, Dalgas Avenue 6, 8000, Aarhus C, Denmark.Institute of Sports Medicine, Department of Orthopaedic Surgery M, Bispebjerg Hospital and Center Healthy Aging, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.Section for Sport Science, Department of Public Health, Aarhus University, Dalgas Avenue 6, 8000, Aarhus C, Denmark. Institute of Sports Medicine, Department of Orthopaedic Surgery M, Bispebjerg Hospital and Center Healthy Aging, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29651539

Citation

Boutrup, Rasmus Jentoft, et al. "Skeletal Muscle Stem Cell Characteristics and Myonuclei Content in Patients With Rheumatoid Arthritis: a Cross-sectional Study." Rheumatology International, vol. 38, no. 6, 2018, pp. 1031-1041.
Boutrup RJ, Farup J, Vissing K, et al. Skeletal muscle stem cell characteristics and myonuclei content in patients with rheumatoid arthritis: a cross-sectional study. Rheumatol Int. 2018;38(6):1031-1041.
Boutrup, R. J., Farup, J., Vissing, K., Kjaer, M., & Mikkelsen, U. R. (2018). Skeletal muscle stem cell characteristics and myonuclei content in patients with rheumatoid arthritis: a cross-sectional study. Rheumatology International, 38(6), 1031-1041. https://doi.org/10.1007/s00296-018-4028-y
Boutrup RJ, et al. Skeletal Muscle Stem Cell Characteristics and Myonuclei Content in Patients With Rheumatoid Arthritis: a Cross-sectional Study. Rheumatol Int. 2018;38(6):1031-1041. PubMed PMID: 29651539.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Skeletal muscle stem cell characteristics and myonuclei content in patients with rheumatoid arthritis: a cross-sectional study. AU - Boutrup,Rasmus Jentoft, AU - Farup,Jean, AU - Vissing,Kristian, AU - Kjaer,Michael, AU - Mikkelsen,Ulla Ramer, Y1 - 2018/04/12/ PY - 2018/01/25/received PY - 2018/04/07/accepted PY - 2018/4/14/pubmed PY - 2018/12/12/medline PY - 2018/4/14/entrez KW - Cross-sectional studies KW - Interleukin-6 KW - Rheumatoid arthritis KW - Skeletal muscle KW - Stem cells KW - Tumor necrosis factor-alpha SP - 1031 EP - 1041 JF - Rheumatology international JO - Rheumatol Int VL - 38 IS - 6 N2 - To investigate satellite cells (SCs) and myonuclei characteristics in patients with rheumatoid arthritis (RA). Resting biopsies from m. vastus lateralis were obtained from thirteen RA patients and thirteen matched healthy controls (CON). Muscle biopsies were immunohistochemically stained and analyzed for fiber type specific content of SCs (Pax7+), proliferating SCs (Pax7+/MyoD+) and differentiating SCs (myogenin+). Furthermore, we quantified fiber type specific content of myonuclei and myofiber cross-sectional area (CSA). Finally, newly formed/regenerating fibers expressing neonatal MHC (nMHC+) were determined. The fiber type specific number of SCs did not differ between RA patients and CON, nor did the content of proliferating or differentiating SCs. In contrast, the content of myonuclei per fiber was higher in RA patients than CON for both type I (2.01 ± 0.41 vs. 1.42 ± 0.40 myonuclei/fiber, p < 0.01) and type II fibers (2.01 ± 0.41 vs. 1.37 ± 0.32 myonuclei/fiber, p < 0.01). No differences were observed in fiber composition, fiber type specific CSA or content of nMHC+ fibers. Our results indicate an increased propensity for myogenic differentiation of SC leading to an elevated myonuclear content in the skeletal muscle of RA patients. It is hypothesized that this could be a compensatory regulatory response related to the chronic inflammation in these patients. SN - 1437-160X UR - https://www.unboundmedicine.com/medline/citation/29651539/Skeletal_muscle_stem_cell_characteristics_and_myonuclei_content_in_patients_with_rheumatoid_arthritis:_a_cross_sectional_study_ L2 - https://dx.doi.org/10.1007/s00296-018-4028-y DB - PRIME DP - Unbound Medicine ER -