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Regulation of Fc receptor for IgE (CD23) and class II MHC antigen expression on Burkitt's lymphoma cell lines by human IL-4 and IFN-gamma.
J Immunol. 1988 Apr 15; 140(8):2625-32.JI

Abstract

The effect of rIL-4 on the expression of low affinity receptor for the Fc part of IgE (Fc epsilon R2/CD23) and class II MHC antigens on Burkitt's lymphoma (BL) cell lines was investigated. Some of the BL lines contained low percentages of CD23 and HLA-DQ-positive cells, but virtually all cells expressed HLA-DR. IL-4 induced CD23 and class II MHC Ag expression on 7 of 9 BL. Optimal CD23 and class II MHC expression was observed after 48-72 h of incubation. Induction of CD23 and class II MHC Ag in the BL cell line BL2 by IL-4 was confirmed at the specific mRNA level. Significant activation of HLA-DQ mRNA was obtained after 6 h of incubation with IL-4 and gradually increased during prolonged incubation. Maximal induction of mRNA transcription occurred after 48 to 72 h. Optimal induction of HLA-DR and CD23 transcription in BL2 was also observed after 48 to 72 h. The induction of CD23 and class II MHC Ag seems to be specific for IL-4, because rIL-1, rIL-2, rIFN-gamma, recombinant granulocyte-macrophage-CSF, and a commercial source of low m.w. B cell growth factor were ineffective. In addition, the expression of class I MHC Ag, the transferrin receptor, CD38, CD25, CD10, CD20, and CD21 were not affected by IL-4. Interestingly, IFN-gamma and PGE2 suppressed the IL-4-induced membrane expression of CD23 and class II MHC Ag in a dose-dependent way. IFN-gamma also blocked IL-4-induced CD23 mRNA transcription in BL2 completely, whereas PGE2 (10(-7) M) was partially inhibitory. The induction of CD23 and class II MHC Ag by IL-4 required intact protein synthesis as shown by its inhibition by cycloheximide. These results indicate that the induction of CD23 and class II MHC Ag by IL-4 is regulated in a coordinated way.

Authors+Show Affiliations

UNICET, Laboratory for Immunological Research, Dardilly, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2965726

Citation

Rousset, F, et al. "Regulation of Fc Receptor for IgE (CD23) and Class II MHC Antigen Expression On Burkitt's Lymphoma Cell Lines By Human IL-4 and IFN-gamma." Journal of Immunology (Baltimore, Md. : 1950), vol. 140, no. 8, 1988, pp. 2625-32.
Rousset F, Malefijt RW, Slierendregt B, et al. Regulation of Fc receptor for IgE (CD23) and class II MHC antigen expression on Burkitt's lymphoma cell lines by human IL-4 and IFN-gamma. J Immunol. 1988;140(8):2625-32.
Rousset, F., Malefijt, R. W., Slierendregt, B., Aubry, J. P., Bonnefoy, J. Y., Defrance, T., Banchereau, J., & de Vries, J. E. (1988). Regulation of Fc receptor for IgE (CD23) and class II MHC antigen expression on Burkitt's lymphoma cell lines by human IL-4 and IFN-gamma. Journal of Immunology (Baltimore, Md. : 1950), 140(8), 2625-32.
Rousset F, et al. Regulation of Fc Receptor for IgE (CD23) and Class II MHC Antigen Expression On Burkitt's Lymphoma Cell Lines By Human IL-4 and IFN-gamma. J Immunol. 1988 Apr 15;140(8):2625-32. PubMed PMID: 2965726.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of Fc receptor for IgE (CD23) and class II MHC antigen expression on Burkitt's lymphoma cell lines by human IL-4 and IFN-gamma. AU - Rousset,F, AU - Malefijt,R W, AU - Slierendregt,B, AU - Aubry,J P, AU - Bonnefoy,J Y, AU - Defrance,T, AU - Banchereau,J, AU - de Vries,J E, PY - 1988/4/15/pubmed PY - 1988/4/15/medline PY - 1988/4/15/entrez SP - 2625 EP - 32 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 140 IS - 8 N2 - The effect of rIL-4 on the expression of low affinity receptor for the Fc part of IgE (Fc epsilon R2/CD23) and class II MHC antigens on Burkitt's lymphoma (BL) cell lines was investigated. Some of the BL lines contained low percentages of CD23 and HLA-DQ-positive cells, but virtually all cells expressed HLA-DR. IL-4 induced CD23 and class II MHC Ag expression on 7 of 9 BL. Optimal CD23 and class II MHC expression was observed after 48-72 h of incubation. Induction of CD23 and class II MHC Ag in the BL cell line BL2 by IL-4 was confirmed at the specific mRNA level. Significant activation of HLA-DQ mRNA was obtained after 6 h of incubation with IL-4 and gradually increased during prolonged incubation. Maximal induction of mRNA transcription occurred after 48 to 72 h. Optimal induction of HLA-DR and CD23 transcription in BL2 was also observed after 48 to 72 h. The induction of CD23 and class II MHC Ag seems to be specific for IL-4, because rIL-1, rIL-2, rIFN-gamma, recombinant granulocyte-macrophage-CSF, and a commercial source of low m.w. B cell growth factor were ineffective. In addition, the expression of class I MHC Ag, the transferrin receptor, CD38, CD25, CD10, CD20, and CD21 were not affected by IL-4. Interestingly, IFN-gamma and PGE2 suppressed the IL-4-induced membrane expression of CD23 and class II MHC Ag in a dose-dependent way. IFN-gamma also blocked IL-4-induced CD23 mRNA transcription in BL2 completely, whereas PGE2 (10(-7) M) was partially inhibitory. The induction of CD23 and class II MHC Ag by IL-4 required intact protein synthesis as shown by its inhibition by cycloheximide. These results indicate that the induction of CD23 and class II MHC Ag by IL-4 is regulated in a coordinated way. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/2965726/Regulation_of_Fc_receptor_for_IgE__CD23__and_class_II_MHC_antigen_expression_on_Burkitt's_lymphoma_cell_lines_by_human_IL_4_and_IFN_gamma_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=2965726 DB - PRIME DP - Unbound Medicine ER -