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Development and application of CRISPR/Cas9 technologies in genomic editing.
Hum Mol Genet. 2018 08 01; 27(R2):R79-R88.HM

Abstract

Genomic editing to correct disease-causing mutations is a promising approach for the treatment of human diseases. As a simple and programmable nuclease-based genomic editing tool, the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system has substantially improved the ability to make precise changes in the human genome. Rapid development of CRISPR-based technologies in recent years has expanded its application scope and promoted CRISPR-based therapies in preclinical trails. Here, we review the application of the CRISPR system over the last 2 years; including its development and application in base editing, transcription modulation and epigenetic editing, genomic-scale screening, and cell and embryo therapy. Finally, the prospects and challenges related to application of CRISPR/Cas9 technologies are discussed.

Authors+Show Affiliations

Institute of Cell and Development Biology, College of Life Sciences, Zijingang Campus, Zhejiang University, Hangzhou, Zhejiang, P.R. China.Institute of Orthopedics, Xiaoshan Traditional Chinese Medical Hospital, Hangzhou, Zhejiang, China.Institute of Cell and Development Biology, College of Life Sciences, Zijingang Campus, Zhejiang University, Hangzhou, Zhejiang, P.R. China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

29659822

Citation

Zhang, Cui, et al. "Development and Application of CRISPR/Cas9 Technologies in Genomic Editing." Human Molecular Genetics, vol. 27, no. R2, 2018, pp. R79-R88.
Zhang C, Quan R, Wang J. Development and application of CRISPR/Cas9 technologies in genomic editing. Hum Mol Genet. 2018;27(R2):R79-R88.
Zhang, C., Quan, R., & Wang, J. (2018). Development and application of CRISPR/Cas9 technologies in genomic editing. Human Molecular Genetics, 27(R2), R79-R88. https://doi.org/10.1093/hmg/ddy120
Zhang C, Quan R, Wang J. Development and Application of CRISPR/Cas9 Technologies in Genomic Editing. Hum Mol Genet. 2018 08 1;27(R2):R79-R88. PubMed PMID: 29659822.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and application of CRISPR/Cas9 technologies in genomic editing. AU - Zhang,Cui, AU - Quan,Renfu, AU - Wang,Jinfu, PY - 2018/03/27/received PY - 2018/04/03/accepted PY - 2018/4/17/pubmed PY - 2019/6/18/medline PY - 2018/4/17/entrez SP - R79 EP - R88 JF - Human molecular genetics JO - Hum. Mol. Genet. VL - 27 IS - R2 N2 - Genomic editing to correct disease-causing mutations is a promising approach for the treatment of human diseases. As a simple and programmable nuclease-based genomic editing tool, the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system has substantially improved the ability to make precise changes in the human genome. Rapid development of CRISPR-based technologies in recent years has expanded its application scope and promoted CRISPR-based therapies in preclinical trails. Here, we review the application of the CRISPR system over the last 2 years; including its development and application in base editing, transcription modulation and epigenetic editing, genomic-scale screening, and cell and embryo therapy. Finally, the prospects and challenges related to application of CRISPR/Cas9 technologies are discussed. SN - 1460-2083 UR - https://www.unboundmedicine.com/medline/citation/29659822/Development_and_application_of_CRISPR/Cas9_technologies_in_genomic_editing_ L2 - https://academic.oup.com/hmg/article-lookup/doi/10.1093/hmg/ddy120 DB - PRIME DP - Unbound Medicine ER -