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Crataegus Special Extract WS 1442 Effects on eNOS and microRNA 155.
Planta Med. 2018 Oct; 84(15):1094-1100.PM

Abstract

Increased expression of microRNA 155 (miR-155) results in a decrease in endothelial nitric oxide synthase (eNOS) expression and impaired endothelial function. Factors that have been shown to increase expression of miR-155 may be mitigated by WS 1442, an extract of hawthorn leaves and flowers (Crataegus special extract) that contains a range of pharmacologically active substances including oligomeric proanthocyanidins and flavonoids. The purpose of this study is to determine the effect of WS 1442 on the expression of miR-155 and eNOS in the presence of tumor necrosis factor (TNF-α). Human umbilical vein endothelial cells (HUVECs) were studied after the exposure to TNF-α, with or without simvastatin (positive control) and WS 1442. The expression levels of eNOS, phosphorylated eNOS, and miR-155 in the different HUVEC treatment groups were determined by western blot and quantitative real-time polymerase chain reaction, respectively. To evaluate the effect of WS 1442 on the eNOS activity, the medium and intracellular nitrate/nitrite (NO) concentrations were also analyzed using a colorimetric Griess assay kit. The results demonstrated that TNF-α upregulated miR-155 expression and decreased eNOS expression and NO concentrations. WS 1442 also increased miR-155 expression and decreased eNOS expression but, unlike TNF-α, increased phosphorylated eNOS expression and NO concentrations. Surprisingly, WS 1442 increased miR-155 expression; however, WS 1442 mitigated the overall negative effect of miR-155 on decreasing eNOS expression by increasing expression of phosphorylated eNOS and resulting in an increase in NO concentrations. In the setting where miR-155 may be expressed, WS 1442 may offer vascular protection by increasing the expression of phosphorylated eNOS.

Authors+Show Affiliations

Department of Clinical Pharmacy, College of Pharmacy, University of Michigan, Ann Arbor, Michigan, USA.Department of Clinical Pharmacy, College of Pharmacy, University of Michigan, Ann Arbor, Michigan, USA. The Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, China.Department of Clinical Pharmacy, College of Pharmacy, University of Michigan, Ann Arbor, Michigan, USA.Department of Clinical Pharmacy, College of Pharmacy, University of Michigan, Ann Arbor, Michigan, USA.Department of Pharmacy Practice & Administrative Sciences, College of Pharmacy, University of New Mexico, Albuquerque, New Mexico, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29660753

Citation

Wang, Xinwen, et al. "Crataegus Special Extract WS 1442 Effects On eNOS and microRNA 155." Planta Medica, vol. 84, no. 15, 2018, pp. 1094-1100.
Wang X, Liang Y, Shi J, et al. Crataegus Special Extract WS 1442 Effects on eNOS and microRNA 155. Planta Med. 2018;84(15):1094-1100.
Wang, X., Liang, Y., Shi, J., Zhu, H. J., & Bleske, B. E. (2018). Crataegus Special Extract WS 1442 Effects on eNOS and microRNA 155. Planta Medica, 84(15), 1094-1100. https://doi.org/10.1055/a-0601-7083
Wang X, et al. Crataegus Special Extract WS 1442 Effects On eNOS and microRNA 155. Planta Med. 2018;84(15):1094-1100. PubMed PMID: 29660753.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Crataegus Special Extract WS 1442 Effects on eNOS and microRNA 155. AU - Wang,Xinwen, AU - Liang,Yan, AU - Shi,Jian, AU - Zhu,Hao-Jie, AU - Bleske,Barry E, Y1 - 2018/04/16/ PY - 2018/4/17/pubmed PY - 2018/11/6/medline PY - 2018/4/17/entrez SP - 1094 EP - 1100 JF - Planta medica JO - Planta Med VL - 84 IS - 15 N2 - Increased expression of microRNA 155 (miR-155) results in a decrease in endothelial nitric oxide synthase (eNOS) expression and impaired endothelial function. Factors that have been shown to increase expression of miR-155 may be mitigated by WS 1442, an extract of hawthorn leaves and flowers (Crataegus special extract) that contains a range of pharmacologically active substances including oligomeric proanthocyanidins and flavonoids. The purpose of this study is to determine the effect of WS 1442 on the expression of miR-155 and eNOS in the presence of tumor necrosis factor (TNF-α). Human umbilical vein endothelial cells (HUVECs) were studied after the exposure to TNF-α, with or without simvastatin (positive control) and WS 1442. The expression levels of eNOS, phosphorylated eNOS, and miR-155 in the different HUVEC treatment groups were determined by western blot and quantitative real-time polymerase chain reaction, respectively. To evaluate the effect of WS 1442 on the eNOS activity, the medium and intracellular nitrate/nitrite (NO) concentrations were also analyzed using a colorimetric Griess assay kit. The results demonstrated that TNF-α upregulated miR-155 expression and decreased eNOS expression and NO concentrations. WS 1442 also increased miR-155 expression and decreased eNOS expression but, unlike TNF-α, increased phosphorylated eNOS expression and NO concentrations. Surprisingly, WS 1442 increased miR-155 expression; however, WS 1442 mitigated the overall negative effect of miR-155 on decreasing eNOS expression by increasing expression of phosphorylated eNOS and resulting in an increase in NO concentrations. In the setting where miR-155 may be expressed, WS 1442 may offer vascular protection by increasing the expression of phosphorylated eNOS. SN - 1439-0221 UR - https://www.unboundmedicine.com/medline/citation/29660753/Crataegus_Special_Extract_WS_1442_Effects_on_eNOS_and_microRNA_155_ DB - PRIME DP - Unbound Medicine ER -