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The CpxA/CpxR Two-Component System Affects Biofilm Formation and Virulence in Actinobacillus pleuropneumoniae.

Abstract

Gram-negative bacteria have evolved numerous two-component systems (TCSs) to cope with external environmental changes. The CpxA/CpxR TCS consisting of the kinase CpxA and the regulator CpxR, is known to be involved in the biofilm formation and virulence of Escherichia coli. However, the role of CpxA/CpxR remained unclear in Actinobacillus pleuropneumoniae, a bacterial pathogen that can cause porcine contagious pleuropneumonia (PCP). In this report, we show that CpxA/CpxR contributes to the biofilm formation ability of A. pleuropneumoniae. Furthermore, we demonstrate that CpxA/CpxR plays an important role in the expression of several biofilm-related genes in A. pleuropneumoniae, such as rpoE and pgaC. Furthermore, The results of electrophoretic mobility shift assays (EMSAs) and DNase I footprinting analysis demonstrate that CpxR-P can regulate the expression of the pgaABCD operon through rpoE. In an experimental infection of mice, the animals infected with a cpxA/cpxR mutant exhibited delayed mortality and lower bacterial loads in the lung than those infected with the wildtype bacteria. In conclusion, these results indicate that the CpxA/CpxR TCS plays a contributing role in the biofilm formation and virulence of A. pleuropneumoniae.

Authors+Show Affiliations

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China. The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, China. Key Laboratory of Development of Veterinary Diagnostic Products of Ministry of Agriculture, Huazhong Agricultural University, Wuhan, China.State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China. The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, China. Key Laboratory of Development of Veterinary Diagnostic Products of Ministry of Agriculture, Huazhong Agricultural University, Wuhan, China.State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China. The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, China. Key Laboratory of Development of Veterinary Diagnostic Products of Ministry of Agriculture, Huazhong Agricultural University, Wuhan, China.State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China. The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, China. Key Laboratory of Development of Veterinary Diagnostic Products of Ministry of Agriculture, Huazhong Agricultural University, Wuhan, China.State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China. The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, China. Key Laboratory of Development of Veterinary Diagnostic Products of Ministry of Agriculture, Huazhong Agricultural University, Wuhan, China.State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China. The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, China. Key Laboratory of Development of Veterinary Diagnostic Products of Ministry of Agriculture, Huazhong Agricultural University, Wuhan, China.State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China. The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, China. Key Laboratory of Development of Veterinary Diagnostic Products of Ministry of Agriculture, Huazhong Agricultural University, Wuhan, China.State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China. The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, China. Key Laboratory of Development of Veterinary Diagnostic Products of Ministry of Agriculture, Huazhong Agricultural University, Wuhan, China.Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, China.State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China. The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, China. Key Laboratory of Development of Veterinary Diagnostic Products of Ministry of Agriculture, Huazhong Agricultural University, Wuhan, China.State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China. The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, China. Key Laboratory of Development of Veterinary Diagnostic Products of Ministry of Agriculture, Huazhong Agricultural University, Wuhan, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29662838

Citation

Li, Huan, et al. "The CpxA/CpxR Two-Component System Affects Biofilm Formation and Virulence in Actinobacillus Pleuropneumoniae." Frontiers in Cellular and Infection Microbiology, vol. 8, 2018, p. 72.
Li H, Liu F, Peng W, et al. The CpxA/CpxR Two-Component System Affects Biofilm Formation and Virulence in Actinobacillus pleuropneumoniae. Front Cell Infect Microbiol. 2018;8:72.
Li, H., Liu, F., Peng, W., Yan, K., Zhao, H., Liu, T., Cheng, H., Chang, P., Yuan, F., Chen, H., & Bei, W. (2018). The CpxA/CpxR Two-Component System Affects Biofilm Formation and Virulence in Actinobacillus pleuropneumoniae. Frontiers in Cellular and Infection Microbiology, 8, 72. https://doi.org/10.3389/fcimb.2018.00072
Li H, et al. The CpxA/CpxR Two-Component System Affects Biofilm Formation and Virulence in Actinobacillus Pleuropneumoniae. Front Cell Infect Microbiol. 2018;8:72. PubMed PMID: 29662838.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The CpxA/CpxR Two-Component System Affects Biofilm Formation and Virulence in Actinobacillus pleuropneumoniae. AU - Li,Huan, AU - Liu,Feng, AU - Peng,Wei, AU - Yan,Kang, AU - Zhao,Haixu, AU - Liu,Ting, AU - Cheng,Hui, AU - Chang,Peixi, AU - Yuan,Fangyan, AU - Chen,Huanchun, AU - Bei,Weicheng, Y1 - 2018/03/20/ PY - 2017/12/06/received PY - 2018/02/27/accepted PY - 2018/4/18/entrez PY - 2018/4/18/pubmed PY - 2019/3/26/medline KW - Actinobacillus pleuropneumoniae KW - CpxA/CpxR KW - biofilm KW - rpoE KW - virulence SP - 72 EP - 72 JF - Frontiers in cellular and infection microbiology JO - Front Cell Infect Microbiol VL - 8 N2 - Gram-negative bacteria have evolved numerous two-component systems (TCSs) to cope with external environmental changes. The CpxA/CpxR TCS consisting of the kinase CpxA and the regulator CpxR, is known to be involved in the biofilm formation and virulence of Escherichia coli. However, the role of CpxA/CpxR remained unclear in Actinobacillus pleuropneumoniae, a bacterial pathogen that can cause porcine contagious pleuropneumonia (PCP). In this report, we show that CpxA/CpxR contributes to the biofilm formation ability of A. pleuropneumoniae. Furthermore, we demonstrate that CpxA/CpxR plays an important role in the expression of several biofilm-related genes in A. pleuropneumoniae, such as rpoE and pgaC. Furthermore, The results of electrophoretic mobility shift assays (EMSAs) and DNase I footprinting analysis demonstrate that CpxR-P can regulate the expression of the pgaABCD operon through rpoE. In an experimental infection of mice, the animals infected with a cpxA/cpxR mutant exhibited delayed mortality and lower bacterial loads in the lung than those infected with the wildtype bacteria. In conclusion, these results indicate that the CpxA/CpxR TCS plays a contributing role in the biofilm formation and virulence of A. pleuropneumoniae. SN - 2235-2988 UR - https://www.unboundmedicine.com/medline/citation/29662838/The_CpxA/CpxR_Two_Component_System_Affects_Biofilm_Formation_and_Virulence_in_Actinobacillus_pleuropneumoniae_ L2 - https://doi.org/10.3389/fcimb.2018.00072 DB - PRIME DP - Unbound Medicine ER -