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Ground Beef High in Total Fat and Saturated Fatty Acids Decreases X Receptor Signaling Targets in Peripheral Blood Mononuclear Cells of Men and Women.
Lipids. 2018 03; 53(3):279-290.L

Abstract

We hypothesized that consumption of saturated fatty acids in the form of high-fat ground beef for 5 weeks would depress liver X receptor signaling targets in peripheral blood mononuclear cells (PBMC) and that changes in gene expression would be associated with the corresponding changes in lipoprotein cholesterol (C) concentrations. Older men (n = 5, age 68.0 ± 4.6 years) and postmenopausal women (n = 7, age 60.9 ± 3.1 years) were assigned randomly to consume ground-beef containing 18% total fat (18F) or 25% total fat (25F), five patties per week for 5 weeks with an intervening 4-week washout period. The 25F and 18F ground-beef increased (p < 0.05) the intake of saturated fat, monounsaturated fat, palmitic acid, and stearic acid, but the 25F ground-beef increased only the intake of oleic acid (p < 0.05). The ground-beefs 18F and 25F increased the plasma concentration of palmitic acid (p < 0.05) and decreased the plasma concentrations of arachidonic, eicosapentaenoic, and docosahexaenic acids (p < 0.05). The interventions of 18F and 25F ground-beef decreased very low-density lipoprotein C concentrations and increased particle diameters and low-density lipoprotein (LDL)-I-C and LDL-II-C concentrations (p < 0.05). The ground-beef 25F decreased PBMC mRNA levels for the adenosine triphosphate (ATP) binding cassette A, ATP binding cassette G1, sterol regulatory element binding protein-1, and LDL receptor (LDLR) (p < 0.05). The ground-beef 18F increased mRNA levels for stearoyl-CoA desaturase-1 (p < 0.05). We conclude that the increased LDL particle size and LDL-I-C and LDL-II-C concentrations following the 25F ground-beef intervention may have been caused by decreased hepatic LDLR gene expression.

Authors+Show Affiliations

Department of Animal Science, Chungbuk National University, Cheongju, 362-763 South Korea.Department of Animal Science, 2471 Texas A&M University, College Station, TX, USA.Department of Poultry Science, 2742 Texas A&M University, College Station, TX, USA.Scott and White Clinic, Cardiology, 700 Scott and White Drive, College Station, TX, USA.Department of Animal Science, 2471 Texas A&M University, College Station, TX, USA.

Pub Type(s)

Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29663407

Citation

Choi, Seong H., et al. "Ground Beef High in Total Fat and Saturated Fatty Acids Decreases X Receptor Signaling Targets in Peripheral Blood Mononuclear Cells of Men and Women." Lipids, vol. 53, no. 3, 2018, pp. 279-290.
Choi SH, Gharahmany G, Walzem RL, et al. Ground Beef High in Total Fat and Saturated Fatty Acids Decreases X Receptor Signaling Targets in Peripheral Blood Mononuclear Cells of Men and Women. Lipids. 2018;53(3):279-290.
Choi, S. H., Gharahmany, G., Walzem, R. L., Meade, T. H., & Smith, S. B. (2018). Ground Beef High in Total Fat and Saturated Fatty Acids Decreases X Receptor Signaling Targets in Peripheral Blood Mononuclear Cells of Men and Women. Lipids, 53(3), 279-290. https://doi.org/10.1002/lipd.12028
Choi SH, et al. Ground Beef High in Total Fat and Saturated Fatty Acids Decreases X Receptor Signaling Targets in Peripheral Blood Mononuclear Cells of Men and Women. Lipids. 2018;53(3):279-290. PubMed PMID: 29663407.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ground Beef High in Total Fat and Saturated Fatty Acids Decreases X Receptor Signaling Targets in Peripheral Blood Mononuclear Cells of Men and Women. AU - Choi,Seong H, AU - Gharahmany,Ghazal, AU - Walzem,Rosemary L, AU - Meade,Thomas H, AU - Smith,Stephen B, Y1 - 2018/04/16/ PY - 2017/05/23/received PY - 2017/12/20/revised PY - 2018/01/22/accepted PY - 2018/4/18/pubmed PY - 2019/4/9/medline PY - 2018/4/18/entrez KW - Gene expression KW - Ground beef KW - HDL-cholesterol KW - LDL-cholesterol KW - Peripheral mononuclear blood cells KW - Saturated fatty acids SP - 279 EP - 290 JF - Lipids JO - Lipids VL - 53 IS - 3 N2 - We hypothesized that consumption of saturated fatty acids in the form of high-fat ground beef for 5 weeks would depress liver X receptor signaling targets in peripheral blood mononuclear cells (PBMC) and that changes in gene expression would be associated with the corresponding changes in lipoprotein cholesterol (C) concentrations. Older men (n = 5, age 68.0 ± 4.6 years) and postmenopausal women (n = 7, age 60.9 ± 3.1 years) were assigned randomly to consume ground-beef containing 18% total fat (18F) or 25% total fat (25F), five patties per week for 5 weeks with an intervening 4-week washout period. The 25F and 18F ground-beef increased (p < 0.05) the intake of saturated fat, monounsaturated fat, palmitic acid, and stearic acid, but the 25F ground-beef increased only the intake of oleic acid (p < 0.05). The ground-beefs 18F and 25F increased the plasma concentration of palmitic acid (p < 0.05) and decreased the plasma concentrations of arachidonic, eicosapentaenoic, and docosahexaenic acids (p < 0.05). The interventions of 18F and 25F ground-beef decreased very low-density lipoprotein C concentrations and increased particle diameters and low-density lipoprotein (LDL)-I-C and LDL-II-C concentrations (p < 0.05). The ground-beef 25F decreased PBMC mRNA levels for the adenosine triphosphate (ATP) binding cassette A, ATP binding cassette G1, sterol regulatory element binding protein-1, and LDL receptor (LDLR) (p < 0.05). The ground-beef 18F increased mRNA levels for stearoyl-CoA desaturase-1 (p < 0.05). We conclude that the increased LDL particle size and LDL-I-C and LDL-II-C concentrations following the 25F ground-beef intervention may have been caused by decreased hepatic LDLR gene expression. SN - 1558-9307 UR - https://www.unboundmedicine.com/medline/citation/29663407/Ground_Beef_High_in_Total_Fat_and_Saturated_Fatty_Acids_Decreases_X_Receptor_Signaling_Targets_in_Peripheral_Blood_Mononuclear_Cells_of_Men_and_Women_ L2 - https://doi.org/10.1002/lipd.12028 DB - PRIME DP - Unbound Medicine ER -