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miR-362-3p regulates cell proliferation, migration and invasion of trophoblastic cells under hypoxia through targeting Pax3.
Biomed Pharmacother 2018; 99:462-468BP

Abstract

Preeclampsia (PE), a common obstetrical disorder, is one of the leading causes of pregnancy associated death. PE is closely linked with impaired migration and invasion ability of trophoblastic cells. miR-362-3p recently received our particular attention due not only to its aberrant expression in the placentas of patients with PE, but also to its important roles in regulating migration and invasion of various cells. This study was thus conducted to investigate the roles of miR-362-3p in PE and the related mechanism. The expression of miR-362-3p and Pax3 was examined in placentas of patients with PE and in normal placentas. HTR8/SVneo cells were cultured under hypoxia and transfected with miR-362-3p mimics, miR-362-3p inhibitors or Pax3 over-expression vectors. Results showed up-regulation of miR-362-3p but down-regulation of Pax3 in placentas of preeclamptic pregnancies. Luciferase report assay confirmed that Pax3 is a direct target of miR-362-3p. Although Pax3 was predicted to be targeted by miR-30a-3p and miR-181a-5p as well, their expression either had no difference between placentas of PE patients and normal placentas or showed less increment in placentas of PE patients than miR-362-3p. Exposure to hypoxia inhibited cell viability, migration and invasion of HTR8/SVneo cells. Increasing miR-362-3p by the mimics conferred improved effects on the inhibition. However, deletion of miR-362-3p or overexpression of Pax3 abolished the inhibiton. These results suggest that miR-362-3p/Pax3 axis regulates cell viability, migration and invasion of HTR8/SVneo cells under hypoxia. The present study adds to the further understanding of the pathogenesis of PE.

Authors+Show Affiliations

Department of Obstetrics, The Third Xiangya Hospital of Central South University, Changsha, Hunan, 410013, PR China.Department of Obstetrics and Gynecology, Wuxi Matemal and Child Health Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu Province, 214002, PR China. Electronic address: 15161506909@163.com.Department of Obstetrics and Gynecology, Wuxi Matemal and Child Health Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu Province, 214002, PR China.Department of Obstetrics and Gynecology, Wuxi Matemal and Child Health Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu Province, 214002, PR China.Department of Obstetrics and Gynecology, Wuxi Matemal and Child Health Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu Province, 214002, PR China.Department of Obstetrics and Gynecology, Wuxi Matemal and Child Health Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu Province, 214002, PR China.Department of Obstetrics and Gynecology, Wuxi Matemal and Child Health Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu Province, 214002, PR China.Department of Obstetrics, The Third Xiangya Hospital of Central South University, Changsha, Hunan, 410013, PR China.Department of Obstetrics and Gynecology, Wuxi Matemal and Child Health Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu Province, 214002, PR China. Electronic address: 13600182648@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29665647

Citation

Wang, Nan, et al. "MiR-362-3p Regulates Cell Proliferation, Migration and Invasion of Trophoblastic Cells Under Hypoxia Through Targeting Pax3." Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, vol. 99, 2018, pp. 462-468.
Wang N, Feng Y, Xu J, et al. MiR-362-3p regulates cell proliferation, migration and invasion of trophoblastic cells under hypoxia through targeting Pax3. Biomed Pharmacother. 2018;99:462-468.
Wang, N., Feng, Y., Xu, J., Zou, J., Chen, M., He, Y., ... Gu, Y. (2018). MiR-362-3p regulates cell proliferation, migration and invasion of trophoblastic cells under hypoxia through targeting Pax3. Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, 99, pp. 462-468. doi:10.1016/j.biopha.2018.01.089.
Wang N, et al. MiR-362-3p Regulates Cell Proliferation, Migration and Invasion of Trophoblastic Cells Under Hypoxia Through Targeting Pax3. Biomed Pharmacother. 2018;99:462-468. PubMed PMID: 29665647.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - miR-362-3p regulates cell proliferation, migration and invasion of trophoblastic cells under hypoxia through targeting Pax3. AU - Wang,Nan, AU - Feng,Yaling, AU - Xu,Jianjuan, AU - Zou,Jinfang, AU - Chen,Minghua, AU - He,Yue, AU - Liu,Huan, AU - Xue,Min, AU - Gu,Yanfang, Y1 - 2018/02/20/ PY - 2017/11/06/received PY - 2018/01/08/revised PY - 2018/01/16/accepted PY - 2018/4/19/entrez PY - 2018/4/19/pubmed PY - 2018/9/18/medline KW - Invasion KW - Migration KW - Pax3 KW - Trophoblastic cells KW - miR-362-3p SP - 462 EP - 468 JF - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie JO - Biomed. Pharmacother. VL - 99 N2 - Preeclampsia (PE), a common obstetrical disorder, is one of the leading causes of pregnancy associated death. PE is closely linked with impaired migration and invasion ability of trophoblastic cells. miR-362-3p recently received our particular attention due not only to its aberrant expression in the placentas of patients with PE, but also to its important roles in regulating migration and invasion of various cells. This study was thus conducted to investigate the roles of miR-362-3p in PE and the related mechanism. The expression of miR-362-3p and Pax3 was examined in placentas of patients with PE and in normal placentas. HTR8/SVneo cells were cultured under hypoxia and transfected with miR-362-3p mimics, miR-362-3p inhibitors or Pax3 over-expression vectors. Results showed up-regulation of miR-362-3p but down-regulation of Pax3 in placentas of preeclamptic pregnancies. Luciferase report assay confirmed that Pax3 is a direct target of miR-362-3p. Although Pax3 was predicted to be targeted by miR-30a-3p and miR-181a-5p as well, their expression either had no difference between placentas of PE patients and normal placentas or showed less increment in placentas of PE patients than miR-362-3p. Exposure to hypoxia inhibited cell viability, migration and invasion of HTR8/SVneo cells. Increasing miR-362-3p by the mimics conferred improved effects on the inhibition. However, deletion of miR-362-3p or overexpression of Pax3 abolished the inhibiton. These results suggest that miR-362-3p/Pax3 axis regulates cell viability, migration and invasion of HTR8/SVneo cells under hypoxia. The present study adds to the further understanding of the pathogenesis of PE. SN - 1950-6007 UR - https://www.unboundmedicine.com/medline/citation/29665647/miR_362_3p_regulates_cell_proliferation_migration_and_invasion_of_trophoblastic_cells_under_hypoxia_through_targeting_Pax3_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0753-3322(17)35875-4 DB - PRIME DP - Unbound Medicine ER -