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Exposure to concentrated ambient PM2.5 alters the composition of gut microbiota in a murine model.
Part Fibre Toxicol. 2018 04 17; 15(1):17.PF

Abstract

BACKGROUND

Exposure to ambient fine particulate matter (PM2.5) correlates with abnormal glucose homeostasis, but the underlying biological mechanism has not been fully understood. The gut microbiota is an emerging crucial player in the homeostatic regulation of glucose metabolism. Few studies have investigated its role in the PM2.5 exposure-induced abnormalities in glucose homeostasis.

METHODS

C57Bl/6J mice were exposed to filtered air (FA) or concentrated ambient PM2.5 (CAP) for 12 months using a versatile aerosol concentration enrichment system (VACES) that was modified for long-term whole-body exposures. Their glucose homeostasis and gut microbiota were examined and analysed by correlation and mediation analysis.

RESULTS

Intraperitoneal glucose tolerance test (IPGTT) and insulin tolerance test (ITT) showed that CAP exposure markedly impaired their glucose and insulin tolerance. Faecal microbiota analysis demonstrated that the impairment in glucose homeostasis was coincided with decreased faecal bacterial ACE and Chao-1 estimators (the indexes of community richness), while there was no significant change in all faecal fungal alpha diversity estimators. The Pearson's correlation analyses showed that the bacterial richness estimators were correlated with glucose and insulin tolerance, and the mediation analyses displayed a significant mediation of CAP exposure-induced glucose intolerance by the alteration in the bacterial Chao-1 estimator. LEfSe analyses revealed 24 bacterial and 21 fungal taxa differential between CAP- and FA-exposed animals. Of these, 14 and 20 bacterial taxa were correlated with IPGTT AUC and ITT AUC, respectively, and 5 fungal taxa were correlated with abnormalities in glucose metabolism.

CONCLUSIONS

Chronic exposure to PM2.5 causes gut dysbiosis and may subsequently contribute to the development of abnormalities in glucose metabolism.

Authors+Show Affiliations

Department of Environmental Health, School of Public Health, Fudan University, 130 Dong'an Rd, Shanghai, 200032, China.Shanghai Key Laboratory of Meteorology and Health, Shanghai Meteorological Service, Shanghai, China.Department of Medicine Cardiology Division, School of Medicine, University of Maryland, 20 Penn St. HSFII S022, Baltimore, MD, 21201, USA.Department of Environmental Health, School of Public Health, Fudan University, 130 Dong'an Rd, Shanghai, 200032, China.Department of Interventional & Vascular Surgery, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.Reproductive and Developmental Research Institute of Fudan University, Shanghai, China.Department of Diagnostic Radiology and Nuclear Medicine, University of Maryland School of Medicine, Baltimore, MD, USA.Department of Environmental Health, School of Public Health, Fudan University, 130 Dong'an Rd, Shanghai, 200032, China. Shanghai Key Laboratory of Meteorology and Health, Shanghai Meteorological Service, Shanghai, China.Department of Environmental Health, School of Public Health, Fudan University, 130 Dong'an Rd, Shanghai, 200032, China. yanyi_xu@fudan.edu.cn. Shanghai Key Laboratory of Meteorology and Health, Shanghai Meteorological Service, Shanghai, China. yanyi_xu@fudan.edu.cn.Department of Environmental Health, School of Public Health, Fudan University, 130 Dong'an Rd, Shanghai, 200032, China. yingzhekang@hotmail.com. Shanghai Key Laboratory of Meteorology and Health, Shanghai Meteorological Service, Shanghai, China. yingzhekang@hotmail.com. Department of Medicine Cardiology Division, School of Medicine, University of Maryland, 20 Penn St. HSFII S022, Baltimore, MD, 21201, USA. yingzhekang@hotmail.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29665823

Citation

Wang, Wanjun, et al. "Exposure to Concentrated Ambient PM2.5 Alters the Composition of Gut Microbiota in a Murine Model." Particle and Fibre Toxicology, vol. 15, no. 1, 2018, p. 17.
Wang W, Zhou J, Chen M, et al. Exposure to concentrated ambient PM2.5 alters the composition of gut microbiota in a murine model. Part Fibre Toxicol. 2018;15(1):17.
Wang, W., Zhou, J., Chen, M., Huang, X., Xie, X., Li, W., Cao, Q., Kan, H., Xu, Y., & Ying, Z. (2018). Exposure to concentrated ambient PM2.5 alters the composition of gut microbiota in a murine model. Particle and Fibre Toxicology, 15(1), 17. https://doi.org/10.1186/s12989-018-0252-6
Wang W, et al. Exposure to Concentrated Ambient PM2.5 Alters the Composition of Gut Microbiota in a Murine Model. Part Fibre Toxicol. 2018 04 17;15(1):17. PubMed PMID: 29665823.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Exposure to concentrated ambient PM2.5 alters the composition of gut microbiota in a murine model. AU - Wang,Wanjun, AU - Zhou,Ji, AU - Chen,Minjie, AU - Huang,Xingke, AU - Xie,Xiaoyun, AU - Li,Weihua, AU - Cao,Qi, AU - Kan,Haidong, AU - Xu,Yanyi, AU - Ying,Zhekang, Y1 - 2018/04/17/ PY - 2017/12/16/received PY - 2018/03/29/accepted PY - 2018/4/19/entrez PY - 2018/4/19/pubmed PY - 2018/10/16/medline KW - Diabetes KW - Glucose homeostatic KW - Gut microbiota KW - PM2.5 SP - 17 EP - 17 JF - Particle and fibre toxicology JO - Part Fibre Toxicol VL - 15 IS - 1 N2 - BACKGROUND: Exposure to ambient fine particulate matter (PM2.5) correlates with abnormal glucose homeostasis, but the underlying biological mechanism has not been fully understood. The gut microbiota is an emerging crucial player in the homeostatic regulation of glucose metabolism. Few studies have investigated its role in the PM2.5 exposure-induced abnormalities in glucose homeostasis. METHODS: C57Bl/6J mice were exposed to filtered air (FA) or concentrated ambient PM2.5 (CAP) for 12 months using a versatile aerosol concentration enrichment system (VACES) that was modified for long-term whole-body exposures. Their glucose homeostasis and gut microbiota were examined and analysed by correlation and mediation analysis. RESULTS: Intraperitoneal glucose tolerance test (IPGTT) and insulin tolerance test (ITT) showed that CAP exposure markedly impaired their glucose and insulin tolerance. Faecal microbiota analysis demonstrated that the impairment in glucose homeostasis was coincided with decreased faecal bacterial ACE and Chao-1 estimators (the indexes of community richness), while there was no significant change in all faecal fungal alpha diversity estimators. The Pearson's correlation analyses showed that the bacterial richness estimators were correlated with glucose and insulin tolerance, and the mediation analyses displayed a significant mediation of CAP exposure-induced glucose intolerance by the alteration in the bacterial Chao-1 estimator. LEfSe analyses revealed 24 bacterial and 21 fungal taxa differential between CAP- and FA-exposed animals. Of these, 14 and 20 bacterial taxa were correlated with IPGTT AUC and ITT AUC, respectively, and 5 fungal taxa were correlated with abnormalities in glucose metabolism. CONCLUSIONS: Chronic exposure to PM2.5 causes gut dysbiosis and may subsequently contribute to the development of abnormalities in glucose metabolism. SN - 1743-8977 UR - https://www.unboundmedicine.com/medline/citation/29665823/Exposure_to_concentrated_ambient_PM2_5_alters_the_composition_of_gut_microbiota_in_a_murine_model_ L2 - https://particleandfibretoxicology.biomedcentral.com/articles/10.1186/s12989-018-0252-6 DB - PRIME DP - Unbound Medicine ER -