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Effect of Magnesium Acetyltaurate and Taurine on Endothelin1-Induced Retinal Nitrosative Stress in Rats.
Curr Eye Res. 2018 08; 43(8):1032-1040.CE

Abstract

PURPOSE

Retinal ganglion cell apoptosis in glaucoma is associated with elevated levels of endothelin-1 (ET1), a potent vasoconstrictor. ET1-induced retinal ischemia leads to altered expression of nitric oxide synthase (NOS) isoforms leading to increased formation of nitric oxide (NO) and retinal nitrosative stress. Since magnesium (Mg) is known to improve endothelial functions and reduce oxidative stress and taurine (TAU) possesses potent antioxidant properties, we investigated the protective effects of magnesium acetyltaurate (MgAT) against ET1-induced nitrosative stress and retinal damage in rats. We also compared the effects of MgAT with that of TAU alone.

METHODS

Sprague Dawley rats were intravitreally injected with ET1. MgAT and TAU were administered as pre-, co-, or posttreatment. Subsequently, the expression of NOS isoforms was detected in retina by immunohistochemistry, retinal nitrotyrosine level was estimated using ELISA, and retinal cell apoptosis was detected by TUNEL staining.

RESULTS

Intravitreal ET1 caused a significant increase in the expressions of nNOS and iNOS while eNOS expression was significantly reduced compared to vehicle treated group. Administration of both MgAT and TAU restored the altered levels of NOS isoform expression, reduced retinal nitrosative stress and retinal cell apoptosis. The effect of MgAT, however, was greater than that of TAU alone.

CONCLUSIONS

MgAT and TAU prevent ET1-induced retinal cell apoptosis by reducing retinal nitrosative stress in Sprague Dawley rats. Addition of TAU to Mg seems to enhance the efficacy of TAU compared to when given alone. Moreover, the pretreatment with MgAT/TAU showed higher efficacy compared to co- or posttreatment.

Authors+Show Affiliations

a Center for Neuroscience Research, Faculty of Medicine , Universiti Teknologi MARA Sungai Buloh Campus , Selangor , Malaysia.a Center for Neuroscience Research, Faculty of Medicine , Universiti Teknologi MARA Sungai Buloh Campus , Selangor , Malaysia.a Center for Neuroscience Research, Faculty of Medicine , Universiti Teknologi MARA Sungai Buloh Campus , Selangor , Malaysia. b Volgograd State Medical University, Research Institute of Pharmacology , Volgograd , Russia.c Faculty of Medicine, International Medical University , IMU Clinical School , Seremban , Malaysia.a Center for Neuroscience Research, Faculty of Medicine , Universiti Teknologi MARA Sungai Buloh Campus , Selangor , Malaysia.b Volgograd State Medical University, Research Institute of Pharmacology , Volgograd , Russia.b Volgograd State Medical University, Research Institute of Pharmacology , Volgograd , Russia.a Center for Neuroscience Research, Faculty of Medicine , Universiti Teknologi MARA Sungai Buloh Campus , Selangor , Malaysia.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29676937

Citation

Nor Arfuzir, Natasha Najwa, et al. "Effect of Magnesium Acetyltaurate and Taurine On Endothelin1-Induced Retinal Nitrosative Stress in Rats." Current Eye Research, vol. 43, no. 8, 2018, pp. 1032-1040.
Nor Arfuzir NN, Agarwal R, Iezhitsa I, et al. Effect of Magnesium Acetyltaurate and Taurine on Endothelin1-Induced Retinal Nitrosative Stress in Rats. Curr Eye Res. 2018;43(8):1032-1040.
Nor Arfuzir, N. N., Agarwal, R., Iezhitsa, I., Agarwal, P., Sidek, S., Spasov, A., Ozerov, A., & Mohd Ismail, N. (2018). Effect of Magnesium Acetyltaurate and Taurine on Endothelin1-Induced Retinal Nitrosative Stress in Rats. Current Eye Research, 43(8), 1032-1040. https://doi.org/10.1080/02713683.2018.1467933
Nor Arfuzir NN, et al. Effect of Magnesium Acetyltaurate and Taurine On Endothelin1-Induced Retinal Nitrosative Stress in Rats. Curr Eye Res. 2018;43(8):1032-1040. PubMed PMID: 29676937.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of Magnesium Acetyltaurate and Taurine on Endothelin1-Induced Retinal Nitrosative Stress in Rats. AU - Nor Arfuzir,Natasha Najwa, AU - Agarwal,Renu, AU - Iezhitsa,Igor, AU - Agarwal,Puneet, AU - Sidek,Sabrilhakim, AU - Spasov,Alexander, AU - Ozerov,Alexander, AU - Mohd Ismail,Nafeeza, Y1 - 2018/05/08/ PY - 2018/4/21/pubmed PY - 2019/5/22/medline PY - 2018/4/21/entrez KW - Retina KW - endothelin-1 KW - magnesium acetyltaurate KW - nitrosative stress KW - taurine SP - 1032 EP - 1040 JF - Current eye research JO - Curr Eye Res VL - 43 IS - 8 N2 - PURPOSE: Retinal ganglion cell apoptosis in glaucoma is associated with elevated levels of endothelin-1 (ET1), a potent vasoconstrictor. ET1-induced retinal ischemia leads to altered expression of nitric oxide synthase (NOS) isoforms leading to increased formation of nitric oxide (NO) and retinal nitrosative stress. Since magnesium (Mg) is known to improve endothelial functions and reduce oxidative stress and taurine (TAU) possesses potent antioxidant properties, we investigated the protective effects of magnesium acetyltaurate (MgAT) against ET1-induced nitrosative stress and retinal damage in rats. We also compared the effects of MgAT with that of TAU alone. METHODS: Sprague Dawley rats were intravitreally injected with ET1. MgAT and TAU were administered as pre-, co-, or posttreatment. Subsequently, the expression of NOS isoforms was detected in retina by immunohistochemistry, retinal nitrotyrosine level was estimated using ELISA, and retinal cell apoptosis was detected by TUNEL staining. RESULTS: Intravitreal ET1 caused a significant increase in the expressions of nNOS and iNOS while eNOS expression was significantly reduced compared to vehicle treated group. Administration of both MgAT and TAU restored the altered levels of NOS isoform expression, reduced retinal nitrosative stress and retinal cell apoptosis. The effect of MgAT, however, was greater than that of TAU alone. CONCLUSIONS: MgAT and TAU prevent ET1-induced retinal cell apoptosis by reducing retinal nitrosative stress in Sprague Dawley rats. Addition of TAU to Mg seems to enhance the efficacy of TAU compared to when given alone. Moreover, the pretreatment with MgAT/TAU showed higher efficacy compared to co- or posttreatment. SN - 1460-2202 UR - https://www.unboundmedicine.com/medline/citation/29676937/Effect_of_Magnesium_Acetyltaurate_and_Taurine_on_Endothelin1_Induced_Retinal_Nitrosative_Stress_in_Rats_ L2 - https://www.tandfonline.com/doi/full/10.1080/02713683.2018.1467933 DB - PRIME DP - Unbound Medicine ER -