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Ameliorating effect of berbamine on hepatic key enzymes of carbohydrate metabolism in high-fat diet and streptozotocin induced type 2 diabetic rats.
Biomed Pharmacother. 2018 Jul; 103:539-545.BP

Abstract

BACKGROUND

Aberrations in the activities of key enzymes of carbohydrate metabolism is well documented in diabetes mellitus. Previous studies have shown that active ingredients in the extracts of Berberis aristata exhibits diverse pharmacological activities in animal models.

OBJECTIVE

The present study was undertaken to investigate whether berbamine (BBM), an alkaloid from the roots of Berberis aristata can ameliorate the altered activities of carbohydrate metabolic enzymes in high fat diet (HFD)/streptozotocin (STZ) induced diabetic rats.

RESULTS

Supplementation of HFD for 4 weeks followed by intraperitonial administration of single low dose of STZ (40 mg/kg b.w.) to Sprague Dawley rats resulted in significant hyperglycemia with a decline in plasma insulin levels. The rats also exhibited decreased hemoglobin with an increase in glycated hemoglobin levels. The activities of hexokinase, glucose-6-phosphate dehydrogenase were decreased whereas increases in the activities of glucose-6-phosphatase and fructose-1,6-bisphosphatase were observed in the hepatic tissues of diabetic control rats. Glycogen content in the hepatic and skeletal muscle tissues were found to be decreased in diabetic rats. Oral administration of BBM for 56 days, dose dependently (50, 100, 200 mg/kg b.w.) improved insulin secretion in diabetic treated rats. Immunohistochemical studies on pancreas revealed a strong immunoreactivity to insulin in BBM treated rats. At the effective dose of 100 mg/kg b.w., BBM restored the altered activities of carbohydrate metabolic enzymes and also improved glycogen content in insulin dependent tissues.

CONCLUSION

From the biochemical and histochemical data obtained in this study we conclude that BBM ameliorated the activities of metabolic enzymes and maintained glucose homeostasis in HFD/STZ induced diabetic rats and it can be used as a potential phytomedicine for the management of diabetes mellitus.

Authors+Show Affiliations

Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar, 608002, Tamilnadu, India. Electronic address: sankarhari05@gmail.com.Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar, 608002, Tamilnadu, India.Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar, 608002, Tamilnadu, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29677540

Citation

Sankaranarayanan, Chandrasekaran, et al. "Ameliorating Effect of Berbamine On Hepatic Key Enzymes of Carbohydrate Metabolism in High-fat Diet and Streptozotocin Induced Type 2 Diabetic Rats." Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, vol. 103, 2018, pp. 539-545.
Sankaranarayanan C, Nishanthi R, Pugalendi P. Ameliorating effect of berbamine on hepatic key enzymes of carbohydrate metabolism in high-fat diet and streptozotocin induced type 2 diabetic rats. Biomed Pharmacother. 2018;103:539-545.
Sankaranarayanan, C., Nishanthi, R., & Pugalendi, P. (2018). Ameliorating effect of berbamine on hepatic key enzymes of carbohydrate metabolism in high-fat diet and streptozotocin induced type 2 diabetic rats. Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, 103, 539-545. https://doi.org/10.1016/j.biopha.2018.04.066
Sankaranarayanan C, Nishanthi R, Pugalendi P. Ameliorating Effect of Berbamine On Hepatic Key Enzymes of Carbohydrate Metabolism in High-fat Diet and Streptozotocin Induced Type 2 Diabetic Rats. Biomed Pharmacother. 2018;103:539-545. PubMed PMID: 29677540.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ameliorating effect of berbamine on hepatic key enzymes of carbohydrate metabolism in high-fat diet and streptozotocin induced type 2 diabetic rats. AU - Sankaranarayanan,Chandrasekaran, AU - Nishanthi,Ramajayam, AU - Pugalendi,Pachaiappan, Y1 - 2018/04/24/ PY - 2018/01/11/received PY - 2018/04/09/revised PY - 2018/04/09/accepted PY - 2018/4/21/pubmed PY - 2018/10/10/medline PY - 2018/4/21/entrez KW - Berbamine KW - Carbohydrate metabolic enzymes KW - High fat diet KW - Sprague Dawley rats KW - Streptozotocin SP - 539 EP - 545 JF - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie JO - Biomed Pharmacother VL - 103 N2 - BACKGROUND: Aberrations in the activities of key enzymes of carbohydrate metabolism is well documented in diabetes mellitus. Previous studies have shown that active ingredients in the extracts of Berberis aristata exhibits diverse pharmacological activities in animal models. OBJECTIVE: The present study was undertaken to investigate whether berbamine (BBM), an alkaloid from the roots of Berberis aristata can ameliorate the altered activities of carbohydrate metabolic enzymes in high fat diet (HFD)/streptozotocin (STZ) induced diabetic rats. RESULTS: Supplementation of HFD for 4 weeks followed by intraperitonial administration of single low dose of STZ (40 mg/kg b.w.) to Sprague Dawley rats resulted in significant hyperglycemia with a decline in plasma insulin levels. The rats also exhibited decreased hemoglobin with an increase in glycated hemoglobin levels. The activities of hexokinase, glucose-6-phosphate dehydrogenase were decreased whereas increases in the activities of glucose-6-phosphatase and fructose-1,6-bisphosphatase were observed in the hepatic tissues of diabetic control rats. Glycogen content in the hepatic and skeletal muscle tissues were found to be decreased in diabetic rats. Oral administration of BBM for 56 days, dose dependently (50, 100, 200 mg/kg b.w.) improved insulin secretion in diabetic treated rats. Immunohistochemical studies on pancreas revealed a strong immunoreactivity to insulin in BBM treated rats. At the effective dose of 100 mg/kg b.w., BBM restored the altered activities of carbohydrate metabolic enzymes and also improved glycogen content in insulin dependent tissues. CONCLUSION: From the biochemical and histochemical data obtained in this study we conclude that BBM ameliorated the activities of metabolic enzymes and maintained glucose homeostasis in HFD/STZ induced diabetic rats and it can be used as a potential phytomedicine for the management of diabetes mellitus. SN - 1950-6007 UR - https://www.unboundmedicine.com/medline/citation/29677540/Ameliorating_effect_of_berbamine_on_hepatic_key_enzymes_of_carbohydrate_metabolism_in_high_fat_diet_and_streptozotocin_induced_type_2_diabetic_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0753-3322(18)30243-9 DB - PRIME DP - Unbound Medicine ER -