TY - JOUR T1 - Simultaneous analysis of multiple urinary biomarkers for the evaluation of oxidative stress by automated online in-tube solid-phase microextraction coupled with negative/positive ion-switching mode liquid chromatography-tandem mass spectrometry. AU - Saito,Akihiro, AU - Hamano,Mariko, AU - Kataoka,Hiroyuki, Y1 - 2018/05/21/ PY - 2018/02/11/received PY - 2018/04/07/revised PY - 2018/04/10/accepted PY - 2018/4/22/pubmed PY - 2018/10/20/medline PY - 2018/4/22/entrez KW - biomarkers KW - in-tube solid-phase microextraction KW - liquid chromatography KW - reactive oxygen species KW - tandem mass spectrometry SP - 2743 EP - 2749 JF - Journal of separation science JO - J Sep Sci VL - 41 IS - 13 N2 - This study described an automated online method for the simultaneous determination of 8-isoprostane, 8-hydroxy-2'-deoxyguanosine, and 3-nitro-l-tyrosine in human urine. The method involves in-tube solid-phase microextraction using a Carboxen 1006 PLOT capillary column as an extraction device, followed by liquid chromatography with tandem mass spectrometry using a CX column and detection in the negative/positive switching ion-mode by multiple reaction monitoring. Using their stable isotope-labeled internal standards, each of these oxidative stress biomarkers showed good linearity from 0.02 to 2.0 ng/mL. Their detection limits (S/N = 3) were 3.4-21.5 pg/mL, and their intra- and inter-day precisions (relative standard deviations) were >3.9 and 6.5% (n = 5), respectively. This method was applied successfully to the analysis of urine samples, without any other pretreatment and interference peaks. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/29679453/Simultaneous_analysis_of_multiple_urinary_biomarkers_for_the_evaluation_of_oxidative_stress_by_automated_online_in_tube_solid_phase_microextraction_coupled_with_negative/positive_ion_switching_mode_liquid_chromatography_tandem_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -