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A complicated complex: Ion channels, voltage sensing, cell membranes and peptide inhibitors.
Neurosci Lett. 2018 07 13; 679:35-47.NL

Abstract

Voltage-gated ion channels (VGICs) are specialised ion channels that have a voltage dependent mode of action, where ion conduction, or gating, is controlled by a voltage-sensing mechanism. VGICs are critical for electrical signalling and are therefore important pharmacological targets. Among these, voltage-gated sodium channels (NaVs) have attracted particular attention as potential analgesic targets. NaVs, however, comprise several structurally similar subtypes with unique localisations and distinct functions, ranging from amplification of action potentials in nociception (e.g. NaV1.7) to controlling electrical signalling in cardiac function (NaV1.5). Understanding the structural basis of NaV function is therefore of great significance, both to our knowledge of electrical signalling and in development of subtype and state selective drugs. An important tool in this pursuit has been the use of peptides from animal venoms as selective NaV modulators. In this review, we look at peptides, particularly from spider venoms, that inhibit NaVs by binding to the voltage sensing domain (VSD) of this channel, known as gating modifier toxins (GMT). In the first part of the review, we look at the structural determinants of voltage sensing in VGICs, the gating cycle and the conformational changes that accompany VSD movement. Next, the modulation of the analgesic target NaV1.7 by GMTs is reviewed to develop bioinformatic tools that, based on sequence information alone, can identify toxins that are likely to inhibit this channel. The same approach is also used to define VSD sequences, other than that from NaV1.7, which are likely to be sensitive to this class of toxins. The final section of the review focuses on the important role of the cellular membrane in channel modulation and also how the lipid composition affects measurements of peptide-channel interactions both in binding kinetics measurements in solution and in cell-based functional assays.

Authors+Show Affiliations

Centre for Advanced Imaging, The University of Queensland, Australia.Centre for Advanced Imaging, The University of Queensland, Australia.Centre for Advanced Imaging, The University of Queensland, Australia.Centre for Advanced Imaging, The University of Queensland, Australia.Centre for Advanced Imaging, The University of Queensland, Australia. Electronic address: m.mobli@uq.edu.au.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

29684532

Citation

Zhang, Alan H., et al. "A Complicated Complex: Ion Channels, Voltage Sensing, Cell Membranes and Peptide Inhibitors." Neuroscience Letters, vol. 679, 2018, pp. 35-47.
Zhang AH, Sharma G, Undheim EAB, et al. A complicated complex: Ion channels, voltage sensing, cell membranes and peptide inhibitors. Neurosci Lett. 2018;679:35-47.
Zhang, A. H., Sharma, G., Undheim, E. A. B., Jia, X., & Mobli, M. (2018). A complicated complex: Ion channels, voltage sensing, cell membranes and peptide inhibitors. Neuroscience Letters, 679, 35-47. https://doi.org/10.1016/j.neulet.2018.04.030
Zhang AH, et al. A Complicated Complex: Ion Channels, Voltage Sensing, Cell Membranes and Peptide Inhibitors. Neurosci Lett. 2018 07 13;679:35-47. PubMed PMID: 29684532.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A complicated complex: Ion channels, voltage sensing, cell membranes and peptide inhibitors. AU - Zhang,Alan H, AU - Sharma,Gagan, AU - Undheim,Eivind A B, AU - Jia,Xinying, AU - Mobli,Mehdi, Y1 - 2018/04/21/ PY - 2018/01/18/received PY - 2018/04/11/revised PY - 2018/04/17/accepted PY - 2018/4/24/pubmed PY - 2019/2/27/medline PY - 2018/4/24/entrez KW - Cell membrane KW - Gating modifier toxins KW - Lipid binding KW - Receptor-ligand complex KW - Venom peptide KW - Voltage-gated ion channel SP - 35 EP - 47 JF - Neuroscience letters JO - Neurosci. Lett. VL - 679 N2 - Voltage-gated ion channels (VGICs) are specialised ion channels that have a voltage dependent mode of action, where ion conduction, or gating, is controlled by a voltage-sensing mechanism. VGICs are critical for electrical signalling and are therefore important pharmacological targets. Among these, voltage-gated sodium channels (NaVs) have attracted particular attention as potential analgesic targets. NaVs, however, comprise several structurally similar subtypes with unique localisations and distinct functions, ranging from amplification of action potentials in nociception (e.g. NaV1.7) to controlling electrical signalling in cardiac function (NaV1.5). Understanding the structural basis of NaV function is therefore of great significance, both to our knowledge of electrical signalling and in development of subtype and state selective drugs. An important tool in this pursuit has been the use of peptides from animal venoms as selective NaV modulators. In this review, we look at peptides, particularly from spider venoms, that inhibit NaVs by binding to the voltage sensing domain (VSD) of this channel, known as gating modifier toxins (GMT). In the first part of the review, we look at the structural determinants of voltage sensing in VGICs, the gating cycle and the conformational changes that accompany VSD movement. Next, the modulation of the analgesic target NaV1.7 by GMTs is reviewed to develop bioinformatic tools that, based on sequence information alone, can identify toxins that are likely to inhibit this channel. The same approach is also used to define VSD sequences, other than that from NaV1.7, which are likely to be sensitive to this class of toxins. The final section of the review focuses on the important role of the cellular membrane in channel modulation and also how the lipid composition affects measurements of peptide-channel interactions both in binding kinetics measurements in solution and in cell-based functional assays. SN - 1872-7972 UR - https://www.unboundmedicine.com/medline/citation/29684532/A_complicated_complex:_Ion_channels_voltage_sensing_cell_membranes_and_peptide_inhibitors_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3940(18)30293-3 DB - PRIME DP - Unbound Medicine ER -