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Trans-cinnamic acid application for rainbow trout (Oncorhynchus mykiss): I. Effects on haematological, serum biochemical, non-specific immune and head kidney gene expression responses.
Fish Shellfish Immunol. 2018 Jul; 78:140-157.FS

Abstract

The present study investigated the effects of dietary trans-cinnamic acid (CA) on pre- and post-challenge haematological, serum biochemical, non-specific immune and head kidney gene expression responses of rainbow trout, Oncorhynchus mykiss juveniles. In this regard, fish with an average weight of 17.01 ± 0.05 g were divided into five groups, and fed daily with an additive free basal diet (control); 250, 500, 750 or 1500 mg kg-1 CA for a 60-day period. Fish were sampled every 20 days during the experiment. On days 20, 40 and 60 (the pre-challenge period), the dietary CA especially at 250 and/or 500 mg kg-1 significantly increased blood granulocyte percentage, and serum total protein, globulin, lysozyme and total immunoglobulin values. Furthermore, dietary CA increased activities of phagocytic activity, respiratory burst and potential killing, and increased the expression levels of immune related genes [serum amyloid A (SAA), interleukin 8 (IL-8), interleukin 1, beta (IL-1β), transforming growth factor beta (TGF-β), tumor necrosis factor (TNF-α), and immunoglobulin T (IgT)] in the head kidney of fish fed with 250 and/or 500 mg kg-1 CA. Following 60 days of feeding, fish were challenged with Yersinia ruckeri and mortality was recorded for 20 days. Highest percentage survival (%) rate was found in the 250 and/or 500 mg kg-1 CA-supplemented feeding groups. During the post-challenge period, red blood cell (RBC) count, hematocrit (%), respiratory burst activity, and total antiprotease activity increased in fish fed with feed containing 500 mg kg-1 content. Moreover, markedly up-regulated the expression of related genes (SAA, IL-8, IL-1β, TGF-β, TNF-α, IFN-γ and IgM) in fish fed 250, 500 and/or 750 mg kg-1 CA. Therefore, feeding O. mykiss for 60 days with dietary CA at 250-500 mg kg-1 CA incorporation levels can be suggested as optimal to enhance the immunity and disease resistance against Y. ruckeri.

Authors+Show Affiliations

Department of Aquaculture, Faculty of Marine Sciences and Technology, Canakkale Onsekiz Mart University, Canakkale 17100, Turkey. Electronic address: sevdanyilmaz@comu.edu.tr.Department of Aquaculture, Faculty of Marine Sciences and Technology, Canakkale Onsekiz Mart University, Canakkale 17100, Turkey.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29684602

Citation

Yılmaz, Sevdan, and Sebahattin Ergün. "Trans-cinnamic Acid Application for Rainbow Trout (Oncorhynchus Mykiss): I. Effects On Haematological, Serum Biochemical, Non-specific Immune and Head Kidney Gene Expression Responses." Fish & Shellfish Immunology, vol. 78, 2018, pp. 140-157.
Yılmaz S, Ergün S. Trans-cinnamic acid application for rainbow trout (Oncorhynchus mykiss): I. Effects on haematological, serum biochemical, non-specific immune and head kidney gene expression responses. Fish Shellfish Immunol. 2018;78:140-157.
Yılmaz, S., & Ergün, S. (2018). Trans-cinnamic acid application for rainbow trout (Oncorhynchus mykiss): I. Effects on haematological, serum biochemical, non-specific immune and head kidney gene expression responses. Fish & Shellfish Immunology, 78, 140-157. https://doi.org/10.1016/j.fsi.2018.04.034
Yılmaz S, Ergün S. Trans-cinnamic Acid Application for Rainbow Trout (Oncorhynchus Mykiss): I. Effects On Haematological, Serum Biochemical, Non-specific Immune and Head Kidney Gene Expression Responses. Fish Shellfish Immunol. 2018;78:140-157. PubMed PMID: 29684602.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Trans-cinnamic acid application for rainbow trout (Oncorhynchus mykiss): I. Effects on haematological, serum biochemical, non-specific immune and head kidney gene expression responses. AU - Yılmaz,Sevdan, AU - Ergün,Sebahattin, Y1 - 2018/04/21/ PY - 2018/01/15/received PY - 2018/04/06/revised PY - 2018/04/18/accepted PY - 2018/4/24/pubmed PY - 2018/9/15/medline PY - 2018/4/24/entrez KW - Cytokine response KW - Innate immunity KW - Rainbow trout KW - Trans-cinnamic acid KW - Yersinia ruckeri SP - 140 EP - 157 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol VL - 78 N2 - The present study investigated the effects of dietary trans-cinnamic acid (CA) on pre- and post-challenge haematological, serum biochemical, non-specific immune and head kidney gene expression responses of rainbow trout, Oncorhynchus mykiss juveniles. In this regard, fish with an average weight of 17.01 ± 0.05 g were divided into five groups, and fed daily with an additive free basal diet (control); 250, 500, 750 or 1500 mg kg-1 CA for a 60-day period. Fish were sampled every 20 days during the experiment. On days 20, 40 and 60 (the pre-challenge period), the dietary CA especially at 250 and/or 500 mg kg-1 significantly increased blood granulocyte percentage, and serum total protein, globulin, lysozyme and total immunoglobulin values. Furthermore, dietary CA increased activities of phagocytic activity, respiratory burst and potential killing, and increased the expression levels of immune related genes [serum amyloid A (SAA), interleukin 8 (IL-8), interleukin 1, beta (IL-1β), transforming growth factor beta (TGF-β), tumor necrosis factor (TNF-α), and immunoglobulin T (IgT)] in the head kidney of fish fed with 250 and/or 500 mg kg-1 CA. Following 60 days of feeding, fish were challenged with Yersinia ruckeri and mortality was recorded for 20 days. Highest percentage survival (%) rate was found in the 250 and/or 500 mg kg-1 CA-supplemented feeding groups. During the post-challenge period, red blood cell (RBC) count, hematocrit (%), respiratory burst activity, and total antiprotease activity increased in fish fed with feed containing 500 mg kg-1 content. Moreover, markedly up-regulated the expression of related genes (SAA, IL-8, IL-1β, TGF-β, TNF-α, IFN-γ and IgM) in fish fed 250, 500 and/or 750 mg kg-1 CA. Therefore, feeding O. mykiss for 60 days with dietary CA at 250-500 mg kg-1 CA incorporation levels can be suggested as optimal to enhance the immunity and disease resistance against Y. ruckeri. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/29684602/Trans_cinnamic_acid_application_for_rainbow_trout__Oncorhynchus_mykiss_:_I__Effects_on_haematological_serum_biochemical_non_specific_immune_and_head_kidney_gene_expression_responses_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1050-4648(18)30219-5 DB - PRIME DP - Unbound Medicine ER -