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Antioxidant and cytotoxic activities of Dendrobium moniliforme extracts and the detection of related compounds by GC-MS.
BMC Complement Altern Med. 2018 Apr 23; 18(1):134.BC

Abstract

BACKGROUND

The medicinal orchid Dendrobium moniliforme contains water-soluble polysaccharides, phenanthrenes, bibenzyl derivatives, and polyphenol compounds. This study explored the antioxidant and cytotoxic activities of D. moniliforme extracts and detected their bioactive compounds.

METHODS

Plant material was collected from the Daman of Makawanpur district in central Nepal. Plant extracts were prepared from stems using hexane, chloroform, acetone, ethanol and methanol. The total polyphenol content (TPC) in each extract was determined using Folin-Ciocalteu's reagent and the total flavonoid content (TFC) in each extract was determined using the aluminium chloride method. The in vitro antioxidant and cytotoxic activities of each extract were determined using DPPH (2,2-diphenyl-1-picrylhydrazyl) and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays respectively. Gas chromatography and mass spectrometry (GC-MS) analysis was used to detect bioactive compounds.

RESULTS

TPC content was highest (116.65 μg GAE/mg of extract) in D. moniliforme chloroform extract (DMC) and TFC content was highest (116.67 μg QE/mg of extract) in D. moniliforme acetone extract (DMA). D. moniliforme hexane extract (DMH) extract showed the highest percentage of DPPH radical scavenging activity (94.48%), followed closely by D. moniliforme ethanol extract (DME) (94.45%), DMA (93.71%) and DMC (94.35%) at 800 μg/ml concentration. The antioxidant capacities of DMC, DMA, DMH and DME, which were measured in IC50 values, were much lower 42.39 μg/ml, 49.56 μg/ml, 52.68 μg/ml, and 58.77 μg/ml respectively than the IC50 of D. moniliforme methanol extract (DMM) (223.15 μg/ml). DMM at the concentration of 800 μg/ml most inhibited the growth of HeLa cells (78.68%) and DME at the same concentration most inhibited the growth of U251 cells (51.95%). The cytotoxic capacity (IC50) of DMM against HeLa cells was 155.80 μg/ml of extract and that of DME against the U251 cells was 772.50 μg/ml of extract. A number of bioactive compounds were detected in both DME and DMM.

CONCLUSION

The fact that plant extract of D. moniliforme has a number of bioactive compounds which showed antioxidant and cytotoxic activities suggests the potential pharmacological importance of this plant.

Authors+Show Affiliations

Central Department of Botany, Tribhuvan University, Kirtipur, Kathmandu, Nepal.Central Department of Botany, Tribhuvan University, Kirtipur, Kathmandu, Nepal.Annapurna Research Center, Kathmandu, Nepal.Central Department of Botany, Tribhuvan University, Kirtipur, Kathmandu, Nepal. bijayapant@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29685150

Citation

Paudel, Mukti Ram, et al. "Antioxidant and Cytotoxic Activities of Dendrobium Moniliforme Extracts and the Detection of Related Compounds By GC-MS." BMC Complementary and Alternative Medicine, vol. 18, no. 1, 2018, p. 134.
Paudel MR, Chand MB, Pant B, et al. Antioxidant and cytotoxic activities of Dendrobium moniliforme extracts and the detection of related compounds by GC-MS. BMC Complement Altern Med. 2018;18(1):134.
Paudel, M. R., Chand, M. B., Pant, B., & Pant, B. (2018). Antioxidant and cytotoxic activities of Dendrobium moniliforme extracts and the detection of related compounds by GC-MS. BMC Complementary and Alternative Medicine, 18(1), 134. https://doi.org/10.1186/s12906-018-2197-6
Paudel MR, et al. Antioxidant and Cytotoxic Activities of Dendrobium Moniliforme Extracts and the Detection of Related Compounds By GC-MS. BMC Complement Altern Med. 2018 Apr 23;18(1):134. PubMed PMID: 29685150.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant and cytotoxic activities of Dendrobium moniliforme extracts and the detection of related compounds by GC-MS. AU - Paudel,Mukti Ram, AU - Chand,Mukesh Babu, AU - Pant,Basant, AU - Pant,Bijaya, Y1 - 2018/04/23/ PY - 2018/02/21/received PY - 2018/04/10/accepted PY - 2018/4/25/entrez PY - 2018/4/25/pubmed PY - 2018/5/19/medline KW - DPPH KW - Dendrobium moniliforme KW - GC-MS KW - HeLa cell line KW - MTT KW - U251 cell line SP - 134 EP - 134 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 18 IS - 1 N2 - BACKGROUND: The medicinal orchid Dendrobium moniliforme contains water-soluble polysaccharides, phenanthrenes, bibenzyl derivatives, and polyphenol compounds. This study explored the antioxidant and cytotoxic activities of D. moniliforme extracts and detected their bioactive compounds. METHODS: Plant material was collected from the Daman of Makawanpur district in central Nepal. Plant extracts were prepared from stems using hexane, chloroform, acetone, ethanol and methanol. The total polyphenol content (TPC) in each extract was determined using Folin-Ciocalteu's reagent and the total flavonoid content (TFC) in each extract was determined using the aluminium chloride method. The in vitro antioxidant and cytotoxic activities of each extract were determined using DPPH (2,2-diphenyl-1-picrylhydrazyl) and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays respectively. Gas chromatography and mass spectrometry (GC-MS) analysis was used to detect bioactive compounds. RESULTS: TPC content was highest (116.65 μg GAE/mg of extract) in D. moniliforme chloroform extract (DMC) and TFC content was highest (116.67 μg QE/mg of extract) in D. moniliforme acetone extract (DMA). D. moniliforme hexane extract (DMH) extract showed the highest percentage of DPPH radical scavenging activity (94.48%), followed closely by D. moniliforme ethanol extract (DME) (94.45%), DMA (93.71%) and DMC (94.35%) at 800 μg/ml concentration. The antioxidant capacities of DMC, DMA, DMH and DME, which were measured in IC50 values, were much lower 42.39 μg/ml, 49.56 μg/ml, 52.68 μg/ml, and 58.77 μg/ml respectively than the IC50 of D. moniliforme methanol extract (DMM) (223.15 μg/ml). DMM at the concentration of 800 μg/ml most inhibited the growth of HeLa cells (78.68%) and DME at the same concentration most inhibited the growth of U251 cells (51.95%). The cytotoxic capacity (IC50) of DMM against HeLa cells was 155.80 μg/ml of extract and that of DME against the U251 cells was 772.50 μg/ml of extract. A number of bioactive compounds were detected in both DME and DMM. CONCLUSION: The fact that plant extract of D. moniliforme has a number of bioactive compounds which showed antioxidant and cytotoxic activities suggests the potential pharmacological importance of this plant. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/29685150/Antioxidant_and_cytotoxic_activities_of_Dendrobium_moniliforme_extracts_and_the_detection_of_related_compounds_by_GC_MS_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-018-2197-6 DB - PRIME DP - Unbound Medicine ER -