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[Advances in application of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 system in stem cells research].
Zhonghua Shao Shang Za Zhi. 2018 Apr 20; 34(4):253-256.ZS

Abstract

Gene engineering has attracted worldwide attention because of its ability of precise location of disease mutations in genome. As a new gene editing technology, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) system is simple, fast, and accurate to operate at a specific gene site. It overcomes the long-standing problem of conventional operation. At the same time, stem cells are a good foundation for establishing disease model in vitro. Therefore, it has great significance to combine stem cells with the rapidly developing gene manipulation techniques. In this review, we mainly focus on the mechanism of CRISPR/Cas9 technology and its application in stem cell genomic editing, so as to pave the way for promoting rapid application and development of CRISPR/Cas9 technology.

Authors+Show Affiliations

College of Life Sciences, General Hospital of PLA, Beijing 100853, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Review

Language

chi

PubMed ID

29690746

Citation

Sun, S J., et al. "[Advances in Application of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated 9 System in Stem Cells Research]." Zhonghua Shao Shang Za Zhi = Zhonghua Shaoshang Zazhi = Chinese Journal of Burns, vol. 34, no. 4, 2018, pp. 253-256.
Sun SJ, Huo JH, Geng ZJ, et al. [Advances in application of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 system in stem cells research]. Zhonghua Shao Shang Za Zhi. 2018;34(4):253-256.
Sun, S. J., Huo, J. H., Geng, Z. J., Sun, X. Y., & Fu, X. B. (2018). [Advances in application of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 system in stem cells research]. Zhonghua Shao Shang Za Zhi = Zhonghua Shaoshang Zazhi = Chinese Journal of Burns, 34(4), 253-256. https://doi.org/10.3760/cma.j.issn.1009-2587.2018.04.013
Sun SJ, et al. [Advances in Application of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated 9 System in Stem Cells Research]. Zhonghua Shao Shang Za Zhi. 2018 Apr 20;34(4):253-256. PubMed PMID: 29690746.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Advances in application of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 system in stem cells research]. AU - Sun,S J, AU - Huo,J H, AU - Geng,Z J, AU - Sun,X Y, AU - Fu,X B, PY - 2018/4/26/entrez PY - 2018/4/25/pubmed PY - 2018/6/29/medline KW - Clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated 9 KW - Gene editing KW - Stem cells SP - 253 EP - 256 JF - Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of burns JO - Zhonghua Shao Shang Za Zhi VL - 34 IS - 4 N2 - Gene engineering has attracted worldwide attention because of its ability of precise location of disease mutations in genome. As a new gene editing technology, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) system is simple, fast, and accurate to operate at a specific gene site. It overcomes the long-standing problem of conventional operation. At the same time, stem cells are a good foundation for establishing disease model in vitro. Therefore, it has great significance to combine stem cells with the rapidly developing gene manipulation techniques. In this review, we mainly focus on the mechanism of CRISPR/Cas9 technology and its application in stem cell genomic editing, so as to pave the way for promoting rapid application and development of CRISPR/Cas9 technology. SN - 1009-2587 UR - https://www.unboundmedicine.com/medline/citation/29690746/[Advances_in_application_of_clustered_regularly_interspaced_short_palindromic_repeats__CRISPR_/CRISPR_associated_9_system_in_stem_cells_research]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&issn=1009-2587&year=2018&vol=34&issue=4&fpage=253 DB - PRIME DP - Unbound Medicine ER -