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Tween-20 transiently changes the surface morphology of PK-15 cells and improves PCV2 infection.
BMC Vet Res. 2018 Apr 24; 14(1):138.BV

Abstract

BACKGROUND

Low concentrations of nonionic surfactants can change the physical properties of cell membranes, and thus and in turn increase drug permeability. Porcine circovirus 2 (PCV2) is an extremely slow-growing virus, and PCV2 infection of PK-15 cells yields very low viral titers. The present study investigates the effect of various nonionic surfactants, namely, Tween-20, Tween-28, Tween-40, Tween-80, Brij-30, Brij-35, NP-40, and Triton X-100 on PCV2 infection and yield in PK-15 cells.

RESULT

Significantly increased PCV2 infection was observed in cells treated with Tween-20 compared to those treated with Tween-28, Tween-40, Brij-30, Brij-35, NP-40, and Triton X-100 (p < 0.01). Furthermore, 24 h incubation with 0.03% Tween-20 has shown to induce significant cellular morphologic changes (cell membrane underwent slight intumescence and bulged into a balloon, and the number of microvilli decreased), as well as to increase caspase-3 activity and to decrease cell viability in PCV2-infected PK-15 cells cmpared to control group; all these changes were restored to normal after Tween-20 has been washed out from the plate.

CONCLUSION

Our data demonstrate that Tween-20 transiently changes the surface morphology of PK-15 cells and improves PCV2 infection. The findings of the present study may be utilized in the development of a PCV2 vaccine.

Authors+Show Affiliations

Institute of Veterinary Immunology & Engineering, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China. National Research Center of Engineering and Technology for Veterinary Biologicals, Jiangsu Academy of Agricultural Science, Nanjing, 210014, China. Key lab of Food Quality and Safety of Jiangsu Province-State Key laboratory Breeding Base, Jiangsu Academy of Agricultural Science, Nanjing, 210014, China. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China.Institute of Veterinary Immunology & Engineering, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China. National Research Center of Engineering and Technology for Veterinary Biologicals, Jiangsu Academy of Agricultural Science, Nanjing, 210014, China. Key lab of Food Quality and Safety of Jiangsu Province-State Key laboratory Breeding Base, Jiangsu Academy of Agricultural Science, Nanjing, 210014, China. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China.Institute of Veterinary Immunology & Engineering, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China. National Research Center of Engineering and Technology for Veterinary Biologicals, Jiangsu Academy of Agricultural Science, Nanjing, 210014, China. Key lab of Food Quality and Safety of Jiangsu Province-State Key laboratory Breeding Base, Jiangsu Academy of Agricultural Science, Nanjing, 210014, China. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China.Institute of Veterinary Immunology & Engineering, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China. National Research Center of Engineering and Technology for Veterinary Biologicals, Jiangsu Academy of Agricultural Science, Nanjing, 210014, China. Key lab of Food Quality and Safety of Jiangsu Province-State Key laboratory Breeding Base, Jiangsu Academy of Agricultural Science, Nanjing, 210014, China. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China.Institute of Veterinary Immunology & Engineering, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China. National Research Center of Engineering and Technology for Veterinary Biologicals, Jiangsu Academy of Agricultural Science, Nanjing, 210014, China. Key lab of Food Quality and Safety of Jiangsu Province-State Key laboratory Breeding Base, Jiangsu Academy of Agricultural Science, Nanjing, 210014, China. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China.Institute of Veterinary Immunology & Engineering, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China. National Research Center of Engineering and Technology for Veterinary Biologicals, Jiangsu Academy of Agricultural Science, Nanjing, 210014, China. Key lab of Food Quality and Safety of Jiangsu Province-State Key laboratory Breeding Base, Jiangsu Academy of Agricultural Science, Nanjing, 210014, China. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China.Institute of Veterinary Immunology & Engineering, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China. National Research Center of Engineering and Technology for Veterinary Biologicals, Jiangsu Academy of Agricultural Science, Nanjing, 210014, China. Key lab of Food Quality and Safety of Jiangsu Province-State Key laboratory Breeding Base, Jiangsu Academy of Agricultural Science, Nanjing, 210014, China. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China.Institute of Veterinary Immunology & Engineering, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China. 782644144@qq.com. National Research Center of Engineering and Technology for Veterinary Biologicals, Jiangsu Academy of Agricultural Science, Nanjing, 210014, China. 782644144@qq.com. Key lab of Food Quality and Safety of Jiangsu Province-State Key laboratory Breeding Base, Jiangsu Academy of Agricultural Science, Nanjing, 210014, China. 782644144@qq.com. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China. 782644144@qq.com.Institute of Veterinary Immunology & Engineering, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China. houjiboccvv@163.com. National Research Center of Engineering and Technology for Veterinary Biologicals, Jiangsu Academy of Agricultural Science, Nanjing, 210014, China. houjiboccvv@163.com. Key lab of Food Quality and Safety of Jiangsu Province-State Key laboratory Breeding Base, Jiangsu Academy of Agricultural Science, Nanjing, 210014, China. houjiboccvv@163.com. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China. houjiboccvv@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29699558

Citation

Hua, Tao, et al. "Tween-20 Transiently Changes the Surface Morphology of PK-15 Cells and Improves PCV2 Infection." BMC Veterinary Research, vol. 14, no. 1, 2018, p. 138.
Hua T, Zhang X, Tang B, et al. Tween-20 transiently changes the surface morphology of PK-15 cells and improves PCV2 infection. BMC Vet Res. 2018;14(1):138.
Hua, T., Zhang, X., Tang, B., Chang, C., Liu, G., Feng, L., Yu, Y., Zhang, D., & Hou, J. (2018). Tween-20 transiently changes the surface morphology of PK-15 cells and improves PCV2 infection. BMC Veterinary Research, 14(1), 138. https://doi.org/10.1186/s12917-018-1457-5
Hua T, et al. Tween-20 Transiently Changes the Surface Morphology of PK-15 Cells and Improves PCV2 Infection. BMC Vet Res. 2018 Apr 24;14(1):138. PubMed PMID: 29699558.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tween-20 transiently changes the surface morphology of PK-15 cells and improves PCV2 infection. AU - Hua,Tao, AU - Zhang,Xuehua, AU - Tang,Bo, AU - Chang,Chen, AU - Liu,Guoyang, AU - Feng,Lei, AU - Yu,Yang, AU - Zhang,Daohua, AU - Hou,Jibo, Y1 - 2018/04/24/ PY - 2017/05/01/received PY - 2018/04/16/accepted PY - 2018/4/28/entrez PY - 2018/4/28/pubmed PY - 2018/8/31/medline KW - Cellular morphologic change KW - Nonionic surfactant KW - PCV2 KW - Tween-20 KW - Viral infection SP - 138 EP - 138 JF - BMC veterinary research JO - BMC Vet. Res. VL - 14 IS - 1 N2 - BACKGROUND: Low concentrations of nonionic surfactants can change the physical properties of cell membranes, and thus and in turn increase drug permeability. Porcine circovirus 2 (PCV2) is an extremely slow-growing virus, and PCV2 infection of PK-15 cells yields very low viral titers. The present study investigates the effect of various nonionic surfactants, namely, Tween-20, Tween-28, Tween-40, Tween-80, Brij-30, Brij-35, NP-40, and Triton X-100 on PCV2 infection and yield in PK-15 cells. RESULT: Significantly increased PCV2 infection was observed in cells treated with Tween-20 compared to those treated with Tween-28, Tween-40, Brij-30, Brij-35, NP-40, and Triton X-100 (p < 0.01). Furthermore, 24 h incubation with 0.03% Tween-20 has shown to induce significant cellular morphologic changes (cell membrane underwent slight intumescence and bulged into a balloon, and the number of microvilli decreased), as well as to increase caspase-3 activity and to decrease cell viability in PCV2-infected PK-15 cells cmpared to control group; all these changes were restored to normal after Tween-20 has been washed out from the plate. CONCLUSION: Our data demonstrate that Tween-20 transiently changes the surface morphology of PK-15 cells and improves PCV2 infection. The findings of the present study may be utilized in the development of a PCV2 vaccine. SN - 1746-6148 UR - https://www.unboundmedicine.com/medline/citation/29699558/Tween-20_transiently_changes_the_surface_morphology_of_PK-15_cells_and_improves_PCV2_infection L2 - https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-018-1457-5 DB - PRIME DP - Unbound Medicine ER -