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Xanthomonas oryzae pv. oryzae TALE proteins recruit OsTFIIAγ1 to compensate for the absence of OsTFIIAγ5 in bacterial blight in rice.
Mol Plant Pathol. 2018 10; 19(10):2248-2262.MP

Abstract

Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of bacterial blight (BB) of rice, uses transcription activator-like effectors (TALEs) to interact with the basal transcription factor gamma subunit OsTFIIAγ5 (Xa5) and activates the transcription of host genes. However, how OsTFIIAγ1, the other OsTFIIAγ protein, functions in the presence of TALEs remains unclear. In this study, we show that OsTFIIAγ1 plays a compensatory role in the absence of Xa5. The expression of OsTFIIAγ1, which is activated by TALE PthXo7, increases the expression of host genes targeted by avirulent and virulent TALEs. Defective OsTFIIAγ1 rice lines show reduced expression of the TALE-targeted susceptibility (S) genes, OsSWEET11 and OsSWEET14, which results in increased BB resistance. Selected TALEs (PthXo1, AvrXa7 and AvrXa27) were evaluated for interactions with OsTFIIAγ1, Xa5 and xa5 (naturally occurring mutant form of Xa5) using biomolecular fluorescence complementation (BiFC) and microscale thermophoresis (MST). BiFC and MST demonstrated that the three TALEs bind Xa5 and OsTFIIAγ1 with a stronger affinity than xa5. These results provide insights into the complex roles of OsTFIIAγ1 and OsTFIIAγ5 in TALE-mediated host gene transcription.

Authors+Show Affiliations

School of Agriculture and Biology, Shanghai Jiao Tong University/Key Laboratory of Urban Agriculture by Ministry of Agriculture, Shanghai, 200240, China. State Key Laboratory of Microbial Metabolism, School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.School of Agriculture and Biology, Shanghai Jiao Tong University/Key Laboratory of Urban Agriculture by Ministry of Agriculture, Shanghai, 200240, China. State Key Laboratory of Microbial Metabolism, School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.School of Agriculture and Biology, Shanghai Jiao Tong University/Key Laboratory of Urban Agriculture by Ministry of Agriculture, Shanghai, 200240, China. State Key Laboratory of Microbial Metabolism, School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.School of Agriculture and Biology, Shanghai Jiao Tong University/Key Laboratory of Urban Agriculture by Ministry of Agriculture, Shanghai, 200240, China. State Key Laboratory of Microbial Metabolism, School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.School of Agriculture and Biology, Shanghai Jiao Tong University/Key Laboratory of Urban Agriculture by Ministry of Agriculture, Shanghai, 200240, China. State Key Laboratory of Microbial Metabolism, School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.School of Agriculture and Biology, Shanghai Jiao Tong University/Key Laboratory of Urban Agriculture by Ministry of Agriculture, Shanghai, 200240, China. State Key Laboratory of Microbial Metabolism, School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.School of Agriculture and Biology, Shanghai Jiao Tong University/Key Laboratory of Urban Agriculture by Ministry of Agriculture, Shanghai, 200240, China. The Center for Plant Science Innovation, University of Nebraska, Lincoln, NE, 68588-0660, USA.School of Agriculture and Biology, Shanghai Jiao Tong University/Key Laboratory of Urban Agriculture by Ministry of Agriculture, Shanghai, 200240, China. State Key Laboratory of Microbial Metabolism, School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29704467

Citation

Ma, Wenxiu, et al. "Xanthomonas Oryzae Pv. Oryzae TALE Proteins Recruit OsTFIIAγ1 to Compensate for the Absence of OsTFIIAγ5 in Bacterial Blight in Rice." Molecular Plant Pathology, vol. 19, no. 10, 2018, pp. 2248-2262.
Ma W, Zou L, Zhiyuan JI, et al. Xanthomonas oryzae pv. oryzae TALE proteins recruit OsTFIIAγ1 to compensate for the absence of OsTFIIAγ5 in bacterial blight in rice. Mol Plant Pathol. 2018;19(10):2248-2262.
Ma, W., Zou, L., Zhiyuan, J. I., Xiameng, X. U., Zhengyin, X. U., Yang, Y., Alfano, J. R., & Chen, G. (2018). Xanthomonas oryzae pv. oryzae TALE proteins recruit OsTFIIAγ1 to compensate for the absence of OsTFIIAγ5 in bacterial blight in rice. Molecular Plant Pathology, 19(10), 2248-2262. https://doi.org/10.1111/mpp.12696
Ma W, et al. Xanthomonas Oryzae Pv. Oryzae TALE Proteins Recruit OsTFIIAγ1 to Compensate for the Absence of OsTFIIAγ5 in Bacterial Blight in Rice. Mol Plant Pathol. 2018;19(10):2248-2262. PubMed PMID: 29704467.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Xanthomonas oryzae pv. oryzae TALE proteins recruit OsTFIIAγ1 to compensate for the absence of OsTFIIAγ5 in bacterial blight in rice. AU - Ma,Wenxiu, AU - Zou,Lifang, AU - Zhiyuan,J I, AU - Xiameng,X U, AU - Zhengyin,X U, AU - Yang,Yangyang, AU - Alfano,James R, AU - Chen,Gongyou, Y1 - 2018/08/07/ PY - 2018/03/19/received PY - 2018/04/24/revised PY - 2018/04/26/accepted PY - 2018/4/29/pubmed PY - 2019/3/19/medline PY - 2018/4/29/entrez KW - Oryza sativa KW - OsTFIIAγ1 KW - Xanthomonas oryzae pv. oryzae KW - bacterial blight KW - susceptibility KW - transcription activator-like effector SP - 2248 EP - 2262 JF - Molecular plant pathology JO - Mol Plant Pathol VL - 19 IS - 10 N2 - Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of bacterial blight (BB) of rice, uses transcription activator-like effectors (TALEs) to interact with the basal transcription factor gamma subunit OsTFIIAγ5 (Xa5) and activates the transcription of host genes. However, how OsTFIIAγ1, the other OsTFIIAγ protein, functions in the presence of TALEs remains unclear. In this study, we show that OsTFIIAγ1 plays a compensatory role in the absence of Xa5. The expression of OsTFIIAγ1, which is activated by TALE PthXo7, increases the expression of host genes targeted by avirulent and virulent TALEs. Defective OsTFIIAγ1 rice lines show reduced expression of the TALE-targeted susceptibility (S) genes, OsSWEET11 and OsSWEET14, which results in increased BB resistance. Selected TALEs (PthXo1, AvrXa7 and AvrXa27) were evaluated for interactions with OsTFIIAγ1, Xa5 and xa5 (naturally occurring mutant form of Xa5) using biomolecular fluorescence complementation (BiFC) and microscale thermophoresis (MST). BiFC and MST demonstrated that the three TALEs bind Xa5 and OsTFIIAγ1 with a stronger affinity than xa5. These results provide insights into the complex roles of OsTFIIAγ1 and OsTFIIAγ5 in TALE-mediated host gene transcription. SN - 1364-3703 UR - https://www.unboundmedicine.com/medline/citation/29704467/Xanthomonas_oryzae_pv__oryzae_TALE_proteins_recruit_OsTFIIAγ1_to_compensate_for_the_absence_of_OsTFIIAγ5_in_bacterial_blight_in_rice_ L2 - https://doi.org/10.1111/mpp.12696 DB - PRIME DP - Unbound Medicine ER -