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Growth, physiological, antioxidants, and immune response of African catfish, Clarias gariepinus (B.), to dietary clove basil, Ocimum gratissimum, leaf extract and its susceptibility to Listeria monocytogenes infection.
Fish Shellfish Immunol. 2018 Jul; 78:346-354.FS

Abstract

Clove basil, Ocimum gratissimum, is a native plant to Africa and grows virtually in tropical and subtropical regions. It has good aroma and its leaves have become used as a spicy and in traditional medicine. The use of plant leaves in fish diets may deteriorate their growth because it may content anti-nutritional factors. Thus, it is better to use plants leaves extract. In the current study, clove basil leaves extract (CBLE) was administrated to African catfish, Clarias gariepinus (B.) to evaluate its effect on growth performance, physiological, antioxidants, and innate immunity variables. Fish (10.7 ± 0.5 g) were fed on diets enriched with 0.0, 5, 10, or 15 g CBLE/kg diet for 12 weeks. After the feeding trial, fish were further exposed to pathogenic bacteria (Listeria monocytogenes) for 14 days. Fish performance and feed intake were significantly enhanced with increasing CBLE levels and its optimum level is found to be 12 g/kg diet. It is noticed that the dietary CBLE in African catfish diets increased significantly the intestinal villi length, villi width, and absorption area in a dose-dependent manner and fish weight was highly correlated with villi length, villi width, and absorption area (R2 = 0.91, 0.91, and 0.92, respectively). On the other side, Dietary CBLE has significant modulatory effect on hemato- and physiological variables of African catfish in a dose-dependent manner. In this regard, blood glucose and cholesterol levels decreased significantly; mean while total protein, albumin, and globulin increased significantly in fish fed high CBLE levels (10-15 g/kg diet). Furthermore, activities of aspartate aminotransferase and alanine aminotransferase, alkaline phosphatase, urea, and creatinine levels were significantly elevated with increasing dietary CBLE levels and their maximum values were detected in fish fed 15 g CBLE/kg diet. Antioxidants and immunity variables were significantly enhanced by CBLE supplementation. Additionally, fish mortality after bacterial challenge was highest in fish fed the control diet (85%) than those fed CBLE-enriched diets. The lowest fish mortality was observed in fish fed 15 g CBLE/kg diet (13.5%). This study evoked that CBLE administration enhanced the performance, feed utilization, antioxidant, and innate immunity properties of African catfish with optimum level of 12 g/kg diet. Also, its supplementation enhanced fish challenge against L. monocytogenes.

Authors+Show Affiliations

Department of Fish Biology and Ecology, Central Laboratory for Aquaculture Research, Abbassa, Abo-Hammad, Sharqia, 44662, Egypt. Electronic address: mohsentawwab@gmail.com.Department of Aquaculture and Fisheries Management, University of Ilorin, Ilorin, Nigeria. Electronic address: adesina.i@unilorin.edu.ng.Department of Aquaculture and Fisheries Management, University of Ilorin, Ilorin, Nigeria. Electronic address: jentolaoni@yahoo.com.Department of Aquaculture and Fisheries Management, University of Ibadan, Ibadan, Nigeria. Electronic address: ekajani@yahoo.co.uk.Department of Veterinary Pathology, University of Ibadan, Ibadan, Nigeria. Electronic address: banabis2001@yahoo.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29704554

Citation

Abdel-Tawwab, Mohsen, et al. "Growth, Physiological, Antioxidants, and Immune Response of African Catfish, Clarias Gariepinus (B.), to Dietary Clove Basil, Ocimum Gratissimum, Leaf Extract and Its Susceptibility to Listeria Monocytogenes Infection." Fish & Shellfish Immunology, vol. 78, 2018, pp. 346-354.
Abdel-Tawwab M, Adeshina I, Jenyo-Oni A, et al. Growth, physiological, antioxidants, and immune response of African catfish, Clarias gariepinus (B.), to dietary clove basil, Ocimum gratissimum, leaf extract and its susceptibility to Listeria monocytogenes infection. Fish Shellfish Immunol. 2018;78:346-354.
Abdel-Tawwab, M., Adeshina, I., Jenyo-Oni, A., Ajani, E. K., & Emikpe, B. O. (2018). Growth, physiological, antioxidants, and immune response of African catfish, Clarias gariepinus (B.), to dietary clove basil, Ocimum gratissimum, leaf extract and its susceptibility to Listeria monocytogenes infection. Fish & Shellfish Immunology, 78, 346-354. https://doi.org/10.1016/j.fsi.2018.04.057
Abdel-Tawwab M, et al. Growth, Physiological, Antioxidants, and Immune Response of African Catfish, Clarias Gariepinus (B.), to Dietary Clove Basil, Ocimum Gratissimum, Leaf Extract and Its Susceptibility to Listeria Monocytogenes Infection. Fish Shellfish Immunol. 2018;78:346-354. PubMed PMID: 29704554.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Growth, physiological, antioxidants, and immune response of African catfish, Clarias gariepinus (B.), to dietary clove basil, Ocimum gratissimum, leaf extract and its susceptibility to Listeria monocytogenes infection. AU - Abdel-Tawwab,Mohsen, AU - Adeshina,Ibrahim, AU - Jenyo-Oni,Adetola, AU - Ajani,Emmanuel K, AU - Emikpe,Benjami O, Y1 - 2018/04/26/ PY - 2017/11/21/received PY - 2018/03/21/revised PY - 2018/04/24/accepted PY - 2018/4/29/pubmed PY - 2018/9/15/medline PY - 2018/4/29/entrez KW - African catfish KW - Antioxidants activity KW - Growth performance KW - Hematology KW - Innate immunity KW - Listeria monocytogenes KW - Ocimum gratissimum KW - Physiological variables KW - Phytobiotic SP - 346 EP - 354 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol VL - 78 N2 - Clove basil, Ocimum gratissimum, is a native plant to Africa and grows virtually in tropical and subtropical regions. It has good aroma and its leaves have become used as a spicy and in traditional medicine. The use of plant leaves in fish diets may deteriorate their growth because it may content anti-nutritional factors. Thus, it is better to use plants leaves extract. In the current study, clove basil leaves extract (CBLE) was administrated to African catfish, Clarias gariepinus (B.) to evaluate its effect on growth performance, physiological, antioxidants, and innate immunity variables. Fish (10.7 ± 0.5 g) were fed on diets enriched with 0.0, 5, 10, or 15 g CBLE/kg diet for 12 weeks. After the feeding trial, fish were further exposed to pathogenic bacteria (Listeria monocytogenes) for 14 days. Fish performance and feed intake were significantly enhanced with increasing CBLE levels and its optimum level is found to be 12 g/kg diet. It is noticed that the dietary CBLE in African catfish diets increased significantly the intestinal villi length, villi width, and absorption area in a dose-dependent manner and fish weight was highly correlated with villi length, villi width, and absorption area (R2 = 0.91, 0.91, and 0.92, respectively). On the other side, Dietary CBLE has significant modulatory effect on hemato- and physiological variables of African catfish in a dose-dependent manner. In this regard, blood glucose and cholesterol levels decreased significantly; mean while total protein, albumin, and globulin increased significantly in fish fed high CBLE levels (10-15 g/kg diet). Furthermore, activities of aspartate aminotransferase and alanine aminotransferase, alkaline phosphatase, urea, and creatinine levels were significantly elevated with increasing dietary CBLE levels and their maximum values were detected in fish fed 15 g CBLE/kg diet. Antioxidants and immunity variables were significantly enhanced by CBLE supplementation. Additionally, fish mortality after bacterial challenge was highest in fish fed the control diet (85%) than those fed CBLE-enriched diets. The lowest fish mortality was observed in fish fed 15 g CBLE/kg diet (13.5%). This study evoked that CBLE administration enhanced the performance, feed utilization, antioxidant, and innate immunity properties of African catfish with optimum level of 12 g/kg diet. Also, its supplementation enhanced fish challenge against L. monocytogenes. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/29704554/Growth_physiological_antioxidants_and_immune_response_of_African_catfish_Clarias_gariepinus__B___to_dietary_clove_basil_Ocimum_gratissimum_leaf_extract_and_its_susceptibility_to_Listeria_monocytogenes_infection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1050-4648(18)30249-3 DB - PRIME DP - Unbound Medicine ER -