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A population of wheat multiple synthetic derivatives: an effective platform to explore, harness and utilize genetic diversity of Aegilops tauschii for wheat improvement.
Theor Appl Genet. 2018 Aug; 131(8):1615-1626.TA

Abstract

KEY MESSAGE

The multiple synthetic derivatives platform described in this study will provide an opportunity for effective utilization of Aegilops tauschii traits and genes for wheat breeding. Introducing genes from wild relatives is the best option to increase genetic diversity and discover new alleles necessary for wheat improvement. A population harboring genomic fragments from the diploid wheat progenitor Aegilops tauschii Coss. in the background of bread wheat (Triticum aestivum L.) was developed by crossing and backcrossing 43 synthetic wheat lines with the common wheat cultivar Norin 61. We named this population multiple synthetic derivatives (MSD). To validate the suitability of this population for wheat breeding and genetic studies, we randomly selected 400 MSD lines and genotyped them by using Diversity Array Technology sequencing markers. We scored black glume as a qualitative trait and heading time in two environments in Sudan as a quantitative trait. Our results showed high genetic diversity and less recombination which is expected from the nature of the population. Genome-wide association (GWA) analysis showed one QTL at the short arm of chromosome 1D different from those alleles reported previously indicating that black glume in the MSD population is controlled by new allele at the same locus. For heading time, from the two environments, GWA analysis revealed three QTLs on the short arms of chromosomes 2A, 2B and 2D and two on the long arms of chromosomes 5A and 5D. Using the MSD population, which represents the diversity of 43 Ae. tauschii accessions representing most of its natural habitat, QTLs or genes and desired phenotypes (such as drought, heat and salinity tolerance) could be identified and selected for utilization in wheat breeding.

Authors+Show Affiliations

Arid Land Research Center, Tottori University, 1390 Hamasaka, Tottori, 680-0001, Japan. Agricultural Research Corporation, P. O. Box: 126, Wad Medani, Sudan.RIKEN Center for Sustainable Resource Science, Tsukuba, Ibaraki, 305-0074, Japan.Agricultural Research Corporation, P. O. Box: 126, Wad Medani, Sudan.Arid Land Research Center, Tottori University, 1390 Hamasaka, Tottori, 680-0001, Japan. tsujim@alrc.tottori-u.ac.jp.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29705916

Citation

Gorafi, Yasir Serag Alnor, et al. "A Population of Wheat Multiple Synthetic Derivatives: an Effective Platform to Explore, Harness and Utilize Genetic Diversity of Aegilops Tauschii for Wheat Improvement." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 131, no. 8, 2018, pp. 1615-1626.
Gorafi YSA, Kim JS, Elbashir AAE, et al. A population of wheat multiple synthetic derivatives: an effective platform to explore, harness and utilize genetic diversity of Aegilops tauschii for wheat improvement. Theor Appl Genet. 2018;131(8):1615-1626.
Gorafi, Y. S. A., Kim, J. S., Elbashir, A. A. E., & Tsujimoto, H. (2018). A population of wheat multiple synthetic derivatives: an effective platform to explore, harness and utilize genetic diversity of Aegilops tauschii for wheat improvement. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 131(8), 1615-1626. https://doi.org/10.1007/s00122-018-3102-x
Gorafi YSA, et al. A Population of Wheat Multiple Synthetic Derivatives: an Effective Platform to Explore, Harness and Utilize Genetic Diversity of Aegilops Tauschii for Wheat Improvement. Theor Appl Genet. 2018;131(8):1615-1626. PubMed PMID: 29705916.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A population of wheat multiple synthetic derivatives: an effective platform to explore, harness and utilize genetic diversity of Aegilops tauschii for wheat improvement. AU - Gorafi,Yasir Serag Alnor, AU - Kim,June-Sik, AU - Elbashir,Awad Ahmed Elawad, AU - Tsujimoto,Hisashi, Y1 - 2018/04/28/ PY - 2018/01/23/received PY - 2018/04/21/accepted PY - 2018/5/1/pubmed PY - 2018/8/23/medline PY - 2018/4/30/entrez SP - 1615 EP - 1626 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 131 IS - 8 N2 - KEY MESSAGE: The multiple synthetic derivatives platform described in this study will provide an opportunity for effective utilization of Aegilops tauschii traits and genes for wheat breeding. Introducing genes from wild relatives is the best option to increase genetic diversity and discover new alleles necessary for wheat improvement. A population harboring genomic fragments from the diploid wheat progenitor Aegilops tauschii Coss. in the background of bread wheat (Triticum aestivum L.) was developed by crossing and backcrossing 43 synthetic wheat lines with the common wheat cultivar Norin 61. We named this population multiple synthetic derivatives (MSD). To validate the suitability of this population for wheat breeding and genetic studies, we randomly selected 400 MSD lines and genotyped them by using Diversity Array Technology sequencing markers. We scored black glume as a qualitative trait and heading time in two environments in Sudan as a quantitative trait. Our results showed high genetic diversity and less recombination which is expected from the nature of the population. Genome-wide association (GWA) analysis showed one QTL at the short arm of chromosome 1D different from those alleles reported previously indicating that black glume in the MSD population is controlled by new allele at the same locus. For heading time, from the two environments, GWA analysis revealed three QTLs on the short arms of chromosomes 2A, 2B and 2D and two on the long arms of chromosomes 5A and 5D. Using the MSD population, which represents the diversity of 43 Ae. tauschii accessions representing most of its natural habitat, QTLs or genes and desired phenotypes (such as drought, heat and salinity tolerance) could be identified and selected for utilization in wheat breeding. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/29705916/A_population_of_wheat_multiple_synthetic_derivatives:_an_effective_platform_to_explore_harness_and_utilize_genetic_diversity_of_Aegilops_tauschii_for_wheat_improvement_ L2 - https://dx.doi.org/10.1007/s00122-018-3102-x DB - PRIME DP - Unbound Medicine ER -