Tags

Type your tag names separated by a space and hit enter

Inhibitory potentials of phenolic-rich extracts from Bridelia ferruginea on two key carbohydrate-metabolizing enzymes and Fe2+-induced pancreatic oxidative stress.
J Integr Med. 2018 05; 16(3):192-198.JI

Abstract

OBJECTIVE

The current study was designed to evaluate the various antioxidant potentials and inhibitory effects of phenolic-rich leaf extracts of Bridelia ferruginea (BF) on the in vitro activities of some key enzymes involved in the metabolism of carbohydrates.

METHODS

In this study, BF leaf free and bound phenolic-rich extracts were used. We quantified total phenolic and flavonoid contents, and evaluated several antioxidant activities using assays for ferric reducing antioxidant power, total antioxidant activity (phosphomolybdenum reducing ability), 1,1-diphenyl-2-picrylhydrazyl and thiobarbituric acid reactive species. Also, extracts were tested for their ability to inhibit α-amylase and α-glucosidase activity.

RESULTS

The total phenolic and total flavonoid contents in the free phenolic extract of BF were significantly greater than in the bound phenolic extract. Also, all the antioxidant activities considered were significantly greater in the free phenolic extract than in the bound phenolic extract. In the same vein, the free phenolic-rich extract had a significantly higher percentage inhibition against α-glucosidase activity (IC50 = 28.5 µg/mL) than the bound phenolic extract (IC50 = 340.0 µg/mL). On the contrary, the free phenolic extract (IC50 = 210.0 µg/mL) had significantly lower inhibition against α-amylase than the bound phenolic-rich extract (IC50 = 190.0 µg/mL).

CONCLUSION

The phenolic-rich extracts of BF leaves showed antioxidant potentials and inhibited two key carbohydrate-metabolizing enzymes in vitro.

Authors+Show Affiliations

Phytomedicine and Toxicology Laboratory, Biochemistry Unit, Department of Chemical Science, College of Science, Afe Babalola University, P.M.B 5454, Ado-Ekiti, Ekiti State, Nigeria. Electronic address: afolabiob@abuad.edu.ng.Department of Biochemistry, Ekiti State University, P.M.B 5363, Ado-Ekiti, Ekiti State, Nigeria.Phytomedicine and Toxicology Laboratory, Biochemistry Unit, Department of Chemical Science, College of Science, Afe Babalola University, P.M.B 5454, Ado-Ekiti, Ekiti State, Nigeria.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29706572

Citation

Afolabi, Olakunle Bamikole, et al. "Inhibitory Potentials of Phenolic-rich Extracts From Bridelia Ferruginea On Two Key Carbohydrate-metabolizing Enzymes and Fe2+-induced Pancreatic Oxidative Stress." Journal of Integrative Medicine, vol. 16, no. 3, 2018, pp. 192-198.
Afolabi OB, Oloyede OI, Agunbiade SO. Inhibitory potentials of phenolic-rich extracts from Bridelia ferruginea on two key carbohydrate-metabolizing enzymes and Fe2+-induced pancreatic oxidative stress. J Integr Med. 2018;16(3):192-198.
Afolabi, O. B., Oloyede, O. I., & Agunbiade, S. O. (2018). Inhibitory potentials of phenolic-rich extracts from Bridelia ferruginea on two key carbohydrate-metabolizing enzymes and Fe2+-induced pancreatic oxidative stress. Journal of Integrative Medicine, 16(3), 192-198. https://doi.org/10.1016/j.joim.2018.04.006
Afolabi OB, Oloyede OI, Agunbiade SO. Inhibitory Potentials of Phenolic-rich Extracts From Bridelia Ferruginea On Two Key Carbohydrate-metabolizing Enzymes and Fe2+-induced Pancreatic Oxidative Stress. J Integr Med. 2018;16(3):192-198. PubMed PMID: 29706572.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibitory potentials of phenolic-rich extracts from Bridelia ferruginea on two key carbohydrate-metabolizing enzymes and Fe2+-induced pancreatic oxidative stress. AU - Afolabi,Olakunle Bamikole, AU - Oloyede,Omotade Ibidun, AU - Agunbiade,Shadrack Oludare, Y1 - 2018/04/18/ PY - 2017/12/27/received PY - 2018/03/15/accepted PY - 2018/5/1/pubmed PY - 2018/10/27/medline PY - 2018/5/1/entrez KW - Bridelia ferruginea KW - Diabetes mellitus KW - Polyphenolic acids KW - α-Amylase KW - α-Glucosidase SP - 192 EP - 198 JF - Journal of integrative medicine JO - J Integr Med VL - 16 IS - 3 N2 - OBJECTIVE: The current study was designed to evaluate the various antioxidant potentials and inhibitory effects of phenolic-rich leaf extracts of Bridelia ferruginea (BF) on the in vitro activities of some key enzymes involved in the metabolism of carbohydrates. METHODS: In this study, BF leaf free and bound phenolic-rich extracts were used. We quantified total phenolic and flavonoid contents, and evaluated several antioxidant activities using assays for ferric reducing antioxidant power, total antioxidant activity (phosphomolybdenum reducing ability), 1,1-diphenyl-2-picrylhydrazyl and thiobarbituric acid reactive species. Also, extracts were tested for their ability to inhibit α-amylase and α-glucosidase activity. RESULTS: The total phenolic and total flavonoid contents in the free phenolic extract of BF were significantly greater than in the bound phenolic extract. Also, all the antioxidant activities considered were significantly greater in the free phenolic extract than in the bound phenolic extract. In the same vein, the free phenolic-rich extract had a significantly higher percentage inhibition against α-glucosidase activity (IC50 = 28.5 µg/mL) than the bound phenolic extract (IC50 = 340.0 µg/mL). On the contrary, the free phenolic extract (IC50 = 210.0 µg/mL) had significantly lower inhibition against α-amylase than the bound phenolic-rich extract (IC50 = 190.0 µg/mL). CONCLUSION: The phenolic-rich extracts of BF leaves showed antioxidant potentials and inhibited two key carbohydrate-metabolizing enzymes in vitro. SN - 2095-4964 UR - https://www.unboundmedicine.com/medline/citation/29706572/Inhibitory_potentials_of_phenolic_rich_extracts_from_Bridelia_ferruginea_on_two_key_carbohydrate_metabolizing_enzymes_and_Fe2+_induced_pancreatic_oxidative_stress_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S2095-4964(18)30045-1 DB - PRIME DP - Unbound Medicine ER -