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Effects of moderate intensity static magnetic fields on osteoclastic differentiation in mouse bone marrow cells.
Bioelectromagnetics. 2018 Jul; 39(5):394-404.B

Abstract

Although we recently demonstrated that static magnetic fields (SMFs) of 3, 15, and 50 mT stimulate osteoblastic differentiation, the effects of SMFs on osteoclastogenesis are still poorly understood. This study focused on the suppressive effects of SMFs on receptor activator of nuclear factor κB ligand (RANKL)-induced osteoclastogenesis and bone resorption. Direct SMFs inhibit RANKL-induced multinucleated osteoclast formation, tartrate-resistant acid phosphatase activity, and bone resorption in mouse bone marrow-derived macrophage cells. The conditioned medium from osteoblasts treated with SMFs also resulted in the inhibition of osteoclast differentiation as well as resorption. The RANKL-induced expression of osteoclast-specific transcription factors, such as c-Fos and NFATc1, was remarkably downregulated by SMF at 15 mT. In addition, SMF inhibited RANKL-activated Akt, glycogen synthase kinase 3β (GSK3β), extracellular signal-regulated kinase, c-jun N-terminal protein kinase, mitogen-activated protein kinase (MAPK), and nuclear factor-κB (NF-κB) formation. These findings indicate that SMF-mediated attenuation of RANKL-induced Akt, GSK3β, MAPK, and NF-κB pathways could contribute to the direct and indirect inhibition of osteoclast formation and bone resorption. Therefore, SMFs could be developed as a therapeutic agent against periprosthetic or peri-implant osteolysis. Additionally, these could be used against osteolytic diseases such as osteoporosis and rheumatoid arthritis. Bioelectromagnetics. 39:394-404, 2018. © 2018 Wiley Periodicals, Inc.

Authors+Show Affiliations

Department of Oral and Maxillofacial Pathology, School of Dentistry, Kyung Hee University, Seoul, South Korea.Department of Oral and Maxillofacial Pathology, School of Dentistry, Kyung Hee University, Seoul, South Korea.School of Mechanical Engineering, Kyungpook National University, Daegu, South Korea.Department of Prosthodontics, School of Dentistry, Kyung Hee University, Seoul, South Korea.Department of Prosthodontics, School of Dentistry, Kyung Hee University, Seoul, South Korea.Department of Dental Materials, School of Dentistry, Kyung Hee University, Seoul, South Korea.Department of Prosthodontics, School of Dentistry, Kyung Hee University, Seoul, South Korea.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29709064

Citation

Kim, Eun-Cheol, et al. "Effects of Moderate Intensity Static Magnetic Fields On Osteoclastic Differentiation in Mouse Bone Marrow Cells." Bioelectromagnetics, vol. 39, no. 5, 2018, pp. 394-404.
Kim EC, Park J, Noh G, et al. Effects of moderate intensity static magnetic fields on osteoclastic differentiation in mouse bone marrow cells. Bioelectromagnetics. 2018;39(5):394-404.
Kim, E. C., Park, J., Noh, G., Park, S. J., Noh, K., Kwon, I. K., & Ahn, S. J. (2018). Effects of moderate intensity static magnetic fields on osteoclastic differentiation in mouse bone marrow cells. Bioelectromagnetics, 39(5), 394-404. https://doi.org/10.1002/bem.22126
Kim EC, et al. Effects of Moderate Intensity Static Magnetic Fields On Osteoclastic Differentiation in Mouse Bone Marrow Cells. Bioelectromagnetics. 2018;39(5):394-404. PubMed PMID: 29709064.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of moderate intensity static magnetic fields on osteoclastic differentiation in mouse bone marrow cells. AU - Kim,Eun-Cheol, AU - Park,Jaesuh, AU - Noh,Gunwoo, AU - Park,Su-Jung, AU - Noh,Kwantae, AU - Kwon,Il-Keun, AU - Ahn,Su-Jin, Y1 - 2018/04/30/ PY - 2017/05/14/received PY - 2018/02/26/accepted PY - 2018/5/1/pubmed PY - 2018/10/3/medline PY - 2018/5/1/entrez KW - RANKL KW - differentiation KW - osteoclast KW - peri-implant KW - resorption SP - 394 EP - 404 JF - Bioelectromagnetics JO - Bioelectromagnetics VL - 39 IS - 5 N2 - Although we recently demonstrated that static magnetic fields (SMFs) of 3, 15, and 50 mT stimulate osteoblastic differentiation, the effects of SMFs on osteoclastogenesis are still poorly understood. This study focused on the suppressive effects of SMFs on receptor activator of nuclear factor κB ligand (RANKL)-induced osteoclastogenesis and bone resorption. Direct SMFs inhibit RANKL-induced multinucleated osteoclast formation, tartrate-resistant acid phosphatase activity, and bone resorption in mouse bone marrow-derived macrophage cells. The conditioned medium from osteoblasts treated with SMFs also resulted in the inhibition of osteoclast differentiation as well as resorption. The RANKL-induced expression of osteoclast-specific transcription factors, such as c-Fos and NFATc1, was remarkably downregulated by SMF at 15 mT. In addition, SMF inhibited RANKL-activated Akt, glycogen synthase kinase 3β (GSK3β), extracellular signal-regulated kinase, c-jun N-terminal protein kinase, mitogen-activated protein kinase (MAPK), and nuclear factor-κB (NF-κB) formation. These findings indicate that SMF-mediated attenuation of RANKL-induced Akt, GSK3β, MAPK, and NF-κB pathways could contribute to the direct and indirect inhibition of osteoclast formation and bone resorption. Therefore, SMFs could be developed as a therapeutic agent against periprosthetic or peri-implant osteolysis. Additionally, these could be used against osteolytic diseases such as osteoporosis and rheumatoid arthritis. Bioelectromagnetics. 39:394-404, 2018. © 2018 Wiley Periodicals, Inc. SN - 1521-186X UR - https://www.unboundmedicine.com/medline/citation/29709064/Effects_of_moderate_intensity_static_magnetic_fields_on_osteoclastic_differentiation_in_mouse_bone_marrow_cells_ L2 - https://doi.org/10.1002/bem.22126 DB - PRIME DP - Unbound Medicine ER -