A cascade autocatalytic strand displacement amplification and hybridization chain reaction event for label-free and ultrasensitive electrochemical nucleic acid biosensing.
Biosens Bioelectron. 2018 Aug 15; 113:1-8.BB

Abstract

Herein, an autocatalytic strand displacement amplification (ASDA) strategy was proposed for the first time, which was further ingeniously coupled with hybridization chain reaction (HCR) event for the isothermal, label-free and multiple amplification toward nucleic acid detection. During the ASDA module, the target recognition opens the immobilized hairpin probe (IP) and initiates the annealing of the auxiliary DNA strand (AS) with the opened IP for the successive polymerization and nicking reaction in the presence of DNA polymerase and nicking endonuclease. This induces the target recycling and generation of a large amount of intermediate DNA sequences, which can be used as target analogy to execute the autocatalytic strand displacement amplification. Simultaneously, the introduced AS strand can propagate the HCR between two hairpins (H1 and H2) to form a linear DNA concatamer with cytosine (C)-rich loop region, which can facilitate the in-situ synthesis of silver nanoclusters (AgNCs) as electrochemical tags for further amplification toward target responses. With current cascade ASDA and HCR strategy, the detection of target DNA could be achieved with a low detection limit of about 0.16 fM and a good selectivity. The developed biosensor also exhibits the distinct advantages of flexibility and simplicity in probe design and biosensor fabrication, and label-free electrochemical detection, thus opens a promising avenue for the detection of nucleic acid with low abundance in bioanalysis and clinical biomedicine.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Chen Z

Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, No. 53, Rd. Zhengzhou, Qingdao, Shandong 266042, China.

MeSH

Biosensing TechniquesDNAElectrochemical TechniquesImmobilized Nucleic AcidsLimit of DetectionMetal NanoparticlesNucleic Acid Amplification TechniquesNucleic Acid HybridizationSilver

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29709776

Citation

Chen, Zhiqiang, et al. "A Cascade Autocatalytic Strand Displacement Amplification and Hybridization Chain Reaction Event for Label-free and Ultrasensitive Electrochemical Nucleic Acid Biosensing." Biosensors & Bioelectronics, vol. 113, 2018, pp. 1-8.

Chen Z, Liu Y, Xin C, et al. A cascade autocatalytic strand displacement amplification and hybridization chain reaction event for label-free and ultrasensitive electrochemical nucleic acid biosensing. Biosens Bioelectron. 2018;113:1-8.

Chen, Z., Liu, Y., Xin, C., Zhao, J., & Liu, S. (2018). A cascade autocatalytic strand displacement amplification and hybridization chain reaction event for label-free and ultrasensitive electrochemical nucleic acid biosensing. Biosensors & Bioelectronics, 113, 1-8. https://doi.org/10.1016/j.bios.2018.04.046

Chen Z, et al. A Cascade Autocatalytic Strand Displacement Amplification and Hybridization Chain Reaction Event for Label-free and Ultrasensitive Electrochemical Nucleic Acid Biosensing. Biosens Bioelectron. 2018 Aug 15;113:1-8. PubMed PMID: 29709776.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - A cascade autocatalytic strand displacement amplification and hybridization chain reaction event for label-free and ultrasensitive electrochemical nucleic acid biosensing. AU - Chen,Zhiqiang, AU - Liu,Ying, AU - Xin,Chen, AU - Zhao,Jikuan, AU - Liu,Shufeng, Y1 - 2018/04/23/ PY - 2018/02/19/received PY - 2018/04/03/revised PY - 2018/04/18/accepted PY - 2018/5/2/pubmed PY - 2018/10/3/medline PY - 2018/5/1/entrez KW - Electrochemical detection KW - Hybridization chain reaction KW - Nucleic acid biosensor KW - Silver nanocluster KW - Strand displacement amplification SP - 1 EP - 8 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 113 N2 - Herein, an autocatalytic strand displacement amplification (ASDA) strategy was proposed for the first time, which was further ingeniously coupled with hybridization chain reaction (HCR) event for the isothermal, label-free and multiple amplification toward nucleic acid detection. During the ASDA module, the target recognition opens the immobilized hairpin probe (IP) and initiates the annealing of the auxiliary DNA strand (AS) with the opened IP for the successive polymerization and nicking reaction in the presence of DNA polymerase and nicking endonuclease. This induces the target recycling and generation of a large amount of intermediate DNA sequences, which can be used as target analogy to execute the autocatalytic strand displacement amplification. Simultaneously, the introduced AS strand can propagate the HCR between two hairpins (H1 and H2) to form a linear DNA concatamer with cytosine (C)-rich loop region, which can facilitate the in-situ synthesis of silver nanoclusters (AgNCs) as electrochemical tags for further amplification toward target responses. With current cascade ASDA and HCR strategy, the detection of target DNA could be achieved with a low detection limit of about 0.16 fM and a good selectivity. The developed biosensor also exhibits the distinct advantages of flexibility and simplicity in probe design and biosensor fabrication, and label-free electrochemical detection, thus opens a promising avenue for the detection of nucleic acid with low abundance in bioanalysis and clinical biomedicine. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/29709776/A_cascade_autocatalytic_strand_displacement_amplification_and_hybridization_chain_reaction_event_for_label_free_and_ultrasensitive_electrochemical_nucleic_acid_biosensing_ DB - PRIME DP - Unbound Medicine ER -

Tags

A cascade autocatalytic strand displacement amplification and hybridization chain reaction event for label-free and ultrasensitive electrochemical nucleic acid biosensing.Biosens Bioelectron. 2018 Aug 15; 113:1-8.BB