Citation
Kim, Kyu Min, et al. "Distinct Gating Mechanism of SOC Channel Involving STIM-Orai Coupling and an Intramolecular Interaction of Orai in Caenorhabditis Elegans." Proceedings of the National Academy of Sciences of the United States of America, vol. 115, no. 20, 2018, pp. E4623-E4632.
Kim KM, Wijerathne T, Hur JH, et al. Distinct gating mechanism of SOC channel involving STIM-Orai coupling and an intramolecular interaction of Orai in Caenorhabditis elegans. Proc Natl Acad Sci USA. 2018;115(20):E4623-E4632.
Kim, K. M., Wijerathne, T., Hur, J. H., Kang, U. J., Kim, I. H., Kweon, Y. C., Lee, A. R., Jeong, S. J., Lee, S. K., Lee, Y. Y., Sim, B. W., Lee, J. H., Baig, C., Kim, S. U., Chang, K. T., Lee, K. P., & Park, C. Y. (2018). Distinct gating mechanism of SOC channel involving STIM-Orai coupling and an intramolecular interaction of Orai in Caenorhabditis elegans. Proceedings of the National Academy of Sciences of the United States of America, 115(20), E4623-E4632. https://doi.org/10.1073/pnas.1714986115
Kim KM, et al. Distinct Gating Mechanism of SOC Channel Involving STIM-Orai Coupling and an Intramolecular Interaction of Orai in Caenorhabditis Elegans. Proc Natl Acad Sci USA. 2018 05 15;115(20):E4623-E4632. PubMed PMID: 29712850.
TY - JOUR
T1 - Distinct gating mechanism of SOC channel involving STIM-Orai coupling and an intramolecular interaction of Orai in Caenorhabditis elegans.
AU - Kim,Kyu Min,
AU - Wijerathne,Tharaka,
AU - Hur,Jin-Hoe,
AU - Kang,Uk Jung,
AU - Kim,Ihn Hyeong,
AU - Kweon,Yeong Cheon,
AU - Lee,Ah Reum,
AU - Jeong,Su Ji,
AU - Lee,Sang Kwon,
AU - Lee,Yoon Young,
AU - Sim,Bo-Woong,
AU - Lee,Jong-Hee,
AU - Baig,Chunggi,
AU - Kim,Sun-Uk,
AU - Chang,Kyu-Tae,
AU - Lee,Kyu Pil,
AU - Park,Chan Young,
Y1 - 2018/04/30/
PY - 2018/5/2/pubmed
PY - 2018/8/29/medline
PY - 2018/5/2/entrez
KW - Ca2+ signaling
KW - Caenorhabditis elegans
KW - Orai1
KW - SOCE
KW - STIM1
SP - E4623
EP - E4632
JF - Proceedings of the National Academy of Sciences of the United States of America
JO - Proc. Natl. Acad. Sci. U.S.A.
VL - 115
IS - 20
N2 - Store-operated calcium entry (SOCE), an important mechanism of Ca2+ signaling in a wide range of cell types, is mediated by stromal interaction molecule (STIM), which senses the depletion of endoplasmic reticulum Ca2+ stores and binds and activates Orai channels in the plasma membrane. This inside-out mechanism of Ca2+ signaling raises an interesting question about the evolution of SOCE: How did these two proteins existing in different cellular compartments evolve to interact with each other? We investigated the gating mechanism of Caenorhabditis elegans Orai channels. Our analysis revealed a mechanism of Orai gating by STIM binding to the intracellular 2-3 loop of Orai in C. elegans that is radically different from Orai gating by STIM binding to the N and C termini of Orai in mammals. In addition, we found that the conserved hydrophobic amino acids in the 2-3 loop of Orai1 are important for the oligomerization and gating of channels and are regulated via an intramolecular interaction mechanism mediated by the N and C termini of Orai1. This study identifies a previously unknown SOCE mechanism in C. elegans and suggests that, while the STIM-Orai interaction is conserved between invertebrates and mammals, the gating mechanism for Orai channels differs considerably.
SN - 1091-6490
UR - https://www.unboundmedicine.com/medline/citation/29712850/Distinct_gating_mechanism_of_SOC_channel_involving_STIM_Orai_coupling_and_an_intramolecular_interaction_of_Orai_in_Caenorhabditis_elegans_
L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=29712850
DB - PRIME
DP - Unbound Medicine
ER -