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Sensitive determination of dopamine levels via surface-enhanced Raman scattering of Ag nanoparticle dimers.
Int J Nanomedicine. 2018; 13:2337-2347.IJ

Abstract

BACKGROUND

Dopamine (DA) is an important neurotransmitter in the hypothalamus and pituitary gland, which can produce a direct influence on mammals' emotions in midbrain. Additionally, the level of DA is highly related with some important neurologic diseases such as schizophrenia, Parkinson, and Huntington's diseases, etc. In light of the important roles that DA plays in the disease modulation, it is of considerable significance to develop a sensitive and reproducible approach for monitoring DA.

PURPOSE

The objective of this study was to develop an efficient approach to quantitatively monitor the level of DA using Ag nanoparticle (NP) dimers and enhanced Raman spectroscopy.

METHODS

Ag NP dimers were synthesized for the sensitive detection of DA via surface-enhanced Raman scattering (SERS). Citrate was used as both the capping agent of NPs and sensing agent to DA, which is self-assembled on the surface of Ag NP dimers by reacting with the surface carboxyl group to form a stable amide bond. To improve accuracy and precision, the multiplicative effects model for surface-enhanced Raman spectroscopy was utilized to analyze the SERS assays.

RESULTS

A low limits of detection (LOD) of 20 pM and a wide linear response range from 30 pM to 300 nM were obtained for DA quantitative detection. The SERS enhancement factor was theoretically valued at approximately 107 by discrete dipole approximation. DA was self-assembled on the citrate capped surface of Ag NPs dimers through the amide bond. The adsorption energy was estimated to be 256 KJ/mol using the Langmuir isotherm model. The density functional theory was used to simulate the spectral characteristics of SERS during the adsorption of DA on the surface of the Ag dimers. Furthermore, to improve the accuracy and precision of quantitative analysis of SERS assays with a multiplicative effects model for surface-enhanced Raman spectroscopy.

CONCLUSION

A LOD of 20 pM DA-level was obtained, and the linear response ranged from 30 pM to 300 nM for quantitative DA detection. The absolute relative percentage error was 4.22% between the real and predicted DA concentrations. This detection scheme is expected to have good applications in the prevention and diagnosis of certain diseases caused by disorders in the DA level.

Authors+Show Affiliations

State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai, China.State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai, China.State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai, China.State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai, China.State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai, China.State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai, China. Collaborative Innovation Center of Extreme Optics, Shanxi University, Taiyuan, Shanxi, China.State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai, China.State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai, China. Collaborative Innovation Center of Extreme Optics, Shanxi University, Taiyuan, Shanxi, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29713165

Citation

Yu, Xiantong, et al. "Sensitive Determination of Dopamine Levels Via Surface-enhanced Raman Scattering of Ag Nanoparticle Dimers." International Journal of Nanomedicine, vol. 13, 2018, pp. 2337-2347.
Yu X, He X, Yang T, et al. Sensitive determination of dopamine levels via surface-enhanced Raman scattering of Ag nanoparticle dimers. Int J Nanomedicine. 2018;13:2337-2347.
Yu, X., He, X., Yang, T., Zhao, L., Chen, Q., Zhang, S., Chen, J., & Xu, J. (2018). Sensitive determination of dopamine levels via surface-enhanced Raman scattering of Ag nanoparticle dimers. International Journal of Nanomedicine, 13, 2337-2347. https://doi.org/10.2147/IJN.S156932
Yu X, et al. Sensitive Determination of Dopamine Levels Via Surface-enhanced Raman Scattering of Ag Nanoparticle Dimers. Int J Nanomedicine. 2018;13:2337-2347. PubMed PMID: 29713165.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sensitive determination of dopamine levels via surface-enhanced Raman scattering of Ag nanoparticle dimers. AU - Yu,Xiantong, AU - He,XiaoXiao, AU - Yang,Taiqun, AU - Zhao,Litao, AU - Chen,Qichen, AU - Zhang,Sanjun, AU - Chen,Jinquan, AU - Xu,Jianhua, Y1 - 2018/04/17/ PY - 2018/5/2/entrez PY - 2018/5/2/pubmed PY - 2018/6/12/medline KW - Ag NP dimers KW - MEMSERS KW - SERS KW - dopamine detection KW - multiplicative effects model for surface-enhanced Raman spectroscopy KW - surface-enhanced Raman scattering SP - 2337 EP - 2347 JF - International journal of nanomedicine JO - Int J Nanomedicine VL - 13 N2 - BACKGROUND: Dopamine (DA) is an important neurotransmitter in the hypothalamus and pituitary gland, which can produce a direct influence on mammals' emotions in midbrain. Additionally, the level of DA is highly related with some important neurologic diseases such as schizophrenia, Parkinson, and Huntington's diseases, etc. In light of the important roles that DA plays in the disease modulation, it is of considerable significance to develop a sensitive and reproducible approach for monitoring DA. PURPOSE: The objective of this study was to develop an efficient approach to quantitatively monitor the level of DA using Ag nanoparticle (NP) dimers and enhanced Raman spectroscopy. METHODS: Ag NP dimers were synthesized for the sensitive detection of DA via surface-enhanced Raman scattering (SERS). Citrate was used as both the capping agent of NPs and sensing agent to DA, which is self-assembled on the surface of Ag NP dimers by reacting with the surface carboxyl group to form a stable amide bond. To improve accuracy and precision, the multiplicative effects model for surface-enhanced Raman spectroscopy was utilized to analyze the SERS assays. RESULTS: A low limits of detection (LOD) of 20 pM and a wide linear response range from 30 pM to 300 nM were obtained for DA quantitative detection. The SERS enhancement factor was theoretically valued at approximately 107 by discrete dipole approximation. DA was self-assembled on the citrate capped surface of Ag NPs dimers through the amide bond. The adsorption energy was estimated to be 256 KJ/mol using the Langmuir isotherm model. The density functional theory was used to simulate the spectral characteristics of SERS during the adsorption of DA on the surface of the Ag dimers. Furthermore, to improve the accuracy and precision of quantitative analysis of SERS assays with a multiplicative effects model for surface-enhanced Raman spectroscopy. CONCLUSION: A LOD of 20 pM DA-level was obtained, and the linear response ranged from 30 pM to 300 nM for quantitative DA detection. The absolute relative percentage error was 4.22% between the real and predicted DA concentrations. This detection scheme is expected to have good applications in the prevention and diagnosis of certain diseases caused by disorders in the DA level. SN - 1178-2013 UR - https://www.unboundmedicine.com/medline/citation/29713165/Sensitive_determination_of_dopamine_levels_via_surface_enhanced_Raman_scattering_of_Ag_nanoparticle_dimers_ L2 - https://dx.doi.org/10.2147/IJN.S156932 DB - PRIME DP - Unbound Medicine ER -