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Oxidation-reduction potential and sperm DNA fragmentation, and their associations with sperm morphological anomalies amongst fertile and infertile men.
Arab J Urol 2018; 16(1):87-95AJ

Abstract

Objective

To assess seminal oxidation-reduction potential (ORP) and sperm DNA fragmentation (SDF) in male infertility and their relationships with sperm morphology in fertile and infertile men.

Patients and methods

Prospective case-control study comparing the findings of infertile men (n = 1168) to those of men with confirmed fertility (n = 100) regarding demographics and semen characteristics (conventional and advanced semen tests). Spearman rank correlation assessed the correlation between ORP, SDF, and different morphological indices. Means of ORP and SDF were assessed in variable levels of normal sperm morphology amongst all participants.

Results

Infertile patients had a significantly lower mean sperm count (32.7 vs 58.7 × 106 sperm/mL), total motility (50.1% vs 60.4%), and normal morphology (5.7% vs 9.9%). Conversely, infertile patients had significantly higher mean head defects (54% vs 48%), and higher ORP and SDF values than fertile controls. ORP and SDF showed significant positive correlations and significant negative correlations with sperm head defects and normal morphology in infertile patients, respectively. ORP and SDF were significantly inversely associated with the level of normal sperm morphology. Using receiver operating characteristic curve analysis, ORP and SDF threshold values of 1.73 mV/106 sperm/mL and 25.5%, respectively, were associated with 76% and 56% sensitivity and 72% and 72.2% specificity, respectively, in differentiating <4% from ≥4% normal morphology.

Conclusion

A direct inverse relationship exists between seminal ORP and SDF with various levels of normal sperm morphology. Using ORP and SDF measures in conjunction with standard semen morphology analysis could validate the result of the fertility status of patients.

Authors+Show Affiliations

Department of Urology, Hamad Medical Corporation, Doha, Qatar.Department of Urology, Hamad Medical Corporation, Doha, Qatar.Department of Urology, Hamad Medical Corporation, Doha, Qatar.Department of Urology, Cleveland Clinic Foundation, Cleveland, USA.Department of Urology, Hamad Medical Corporation, Doha, Qatar.Department of Urology, Hamad Medical Corporation, Doha, Qatar.Department of Surgery, Hamad General Hospital, Hamad Medical Corporation, Doha, Qatar. College of Medicine, Qatar University, Doha, Qatar. School of Health and Education, University of Skövde, Skövde, Sweden.Department of Obstetrics and Gynecology, Women's Hospital, Hamad Medical Corporation, Doha, Qatar.Department of Urology, Hamad Medical Corporation, Doha, Qatar.Department of Urology, Hamad Medical Corporation, Doha, Qatar.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29713539

Citation

Majzoub, Ahmad, et al. "Oxidation-reduction Potential and Sperm DNA Fragmentation, and Their Associations With Sperm Morphological Anomalies Amongst Fertile and Infertile Men." Arab Journal of Urology, vol. 16, no. 1, 2018, pp. 87-95.
Majzoub A, Arafa M, Mahdi M, et al. Oxidation-reduction potential and sperm DNA fragmentation, and their associations with sperm morphological anomalies amongst fertile and infertile men. Arab J Urol. 2018;16(1):87-95.
Majzoub, A., Arafa, M., Mahdi, M., Agarwal, A., Al Said, S., Al-Emadi, I., ... Elbardisi, H. (2018). Oxidation-reduction potential and sperm DNA fragmentation, and their associations with sperm morphological anomalies amongst fertile and infertile men. Arab Journal of Urology, 16(1), pp. 87-95. doi:10.1016/j.aju.2017.11.014.
Majzoub A, et al. Oxidation-reduction Potential and Sperm DNA Fragmentation, and Their Associations With Sperm Morphological Anomalies Amongst Fertile and Infertile Men. Arab J Urol. 2018;16(1):87-95. PubMed PMID: 29713539.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oxidation-reduction potential and sperm DNA fragmentation, and their associations with sperm morphological anomalies amongst fertile and infertile men. AU - Majzoub,Ahmad, AU - Arafa,Mohamad, AU - Mahdi,Mohamed, AU - Agarwal,Ashok, AU - Al Said,Sami, AU - Al-Emadi,Ibrahim, AU - El Ansari,Walid, AU - Alattar,Alia, AU - Al Rumaihi,Khalid, AU - Elbardisi,Haitham, Y1 - 2018/02/01/ PY - 2017/09/14/received PY - 2017/11/11/revised PY - 2017/11/21/accepted PY - 2018/5/2/entrez PY - 2018/5/2/pubmed PY - 2018/5/2/medline KW - ART, assisted reproductive techniques KW - AUC, area under the curve KW - ICSI, intracytoplasmic sperm injection KW - IUI, intrauterine insemination KW - IVF, in vitro fertilisation KW - Male infertility KW - NPV, negative predictive value KW - ORP, oxidation–reduction potential KW - OS, oxidative stress KW - Oxidation-reduction potential KW - PPV, positive predictive value KW - ROC, receiver operating characteristic KW - ROS, reactive oxygen species KW - SCD, sperm chromatin dispersion KW - Sperm KW - Sperm DNA fragmentation KW - Sperm morphology SP - 87 EP - 95 JF - Arab journal of urology JO - Arab J Urol VL - 16 IS - 1 N2 - Objective: To assess seminal oxidation-reduction potential (ORP) and sperm DNA fragmentation (SDF) in male infertility and their relationships with sperm morphology in fertile and infertile men. Patients and methods: Prospective case-control study comparing the findings of infertile men (n = 1168) to those of men with confirmed fertility (n = 100) regarding demographics and semen characteristics (conventional and advanced semen tests). Spearman rank correlation assessed the correlation between ORP, SDF, and different morphological indices. Means of ORP and SDF were assessed in variable levels of normal sperm morphology amongst all participants. Results: Infertile patients had a significantly lower mean sperm count (32.7 vs 58.7 × 106 sperm/mL), total motility (50.1% vs 60.4%), and normal morphology (5.7% vs 9.9%). Conversely, infertile patients had significantly higher mean head defects (54% vs 48%), and higher ORP and SDF values than fertile controls. ORP and SDF showed significant positive correlations and significant negative correlations with sperm head defects and normal morphology in infertile patients, respectively. ORP and SDF were significantly inversely associated with the level of normal sperm morphology. Using receiver operating characteristic curve analysis, ORP and SDF threshold values of 1.73 mV/106 sperm/mL and 25.5%, respectively, were associated with 76% and 56% sensitivity and 72% and 72.2% specificity, respectively, in differentiating <4% from ≥4% normal morphology. Conclusion: A direct inverse relationship exists between seminal ORP and SDF with various levels of normal sperm morphology. Using ORP and SDF measures in conjunction with standard semen morphology analysis could validate the result of the fertility status of patients. SN - 2090-598X UR - https://www.unboundmedicine.com/medline/citation/29713539/Oxidation_reduction_potential_and_sperm_DNA_fragmentation_and_their_associations_with_sperm_morphological_anomalies_amongst_fertile_and_infertile_men_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S2090-598X(17)30148-1 DB - PRIME DP - Unbound Medicine ER -