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[Sperm chromatin structure assay versus sperm chromatin dispersion test in detecting sperm DNA integrity and correlation of sperm DNA fragmentation with semen parameters].
Zhonghua Nan Ke Xue 2017; 23(4):329-336ZN

Abstract

Objective

Sperm DNA fragmentation (SDF) is widely used to predict male infertility and the methods of detecting SDF are varied. This study aimed to compare two methods of SDF detection and investigate the correlation between SDF and sperm quality.

METHODS

Using sperm chromatin structure assay (SCSA) and sperm chromatin dispersion test (SCD), we detected SDF in 108 semen samples collected in the Center of Reproduction and Genetics of Suzhou Municipal Hospital. We compared the results of the two methods and analyzed the correlations of SDF routine semen parameters, sperm morphology and the age of the patients.

RESULTS

A significant consistency was found in the SDF index (DFI) between the two methods (P<0.01). The DFI was correlated negatively with sperm motility, the percentage of progressively motile sperm, and that of morphologically normal sperm (P <0.01), but positively with the teratozoospermia index (P <0.01 in SCSA and P <0.05 in SCD). The DFI measured by SCSA showed a significantly positive correlation with the patients' age (P <0.01), but not that obtained by SCD.

CONCLUSIONS

The results of both SCSA and SCD play an important role in predicting sperm quality. As a clinical index, the DFI has a predictive value for male infertility. However, the results of different detecting methods vary widely, which calls for further studies on their standardization.

Authors+Show Affiliations

Center of Reproduction and Genetics, Suzhou Municipal Hospital Affiliated to Nanjing Medical University, Suzhou, Jiangsu 215002, China.Center of Reproduction and Genetics, Suzhou Municipal Hospital Affiliated to Nanjing Medical University, Suzhou, Jiangsu 215002, China.Center of Reproduction and Genetics, Suzhou Municipal Hospital Affiliated to Nanjing Medical University, Suzhou, Jiangsu 215002, China.Center of Reproduction and Genetics, Suzhou Municipal Hospital Affiliated to Nanjing Medical University, Suzhou, Jiangsu 215002, China.Center of Reproduction and Genetics, Suzhou Municipal Hospital Affiliated to Nanjing Medical University, Suzhou, Jiangsu 215002, China.Center of Reproduction and Genetics, Suzhou Municipal Hospital Affiliated to Nanjing Medical University, Suzhou, Jiangsu 215002, China.Center of Reproduction and Genetics, Suzhou Municipal Hospital Affiliated to Nanjing Medical University, Suzhou, Jiangsu 215002, China.Center of Reproduction and Genetics, Suzhou Municipal Hospital Affiliated to Nanjing Medical University, Suzhou, Jiangsu 215002, China.Center of Reproduction and Genetics, Suzhou Municipal Hospital Affiliated to Nanjing Medical University, Suzhou, Jiangsu 215002, China.Center of Reproduction and Genetics, Suzhou Municipal Hospital Affiliated to Nanjing Medical University, Suzhou, Jiangsu 215002, China.

Pub Type(s)

Comparative Study
Journal Article

Language

chi

PubMed ID

29714418

Citation

Wang, Jia-Xiong, et al. "[Sperm Chromatin Structure Assay Versus Sperm Chromatin Dispersion Test in Detecting Sperm DNA Integrity and Correlation of Sperm DNA Fragmentation With Semen Parameters]." Zhonghua Nan Ke Xue = National Journal of Andrology, vol. 23, no. 4, 2017, pp. 329-336.
Wang JX, Han MT, Shen LY, et al. [Sperm chromatin structure assay versus sperm chromatin dispersion test in detecting sperm DNA integrity and correlation of sperm DNA fragmentation with semen parameters]. Zhonghua Nan Ke Xue. 2017;23(4):329-336.
Wang, J. X., Han, M. T., Shen, L. Y., Wang, G. G., Cheng, H. B., Yang, S. M., ... Shi, Y. C. (2017). [Sperm chromatin structure assay versus sperm chromatin dispersion test in detecting sperm DNA integrity and correlation of sperm DNA fragmentation with semen parameters]. Zhonghua Nan Ke Xue = National Journal of Andrology, 23(4), pp. 329-336.
Wang JX, et al. [Sperm Chromatin Structure Assay Versus Sperm Chromatin Dispersion Test in Detecting Sperm DNA Integrity and Correlation of Sperm DNA Fragmentation With Semen Parameters]. Zhonghua Nan Ke Xue. 2017;23(4):329-336. PubMed PMID: 29714418.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Sperm chromatin structure assay versus sperm chromatin dispersion test in detecting sperm DNA integrity and correlation of sperm DNA fragmentation with semen parameters]. AU - Wang,Jia-Xiong, AU - Han,Mu-Tian, AU - Shen,Li-Yan, AU - Wang,Gai-Gai, AU - Cheng,Hong-Bo, AU - Yang,Shen-Min, AU - Song,Dan, AU - Wang,Wei, AU - Li,Hong, AU - Shi,Yi-Chao, PY - 2018/5/2/entrez PY - 2018/5/2/pubmed PY - 2018/9/15/medline KW - KW - sperm chromatin structure assay KW - age KW - sperm DNA fragmentation KW - sperm chromatin dispersion test KW - sperm motility; percentage of morphologically normal sperm SP - 329 EP - 336 JF - Zhonghua nan ke xue = National journal of andrology JO - Zhonghua Nan Ke Xue VL - 23 IS - 4 N2 - Objective: Sperm DNA fragmentation (SDF) is widely used to predict male infertility and the methods of detecting SDF are varied. This study aimed to compare two methods of SDF detection and investigate the correlation between SDF and sperm quality. METHODS: Using sperm chromatin structure assay (SCSA) and sperm chromatin dispersion test (SCD), we detected SDF in 108 semen samples collected in the Center of Reproduction and Genetics of Suzhou Municipal Hospital. We compared the results of the two methods and analyzed the correlations of SDF routine semen parameters, sperm morphology and the age of the patients. RESULTS: A significant consistency was found in the SDF index (DFI) between the two methods (P<0.01). The DFI was correlated negatively with sperm motility, the percentage of progressively motile sperm, and that of morphologically normal sperm (P <0.01), but positively with the teratozoospermia index (P <0.01 in SCSA and P <0.05 in SCD). The DFI measured by SCSA showed a significantly positive correlation with the patients' age (P <0.01), but not that obtained by SCD. CONCLUSIONS: The results of both SCSA and SCD play an important role in predicting sperm quality. As a clinical index, the DFI has a predictive value for male infertility. However, the results of different detecting methods vary widely, which calls for further studies on their standardization. SN - 1009-3591 UR - https://www.unboundmedicine.com/medline/citation/29714418/[Sperm_chromatin_structure_assay_versus_sperm_chromatin_dispersion_test_in_detecting_sperm_DNA_integrity_and_correlation_of_sperm_DNA_fragmentation_with_semen_parameters]_ L2 - https://medlineplus.gov/maleinfertility.html DB - PRIME DP - Unbound Medicine ER -