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Sequence investigation of 34 forensic autosomal STRs with massively parallel sequencing.
Sci Rep. 2018 05 01; 8(1):6810.SR

Abstract

STRs vary not only in the length of the repeat units and the number of repeats but also in the region with which they conform to an incremental repeat pattern. Massively parallel sequencing (MPS) offers new possibilities in the analysis of STRs since they can simultaneously sequence multiple targets in a single reaction and capture potential internal sequence variations. Here, we sequenced 34 STRs applied in the forensic community of China with a custom-designed panel. MPS performance were evaluated from sequencing reads analysis, concordance study and sensitivity testing. High coverage sequencing data were obtained to determine the constitute ratios and heterozygous balance. No actual inconsistent genotypes were observed between capillary electrophoresis (CE) and MPS, demonstrating the reliability of the panel and the MPS technology. With the sequencing data from the 200 investigated individuals, 346 and 418 alleles were obtained via CE and MPS technologies at the 34 STRs, indicating MPS technology provides higher discrimination than CE detection. The whole study demonstrated that STR genotyping with the custom panel and MPS technology has the potential not only to reveal length and sequence variations but also to satisfy the demands of high throughput and high multiplexing with acceptable sensitivity.

Authors+Show Affiliations

Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Sciences, Ministry of Justice, Shanghai, 200063, P.R. China.Criminal Investigation Department, Ministry of Public Security, Beijing, 100741, P.R. China.Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Sciences, Ministry of Justice, Shanghai, 200063, P.R. China.The Affiliated Guangji Hospital of Soochow University, Suzhou, 215008, P.R. China.Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Sciences, Ministry of Justice, Shanghai, 200063, P.R. China.Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Sciences, Ministry of Justice, Shanghai, 200063, P.R. China.Shanghai OE Biotechnology Co, Ltd, Shanghai, 201114, P.R. China.Department of Forensic Medicine, Medical College of Soochow University, Suzhou, 215123, P.R. China.Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Sciences, Ministry of Justice, Shanghai, 200063, P.R. China. lichengtaohla@163.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29717145

Citation

Zhang, Suhua, et al. "Sequence Investigation of 34 Forensic Autosomal STRs With Massively Parallel Sequencing." Scientific Reports, vol. 8, no. 1, 2018, p. 6810.
Zhang S, Niu Y, Bian Y, et al. Sequence investigation of 34 forensic autosomal STRs with massively parallel sequencing. Sci Rep. 2018;8(1):6810.
Zhang, S., Niu, Y., Bian, Y., Dong, R., Liu, X., Bao, Y., Jin, C., Zheng, H., & Li, C. (2018). Sequence investigation of 34 forensic autosomal STRs with massively parallel sequencing. Scientific Reports, 8(1), 6810. https://doi.org/10.1038/s41598-018-24495-9
Zhang S, et al. Sequence Investigation of 34 Forensic Autosomal STRs With Massively Parallel Sequencing. Sci Rep. 2018 05 1;8(1):6810. PubMed PMID: 29717145.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sequence investigation of 34 forensic autosomal STRs with massively parallel sequencing. AU - Zhang,Suhua, AU - Niu,Yong, AU - Bian,Yingnan, AU - Dong,Rixia, AU - Liu,Xiling, AU - Bao,Yun, AU - Jin,Chao, AU - Zheng,Hancheng, AU - Li,Chengtao, Y1 - 2018/05/01/ PY - 2017/10/27/received PY - 2018/04/05/accepted PY - 2018/5/3/entrez PY - 2018/5/3/pubmed PY - 2019/10/8/medline SP - 6810 EP - 6810 JF - Scientific reports JO - Sci Rep VL - 8 IS - 1 N2 - STRs vary not only in the length of the repeat units and the number of repeats but also in the region with which they conform to an incremental repeat pattern. Massively parallel sequencing (MPS) offers new possibilities in the analysis of STRs since they can simultaneously sequence multiple targets in a single reaction and capture potential internal sequence variations. Here, we sequenced 34 STRs applied in the forensic community of China with a custom-designed panel. MPS performance were evaluated from sequencing reads analysis, concordance study and sensitivity testing. High coverage sequencing data were obtained to determine the constitute ratios and heterozygous balance. No actual inconsistent genotypes were observed between capillary electrophoresis (CE) and MPS, demonstrating the reliability of the panel and the MPS technology. With the sequencing data from the 200 investigated individuals, 346 and 418 alleles were obtained via CE and MPS technologies at the 34 STRs, indicating MPS technology provides higher discrimination than CE detection. The whole study demonstrated that STR genotyping with the custom panel and MPS technology has the potential not only to reveal length and sequence variations but also to satisfy the demands of high throughput and high multiplexing with acceptable sensitivity. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/29717145/Sequence_investigation_of_34_forensic_autosomal_STRs_with_massively_parallel_sequencing_ L2 - https://doi.org/10.1038/s41598-018-24495-9 DB - PRIME DP - Unbound Medicine ER -