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Large-Volume Crystalloid Fluid Is Associated with Increased Hyaluronan Shedding and Inflammation in a Canine Hemorrhagic Shock Model.
Inflammation 2018; 41(4):1515-1523I

Abstract

Shedding of the endothelial glycocalyx precedes leukocyte activation and adherence in acute inflammation. Rapid administration of crystalloid or colloid fluids for treating hemorrhagic shock may cause endothelial glycocalyx shedding, thereby increasing inflammation. This study aimed to compare the effect of different fluid treatments in a canine shock model on glycocalyx biomarker, hyaluronan, and inflammatory biomarkers. Greyhound dogs under general anesthesia subject to hemorrhage for 60 min were given 20 mL kg-1 of either fresh whole blood (FWB), hydroxyethyl starch (HES) 130/0.4, 4% succinylated gelatin (GELO), or 80 mL kg-1 of isotonic crystalloid (CRYST) over 20 min (n = 6 per group). Plasma biomarkers hyaluronan, interleukin (IL) 6, 8, 10, tumor necrosis factor-α, monocyte chemoattractant protein-1, keratinocyte chemokine-like, and atrial natriuretic peptide were measured at baseline, end of hemorrhage (Shock), end of fluid administration (T20), and then 40 (T60), 100 (T120), and 160 (T180) minutes later. Biomarker concentrations were compared between groups using the Kruskal-Wallis test or Fisher's exact test (measurable versus unmeasurable) (significance set at P < 0.05). Hyaluronan concentration peaked early in the CRYST group at T20, compared to HES (P = 0.005) and GELO (P = 0.018), and later in the GELO group at T60, compared to FWB (P < 0.001). The CRYST group had significantly more samples with measurable IL6 at T180 (P = 0.015), compared to GELO, and IL10 at T60, T120, and T180 (all P = 0.015), compared to FWB. There were no significant differences in other biomarker concentrations. In conclusion, rapid large-volume crystalloid administered for hemorrhagic shock was associated with increased hyaluronan and a greater inflammatory response.

Authors+Show Affiliations

Comparative Health Research Group, School of Veterinary and Life Sciences, Murdoch University, 90 South St, Murdoch, Perth, WA, 6163, Australia. l.smart@murdoch.edu.au. Centre for Clinical Research in Emergency Medicine, Harry Perkins Institute of Medical Research, Perth, Australia. l.smart@murdoch.edu.au. Emergency Medicine, University of Western Australia, Perth, Australia. l.smart@murdoch.edu.au.Comparative Health Research Group, School of Veterinary and Life Sciences, Murdoch University, 90 South St, Murdoch, Perth, WA, 6163, Australia.Comparative Health Research Group, School of Veterinary and Life Sciences, Murdoch University, 90 South St, Murdoch, Perth, WA, 6163, Australia.Centre for Clinical Research in Emergency Medicine, Harry Perkins Institute of Medical Research, Perth, Australia. Emergency Medicine, University of Western Australia, Perth, Australia.Comparative Health Research Group, School of Veterinary and Life Sciences, Murdoch University, 90 South St, Murdoch, Perth, WA, 6163, Australia.Comparative Health Research Group, School of Veterinary and Life Sciences, Murdoch University, 90 South St, Murdoch, Perth, WA, 6163, Australia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29728805

Citation

Smart, Lisa, et al. "Large-Volume Crystalloid Fluid Is Associated With Increased Hyaluronan Shedding and Inflammation in a Canine Hemorrhagic Shock Model." Inflammation, vol. 41, no. 4, 2018, pp. 1515-1523.
Smart L, Boyd CJ, Claus MA, et al. Large-Volume Crystalloid Fluid Is Associated with Increased Hyaluronan Shedding and Inflammation in a Canine Hemorrhagic Shock Model. Inflammation. 2018;41(4):1515-1523.
Smart, L., Boyd, C. J., Claus, M. A., Bosio, E., Hosgood, G., & Raisis, A. (2018). Large-Volume Crystalloid Fluid Is Associated with Increased Hyaluronan Shedding and Inflammation in a Canine Hemorrhagic Shock Model. Inflammation, 41(4), pp. 1515-1523. doi:10.1007/s10753-018-0797-4.
Smart L, et al. Large-Volume Crystalloid Fluid Is Associated With Increased Hyaluronan Shedding and Inflammation in a Canine Hemorrhagic Shock Model. Inflammation. 2018;41(4):1515-1523. PubMed PMID: 29728805.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Large-Volume Crystalloid Fluid Is Associated with Increased Hyaluronan Shedding and Inflammation in a Canine Hemorrhagic Shock Model. AU - Smart,Lisa, AU - Boyd,C J, AU - Claus,M A, AU - Bosio,E, AU - Hosgood,G, AU - Raisis,A, PY - 2018/5/8/pubmed PY - 2018/11/27/medline PY - 2018/5/6/entrez KW - cytokine KW - endothelium KW - fluid resuscitation KW - shock SP - 1515 EP - 1523 JF - Inflammation JO - Inflammation VL - 41 IS - 4 N2 - Shedding of the endothelial glycocalyx precedes leukocyte activation and adherence in acute inflammation. Rapid administration of crystalloid or colloid fluids for treating hemorrhagic shock may cause endothelial glycocalyx shedding, thereby increasing inflammation. This study aimed to compare the effect of different fluid treatments in a canine shock model on glycocalyx biomarker, hyaluronan, and inflammatory biomarkers. Greyhound dogs under general anesthesia subject to hemorrhage for 60 min were given 20 mL kg-1 of either fresh whole blood (FWB), hydroxyethyl starch (HES) 130/0.4, 4% succinylated gelatin (GELO), or 80 mL kg-1 of isotonic crystalloid (CRYST) over 20 min (n = 6 per group). Plasma biomarkers hyaluronan, interleukin (IL) 6, 8, 10, tumor necrosis factor-α, monocyte chemoattractant protein-1, keratinocyte chemokine-like, and atrial natriuretic peptide were measured at baseline, end of hemorrhage (Shock), end of fluid administration (T20), and then 40 (T60), 100 (T120), and 160 (T180) minutes later. Biomarker concentrations were compared between groups using the Kruskal-Wallis test or Fisher's exact test (measurable versus unmeasurable) (significance set at P < 0.05). Hyaluronan concentration peaked early in the CRYST group at T20, compared to HES (P = 0.005) and GELO (P = 0.018), and later in the GELO group at T60, compared to FWB (P < 0.001). The CRYST group had significantly more samples with measurable IL6 at T180 (P = 0.015), compared to GELO, and IL10 at T60, T120, and T180 (all P = 0.015), compared to FWB. There were no significant differences in other biomarker concentrations. In conclusion, rapid large-volume crystalloid administered for hemorrhagic shock was associated with increased hyaluronan and a greater inflammatory response. SN - 1573-2576 UR - https://www.unboundmedicine.com/medline/citation/29728805/Large_Volume_Crystalloid_Fluid_Is_Associated_with_Increased_Hyaluronan_Shedding_and_Inflammation_in_a_Canine_Hemorrhagic_Shock_Model_ L2 - https://doi.org/10.1007/s10753-018-0797-4 DB - PRIME DP - Unbound Medicine ER -