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Nanozyme-based lateral flow assay for the sensitive detection of Escherichia coli O157:H7 in milk.
J Dairy Sci. 2018 Jul; 101(7):5770-5779.JD

Abstract

Lateral flow assay (LFA) has been applied in many fields due to its relative ease of use and cost-effectiveness. However, it has low sensitivity and its applications are limited. Probe materials play a significant role in improving the detection efficiency and sensitivity of LFA. In this study, by using concave palladium-platinum (Pd-Pt) nanoparticles as a nanozyme probe, we developed a sensitive LFA based on the sandwich format for qualitative and quantitative detection of Escherichia coli O157:H7. The sensitivity of the LFA was improved by applying the 3,3',5,5'-tetramethylbenzidine (TMB) substrate onto the test line where the nanozyme was accumulated in the presence of analytes. The nanozyme showed high catalytic performance toward TMB and greatly enhanced the signal intensity of the test line. The sensitivity of the nanozyme-based LFA was 9.0 × 102 cfu/mL in milk, which was 111-fold higher than that of traditional colloidal gold-based LFA. The proposed method has remarkable potential in the detection of various pathogens in real samples.

Authors+Show Affiliations

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; Key Laboratory of Marine Materials and Related Technologies, Zhejiang Key Laboratory of Marine Materials and Protective Technologies, Ningbo Institute of Material Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, China.Key Laboratory of Marine Materials and Related Technologies, Zhejiang Key Laboratory of Marine Materials and Protective Technologies, Ningbo Institute of Material Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, China.State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.Key Laboratory of Marine Materials and Related Technologies, Zhejiang Key Laboratory of Marine Materials and Protective Technologies, Ningbo Institute of Material Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, China.Key Laboratory of Marine Materials and Related Technologies, Zhejiang Key Laboratory of Marine Materials and Protective Technologies, Ningbo Institute of Material Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, China; University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China; Max Planck Institute for Polymer Research, Ackermannweg 10, 55128, Mainz, Germany. Electronic address: yjhuang@nimte.ac.cn.Key Laboratory of Marine Materials and Related Technologies, Zhejiang Key Laboratory of Marine Materials and Protective Technologies, Ningbo Institute of Material Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, China.State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. Electronic address: talktolaiwh@163.com.Key Laboratory of Marine Materials and Related Technologies, Zhejiang Key Laboratory of Marine Materials and Protective Technologies, Ningbo Institute of Material Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, China. Electronic address: tao.chen@nimte.ac.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29729911

Citation

Han, Jiaojiao, et al. "Nanozyme-based Lateral Flow Assay for the Sensitive Detection of Escherichia Coli O157:H7 in Milk." Journal of Dairy Science, vol. 101, no. 7, 2018, pp. 5770-5779.
Han J, Zhang L, Hu L, et al. Nanozyme-based lateral flow assay for the sensitive detection of Escherichia coli O157:H7 in milk. J Dairy Sci. 2018;101(7):5770-5779.
Han, J., Zhang, L., Hu, L., Xing, K., Lu, X., Huang, Y., Zhang, J., Lai, W., & Chen, T. (2018). Nanozyme-based lateral flow assay for the sensitive detection of Escherichia coli O157:H7 in milk. Journal of Dairy Science, 101(7), 5770-5779. https://doi.org/10.3168/jds.2018-14429
Han J, et al. Nanozyme-based Lateral Flow Assay for the Sensitive Detection of Escherichia Coli O157:H7 in Milk. J Dairy Sci. 2018;101(7):5770-5779. PubMed PMID: 29729911.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nanozyme-based lateral flow assay for the sensitive detection of Escherichia coli O157:H7 in milk. AU - Han,Jiaojiao, AU - Zhang,Lei, AU - Hu,Liming, AU - Xing,Keyu, AU - Lu,Xuefei, AU - Huang,Youju, AU - Zhang,Jiawei, AU - Lai,Weihua, AU - Chen,Tao, Y1 - 2018/05/03/ PY - 2018/01/11/received PY - 2018/03/18/accepted PY - 2018/5/8/pubmed PY - 2019/3/6/medline PY - 2018/5/7/entrez KW - 3,3′,5,5′-tetramethylbenzidine KW - Escherichia coli O157:H7 KW - lateral flow assay KW - nanozyme SP - 5770 EP - 5779 JF - Journal of dairy science JO - J. Dairy Sci. VL - 101 IS - 7 N2 - Lateral flow assay (LFA) has been applied in many fields due to its relative ease of use and cost-effectiveness. However, it has low sensitivity and its applications are limited. Probe materials play a significant role in improving the detection efficiency and sensitivity of LFA. In this study, by using concave palladium-platinum (Pd-Pt) nanoparticles as a nanozyme probe, we developed a sensitive LFA based on the sandwich format for qualitative and quantitative detection of Escherichia coli O157:H7. The sensitivity of the LFA was improved by applying the 3,3',5,5'-tetramethylbenzidine (TMB) substrate onto the test line where the nanozyme was accumulated in the presence of analytes. The nanozyme showed high catalytic performance toward TMB and greatly enhanced the signal intensity of the test line. The sensitivity of the nanozyme-based LFA was 9.0 × 102 cfu/mL in milk, which was 111-fold higher than that of traditional colloidal gold-based LFA. The proposed method has remarkable potential in the detection of various pathogens in real samples. SN - 1525-3198 UR - https://www.unboundmedicine.com/medline/citation/29729911/Nanozyme_based_lateral_flow_assay_for_the_sensitive_detection_of_Escherichia_coli_O157:H7_in_milk_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-0302(18)30411-9 DB - PRIME DP - Unbound Medicine ER -