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Microwave-assisted extraction for Hibiscus sabdariffa bioactive compounds.
J Pharm Biomed Anal. 2018 Jul 15; 156:313-322.JP

Abstract

H. sabdariffa has demonstrated positive results against chronic diseases due to the presence of phytochemicals, mainly phenolic compounds. The extraction process of bioactive compounds increases the efficient collection of extracts with high bioactivity. Microwave-Assisted Extraction (MAE) constituted a "green technology" widely employed for plant matrix. In this work, the impact of temperature (50-150 °C), composition of extraction solvent (15-75% EtOH) and extraction time (5-20 min) on the extraction yield and individual compounds concentrations were evaluated. Furthermore, the characterization of 16 extracts obtained was performed by HPLC-ESI-TOF-MS. The results showed that 164 °C, 12.5 min, 45% ethanol was the best extraction condition, although glycoside flavonoids were degraded. Besides that, the optimal conditions for extraction yield were 164 °C, 60% ethanol and 22 min. Thus, temperature and solvent concentration have demonstrated to be potential factors in MAE for obtaining bioactive compounds from H. sabdariffa.

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Authors+Show Affiliations

Pimentel-Moral S
Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avenida Fuentenueva s/n, 18071 Granada, Spain; Research and Development Functional Food Centre (CIDAF), Health Science Technological Park, Avenida del Conocimiento 37, Edificio BioRegión, 18016 Granada, Spain. Electronic address: spimentel@ugr.es.
Borrás-Linares I
Research and Development Functional Food Centre (CIDAF), Health Science Technological Park, Avenida del Conocimiento 37, Edificio BioRegión, 18016 Granada, Spain. Electronic address: iborras@cidaf.es.
Lozano-Sánchez J
Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avenida Fuentenueva s/n, 18071 Granada, Spain; Research and Development Functional Food Centre (CIDAF), Health Science Technological Park, Avenida del Conocimiento 37, Edificio BioRegión, 18016 Granada, Spain. Electronic address: jesusls@ugr.es.
Arráez-Román D
Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avenida Fuentenueva s/n, 18071 Granada, Spain; Research and Development Functional Food Centre (CIDAF), Health Science Technological Park, Avenida del Conocimiento 37, Edificio BioRegión, 18016 Granada, Spain. Electronic address: darraez@ugr.es.
Martínez-Férez A
Chemical Engineering Department, University of Granada, Avenida Fuentenueva s/n, 18071 Granada, Spain. Electronic address: amferez@ugr.es.
Segura-Carretero A
Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avenida Fuentenueva s/n, 18071 Granada, Spain; Research and Development Functional Food Centre (CIDAF), Health Science Technological Park, Avenida del Conocimiento 37, Edificio BioRegión, 18016 Granada, Spain. Electronic address: ansegura@ugr.es.

MeSH

Chemical FractionationChromatography, High Pressure LiquidEthanolFlavonoidsGreen Chemistry TechnologyHibiscusMass SpectrometryMicrowavesPhenolsPhytochemicalsPlant ExtractsSolventsTemperatureTime Factors

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29734100

Citation

Pimentel-Moral, Sandra, et al. "Microwave-assisted Extraction for Hibiscus Sabdariffa Bioactive Compounds." Journal of Pharmaceutical and Biomedical Analysis, vol. 156, 2018, pp. 313-322.
Pimentel-Moral S, Borrás-Linares I, Lozano-Sánchez J, et al. Microwave-assisted extraction for Hibiscus sabdariffa bioactive compounds. J Pharm Biomed Anal. 2018;156:313-322.
Pimentel-Moral, S., Borrás-Linares, I., Lozano-Sánchez, J., Arráez-Román, D., Martínez-Férez, A., & Segura-Carretero, A. (2018). Microwave-assisted extraction for Hibiscus sabdariffa bioactive compounds. Journal of Pharmaceutical and Biomedical Analysis, 156, 313-322. https://doi.org/10.1016/j.jpba.2018.04.050
Pimentel-Moral S, et al. Microwave-assisted Extraction for Hibiscus Sabdariffa Bioactive Compounds. J Pharm Biomed Anal. 2018 Jul 15;156:313-322. PubMed PMID: 29734100.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Microwave-assisted extraction for Hibiscus sabdariffa bioactive compounds. AU - Pimentel-Moral,Sandra, AU - Borrás-Linares,Isabel, AU - Lozano-Sánchez,Jesús, AU - Arráez-Román,David, AU - Martínez-Férez,Antonio, AU - Segura-Carretero,Antonio, Y1 - 2018/05/01/ PY - 2018/02/12/received PY - 2018/04/26/revised PY - 2018/04/27/accepted PY - 2018/5/8/pubmed PY - 2018/10/23/medline PY - 2018/5/8/entrez KW - Extraction yield KW - HPLC-ESI-TOF-MS KW - Hibiscus sabdariffa KW - Microwave-assisted extraction KW - Phenolic compounds SP - 313 EP - 322 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 156 N2 - H. sabdariffa has demonstrated positive results against chronic diseases due to the presence of phytochemicals, mainly phenolic compounds. The extraction process of bioactive compounds increases the efficient collection of extracts with high bioactivity. Microwave-Assisted Extraction (MAE) constituted a "green technology" widely employed for plant matrix. In this work, the impact of temperature (50-150 °C), composition of extraction solvent (15-75% EtOH) and extraction time (5-20 min) on the extraction yield and individual compounds concentrations were evaluated. Furthermore, the characterization of 16 extracts obtained was performed by HPLC-ESI-TOF-MS. The results showed that 164 °C, 12.5 min, 45% ethanol was the best extraction condition, although glycoside flavonoids were degraded. Besides that, the optimal conditions for extraction yield were 164 °C, 60% ethanol and 22 min. Thus, temperature and solvent concentration have demonstrated to be potential factors in MAE for obtaining bioactive compounds from H. sabdariffa. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/29734100/Microwave_assisted_extraction_for_Hibiscus_sabdariffa_bioactive_compounds_ DB - PRIME DP - Unbound Medicine ER -