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Adding a purple corn extract in rats supplemented with chia oil decreases gene expression of SREBP-1c and retains Δ5 and Δ6 hepatic desaturase activity, unmodified the hepatic lipid profile.

Abstract

Flavonoids upregulate gene expression of PPAR-α and underregulate the gene expression of SREBP-1c, and their intake increases the plasmatic concentration of n-3 LC-PUFAs. However, the biological mechanisms underlying these effects have not been elucidated. In this work, the effect of oral supplementation of ALA from chia (Salvia hispanica L.) seed oil and anthocyanins from a purple corn extract (PCE) on gene expression of SREBP-1c, PPAR-α and Δ5 and Δ6 desaturases (Δ5D and Δ6D), the activity of these enzymes in the liver as well as the hepatic lipid profile were evaluated in thirty-six female Sprague Dawley rats whose diet was supplemented with olive oil (OL), chia oil (CH), olive oil and PCE (OL + PCE) or chia oil and PCE (CH + PCE). Gene expression of PPAR-α was significantly higher when supplemented with CH and CH + PCE, SREBP-1c gene expression was higher when supplemented with chia oil. CH supplementation enhanced Δ5D expression whereas no significant differences between treatments were observed concerning Δ6D gene expression. Activities of both desaturases were increased by including olive oil (OL + PCE and OL), and they were found to be higher in CH + PCE respect to CH for both enzymes. The ALA and n-3 LCPUFAs hepatic content was higher with CH, decreasing the levels of AA and n-6 LCPUFAs. It is concluded that the joint action of flavonoids such as anthocyanins and ALA show an anti-adipogenic effect. Desaturase activity was inhibited by ALA and kept by the anthocyanins from PCE, thus anthocyanins would exert a protective effect on the desaturase activity but they would not affect on its gene expression, however, high doses of ALA increased the production of its metabolites, masking the effect of PCE.

Authors+Show Affiliations

Nutrition Department, Animal Husbandry Faculty, Universidad Nacional Agraria La Molina, Lima, Perú; Carrera de Medicina Veterinaria, Facultad de Ciencias Veterinarias, Universidad Técnica de Manabí, Manabí, Ecuador. Electronic address: sreyna@utm.edu.ec.Nutrition Department, Animal Husbandry Faculty, Universidad Nacional Agraria La Molina, Lima, Perú.Nutrition Department, Faculty of Medicine, Universidad de Chile, Santiago, Chile; Lipid Center, Institute of Nutrition and Food Technology (INTA), University of Chile, Santiago, Chile.Nutrition Department, Faculty of Medicine, Universidad de Chile, Santiago, Chile.Nutrition Department, Animal Husbandry Faculty, Universidad Nacional Agraria La Molina, Lima, Perú.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29735017

Citation

Reyna Gallegos, Sixto, et al. "Adding a Purple Corn Extract in Rats Supplemented With Chia Oil Decreases Gene Expression of SREBP-1c and Retains Δ5 and Δ6 Hepatic Desaturase Activity, Unmodified the Hepatic Lipid Profile." Prostaglandins, Leukotrienes, and Essential Fatty Acids, vol. 132, 2018, pp. 1-7.
Reyna Gallegos S, Torres Arrunátegui G, Valenzuela R, et al. Adding a purple corn extract in rats supplemented with chia oil decreases gene expression of SREBP-1c and retains Δ5 and Δ6 hepatic desaturase activity, unmodified the hepatic lipid profile. Prostaglandins Leukot Essent Fatty Acids. 2018;132:1-7.
Reyna Gallegos, S., Torres Arrunátegui, G., Valenzuela, R., Rincón-Cervera, M. Á., & Villanueva Espinoza, M. E. (2018). Adding a purple corn extract in rats supplemented with chia oil decreases gene expression of SREBP-1c and retains Δ5 and Δ6 hepatic desaturase activity, unmodified the hepatic lipid profile. Prostaglandins, Leukotrienes, and Essential Fatty Acids, 132, 1-7. https://doi.org/10.1016/j.plefa.2018.03.005
Reyna Gallegos S, et al. Adding a Purple Corn Extract in Rats Supplemented With Chia Oil Decreases Gene Expression of SREBP-1c and Retains Δ5 and Δ6 Hepatic Desaturase Activity, Unmodified the Hepatic Lipid Profile. Prostaglandins Leukot Essent Fatty Acids. 2018;132:1-7. PubMed PMID: 29735017.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Adding a purple corn extract in rats supplemented with chia oil decreases gene expression of SREBP-1c and retains Δ5 and Δ6 hepatic desaturase activity, unmodified the hepatic lipid profile. AU - Reyna Gallegos,Sixto, AU - Torres Arrunátegui,Génesis, AU - Valenzuela,Rodrigo, AU - Rincón-Cervera,Miguel Ángel, AU - Villanueva Espinoza,María Elena, Y1 - 2018/03/28/ PY - 2018/02/04/received PY - 2018/03/21/accepted PY - 2018/5/9/entrez PY - 2018/5/8/pubmed PY - 2019/2/7/medline KW - Chia oil KW - PPAR KW - Purple corn KW - SREBP KW - Δ5/Δ6 desaturase activity SP - 1 EP - 7 JF - Prostaglandins, leukotrienes, and essential fatty acids JO - Prostaglandins Leukot. Essent. Fatty Acids VL - 132 N2 - Flavonoids upregulate gene expression of PPAR-α and underregulate the gene expression of SREBP-1c, and their intake increases the plasmatic concentration of n-3 LC-PUFAs. However, the biological mechanisms underlying these effects have not been elucidated. In this work, the effect of oral supplementation of ALA from chia (Salvia hispanica L.) seed oil and anthocyanins from a purple corn extract (PCE) on gene expression of SREBP-1c, PPAR-α and Δ5 and Δ6 desaturases (Δ5D and Δ6D), the activity of these enzymes in the liver as well as the hepatic lipid profile were evaluated in thirty-six female Sprague Dawley rats whose diet was supplemented with olive oil (OL), chia oil (CH), olive oil and PCE (OL + PCE) or chia oil and PCE (CH + PCE). Gene expression of PPAR-α was significantly higher when supplemented with CH and CH + PCE, SREBP-1c gene expression was higher when supplemented with chia oil. CH supplementation enhanced Δ5D expression whereas no significant differences between treatments were observed concerning Δ6D gene expression. Activities of both desaturases were increased by including olive oil (OL + PCE and OL), and they were found to be higher in CH + PCE respect to CH for both enzymes. The ALA and n-3 LCPUFAs hepatic content was higher with CH, decreasing the levels of AA and n-6 LCPUFAs. It is concluded that the joint action of flavonoids such as anthocyanins and ALA show an anti-adipogenic effect. Desaturase activity was inhibited by ALA and kept by the anthocyanins from PCE, thus anthocyanins would exert a protective effect on the desaturase activity but they would not affect on its gene expression, however, high doses of ALA increased the production of its metabolites, masking the effect of PCE. SN - 1532-2823 UR - https://www.unboundmedicine.com/medline/citation/29735017/Adding_a_purple_corn_extract_in_rats_supplemented_with_chia_oil_decreases_gene_expression_of_SREBP_1c_and_retains_Δ5_and_Δ6_hepatic_desaturase_activity_unmodified_the_hepatic_lipid_profile_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0952-3278(18)30033-4 DB - PRIME DP - Unbound Medicine ER -